pX330A-1x2

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: CRISPR Plasmids | More Plasmid Sets
No matches
lac operator BsaI (8500) polIII terminator BbsI - BpiI (8341) BbsI - BpiI (8319) nucleoplasmin NLS BsaI (8959) chicken β-actin promoter ATG pX330A-1x2 8964 bp
BsaI  (8500)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BbsI  (8341)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (8341)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BbsI  (8319)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (8319)
2 sites
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BsaI  (8959)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ATG
819 .. 821  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
819 .. 821  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
3xFLAG
822 .. 887  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
822 .. 887  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
SV40 NLS
894 .. 914  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
894 .. 914  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
939 .. 5039  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
939 .. 5039  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
5040 .. 5087  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
5040 .. 5087  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
AmpR
6394 .. 7254  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6394 .. 6462  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6394 .. 7254  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6463 .. 7254  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6394 .. 7254  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7425 .. 8013  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7425 .. 8013  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5552 .. 6007  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5552 .. 6007  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
lacZα
8503 .. 8808  =  306 bp
101 amino acids  =  11.2 kDa
Product: LacZα fragment of β-galactosidase
lacZα
8503 .. 8808  =  306 bp
101 amino acids  =  11.2 kDa
Product: LacZα fragment of β-galactosidase
CMV enhancer
8 .. 293  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
CMV enhancer
8 .. 293  =  286 bp
human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer
chicken β-actin promoter
295 .. 572  =  278 bp
chicken β-actin promoter
295 .. 572  =  278 bp
U6 promoter
8075 .. 8323  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
8075 .. 8323  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
hybrid intron
573 .. 801  =  229 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
hybrid intron
573 .. 801  =  229 bp
hybrid between chicken β-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)
bGH poly(A) signal
5121 .. 5328  =  208 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
5121 .. 5328  =  208 bp
bovine growth hormone polyadenylation signal
AAV2 ITR
5337 .. 5477  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
5337 .. 5477  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
6289 .. 6393  =  105 bp
AmpR promoter
6289 .. 6393  =  105 bp
gRNA scaffold
8342 .. 8417  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
8342 .. 8417  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
lac promoter
8852 .. 8882  =  31 bp
3 segments
   Segment 3:  -10  
   8852 .. 8858  =  7 bp
promoter for the E. coli lac operon
lac promoter
8852 .. 8882  =  31 bp
3 segments
   Segment 2:  
   8859 .. 8876  =  18 bp
promoter for the E. coli lac operon
lac promoter
8852 .. 8882  =  31 bp
3 segments
   Segment 1:  -35  
   8877 .. 8882  =  6 bp
promoter for the E. coli lac operon
lac promoter
8852 .. 8882  =  31 bp
3 segments
promoter for the E. coli lac operon
lac operator
8828 .. 8844  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8828 .. 8844  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
polIII terminator
8418 .. 8423  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
8418 .. 8423  =  6 bp
RNA polymerase III transcription terminator
ORF:  757 .. 996  =  240 bp
ORF:  79 amino acids  =  9.1 kDa
ORF:  6394 .. 7254  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  203 .. 550  =  348 bp
ORF:  115 amino acids  =  12.1 kDa
ORF:  5351 .. 5575  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  8897 .. 187  =  255 bp
ORF:  84 amino acids  =  9.2 kDa
ORF:  393 .. 704  =  312 bp
ORF:  103 amino acids  =  10.7 kDa
ORF:  819 .. 5090  =  4272 bp
ORF:  1423 amino acids  =  164.6 kDa
ORF:  5251 .. 5532  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  8503 .. 8808  =  306 bp
ORF:  101 amino acids  =  11.2 kDa
ORF:  798 .. 3455  =  2658 bp
ORF:  885 amino acids  =  93.1 kDa
ORF:  6858 .. 7124  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2285 .. 2578  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  2849 .. 3298  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
Click here to try SnapGene

Download pX330A-1x2.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.