pCLIP-gRNA-hCMV-Puro

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polIII terminator BsmBI (2505) BsmBI (4390) pCLIP-gRNA-hCMV-Puro 9019 bp
BsmBI  (2505)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BsmBI  (4390)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Stuffer
2506 .. 4390  =  1885 bp
Stuffer
2506 .. 4390  =  1885 bp
AmpR
8024 .. 8884  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8024 .. 8815  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8024 .. 8884  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8816 .. 8884  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8024 .. 8884  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
5112 .. 5711  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
5112 .. 5711  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
5725 .. 6313  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
5725 .. 6313  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
7268 .. 7853  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7268 .. 7853  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
4479 .. 4858  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4479 .. 4858  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
27 .. 404  =  378 bp
human cytomegalovirus immediate early enhancer
SV40
6850 .. 7179  =  330 bp
SV40 enhancer and early promoter
SV40
6850 .. 7179  =  330 bp
SV40 enhancer and early promoter
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
411 .. 668  =  258 bp
truncated 5' long terminal repeat (LTR) from HIV-1
RRE
1333 .. 1566  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1333 .. 1566  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
3' LTR (ΔU3)
6521 .. 6754  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
6521 .. 6754  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
CMV promoter
4859 .. 5062  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4859 .. 5062  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
715 .. 840  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2093 .. 2210  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2093 .. 2210  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
8885 .. 8989  =  105 bp
AmpR promoter
8885 .. 8989  =  105 bp
gRNA scaffold
4391 .. 4466  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
4391 .. 4466  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
4467 .. 4472  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
4467 .. 4472  =  6 bp
RNA polymerase III transcription terminator
U6 promoter
2261 .. 2509  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
2261 .. 2509  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
ORF:  5815 .. 6342  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  1211 .. 2098  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  5828 .. 6124  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  5112 .. 5711  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  8154 .. 8420  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  8781 .. 31  =  270 bp
ORF:  89 amino acids  =  10.4 kDa
ORF:  2684 .. 3079  =  396 bp
ORF:  131 amino acids  =  14.0 kDa
ORF:  8024 .. 8884  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1126 .. 1506  =  381 bp
ORF:  126 amino acids  =  13.3 kDa
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Download pCLIP-gRNA-hCMV-Puro.dna file

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