pSpCas9n(BB)-2A-GFP (PX461)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
BbsI (245) AAV2 ITR BbsI (267) polIII terminator chicken β-actin promoter ATG pSpCas9n(BB)-2A-GFP (PX461) 9288 bp
BbsI  (245)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (267)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
ATG
1250 .. 1252  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1250 .. 1252  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
3xFLAG
1253 .. 1318  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
1253 .. 1318  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
SV40 NLS
1325 .. 1345  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
1325 .. 1345  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9(D10A)
1370 .. 5470  =  4101 bp
1367 amino acids  =  158.3 kDa
3 segments
   Segment 1:  
   1370 .. 1393  =  24 bp
   8 amino acids  =  923.1 Da
Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain
Cas9(D10A)
1370 .. 5470  =  4101 bp
1367 amino acids  =  158.3 kDa
3 segments
   Segment 2:  
   1394 .. 1396  =  3 bp
   1 amino acid  =  89.1 Da
Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain
Cas9(D10A)
1370 .. 5470  =  4101 bp
1367 amino acids  =  158.3 kDa
3 segments
   Segment 3:  
   1397 .. 5470  =  4074 bp
   1358 amino acids  =  157.3 kDa
Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain
Cas9(D10A)
1370 .. 5470  =  4101 bp
1367 amino acids  =  158.3 kDa
3 segments
Product: nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain
nucleoplasmin NLS
5471 .. 5518  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
5471 .. 5518  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
T2A
5534 .. 5587  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
T2A
5534 .. 5587  =  54 bp
18 amino acids  =  1.8 kDa
Product: 2A peptide from Thosea asigna virus capsid protein
Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.
EGFP
5588 .. 6301  =  714 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 1:  1a  
   5588 .. 5590  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
5588 .. 6301  =  714 bp
238 amino acids  =  26.8 kDa
2 segments
   Segment 2:  
   5591 .. 6301  =  711 bp
   237 amino acids  =  26.7 kDa
Product: enhanced GFP
mammalian codon-optimized
EGFP
5588 .. 6301  =  714 bp
238 amino acids  =  26.8 kDa
2 segments
Product: enhanced GFP
mammalian codon-optimized
AmpR
7608 .. 8468  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7608 .. 7676  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7608 .. 8468  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7677 .. 8468  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7608 .. 8468  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
8639 .. 9227  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
8639 .. 9227  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
6766 .. 7221  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6766 .. 7221  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
440 .. 725  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
CMV enhancer
440 .. 725  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
chicken β-actin promoter
727 .. 1004  =  278 bp
chicken β-actin promoter
727 .. 1004  =  278 bp
U6 promoter
1 .. 249  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
1 .. 249  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
hybrid intron
1005 .. 1232  =  228 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
hybrid intron
1005 .. 1232  =  228 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
bGH poly(A) signal
6335 .. 6542  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
6335 .. 6542  =  208 bp
bovine growth hormone polyadenylation signal
AAV2 ITR
6551 .. 6691  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
6551 .. 6691  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
7503 .. 7607  =  105 bp
AmpR promoter
7503 .. 7607  =  105 bp
gRNA scaffold
268 .. 343  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
268 .. 343  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
344 .. 349  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
344 .. 349  =  6 bp
RNA polymerase III transcription terminator
ORF:  6565 .. 6789  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  635 .. 982  =  348 bp
ORF:  115 amino acids  =  12.1 kDa
ORF:  1250 .. 6310  =  5061 bp
ORF:  1686 amino acids  =  194.0 kDa
ORF:  825 .. 1076  =  252 bp
ORF:  83 amino acids  =  8.7 kDa
ORF:  1188 .. 1427  =  240 bp
ORF:  79 amino acids  =  9.0 kDa
ORF:  7608 .. 8468  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2716 .. 3009  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  3280 .. 3729  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  6465 .. 6746  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  1229 .. 3886  =  2658 bp
ORF:  885 amino acids  =  93.0 kDa
ORF:  8072 .. 8338  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pSpCas9n(BB)-2A-GFP (PX461).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.