PX853

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U6 promoter BbsI (245) BbsI (267) polIII terminator PX853 6295 bp
BbsI  (245)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BbsI  (267)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
ATG
1251 .. 1253  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1251 .. 1253  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Cas9(N)
1278 .. 2993  =  1716 bp
572 amino acids  =  66.3 kDa
Product: N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)
Cas9(N)
1278 .. 2993  =  1716 bp
572 amino acids  =  66.3 kDa
Product: N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)
FRB
3039 .. 3308  =  270 bp
90 amino acids  =  10.9 kDa
Product: FKBP-rapamycin binding domain of human FRAP
FRB
3039 .. 3308  =  270 bp
90 amino acids  =  10.9 kDa
Product: FKBP-rapamycin binding domain of human FRAP
AmpR
4615 .. 5475  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4615 .. 4683  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4615 .. 5475  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4684 .. 5475  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4615 .. 5475  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
5646 .. 6234  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5646 .. 6234  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3773 .. 4228  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3773 .. 4228  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
440 .. 725  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
CMV enhancer
440 .. 725  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
chicken β-actin promoter
727 .. 1004  =  278 bp
chicken β-actin promoter
727 .. 1004  =  278 bp
U6 promoter
1 .. 249  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
1 .. 249  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
hybrid intron
1005 .. 1233  =  229 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
hybrid intron
1005 .. 1233  =  229 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
bGH poly(A) signal
3342 .. 3549  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
3342 .. 3549  =  208 bp
bovine growth hormone polyadenylation signal
AAV2 ITR
3558 .. 3698  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
3558 .. 3698  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
4510 .. 4614  =  105 bp
AmpR promoter
4510 .. 4614  =  105 bp
gRNA scaffold
268 .. 343  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
268 .. 343  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
polIII terminator
344 .. 349  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
344 .. 349  =  6 bp
RNA polymerase III transcription terminator
ORF:  4615 .. 5475  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  635 .. 982  =  348 bp
ORF:  115 amino acids  =  12.1 kDa
ORF:  3572 .. 3796  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  825 .. 1136  =  312 bp
ORF:  103 amino acids  =  10.7 kDa
ORF:  1251 .. 3311  =  2061 bp
ORF:  686 amino acids  =  79.1 kDa
ORF:  2624 .. 2917  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  3472 .. 3753  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  5079 .. 5345  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download PX853.dna file

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