pGuide-it-tdTomato Vector (Linear)

Linearized CRISPR/Cas9 vector with a tdTomato marker, for cloning and expression of a single guide RNA (sgRNA).

Sequence Author: Clontech (TaKaRa)

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Guide-it™ Sequencing Primer 1 (8959 .. 8982) FLAG SV40 NLS polIII terminator pGuide-it-tdTomato Vector (Linear) 9038 bp
Guide-it™ Sequencing Primer 1
24-mer  /  38% GC
1 binding site
8959 .. 8982  =  24 annealed bases
Tm  =  56°C
Cas9
4286 .. 8389  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
4286 .. 8389  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
8405 .. 8425  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
8405 .. 8425  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
FLAG
8426 .. 8449  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
8426 .. 8449  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
tdTomato
2126 .. 3556  =  1431 bp
476 amino acids  =  54.2 kDa
Product: tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)
mammalian codon-optimized
tdTomato
2126 .. 3556  =  1431 bp
476 amino acids  =  54.2 kDa
Product: tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)
mammalian codon-optimized
AmpR
908 .. 1768  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   908 .. 1699  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
908 .. 1768  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   1700 .. 1768  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
908 .. 1768  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
149 .. 737  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
149 .. 737  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
3709 .. 4012  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3709 .. 4012  =  304 bp
human cytomegalovirus immediate early enhancer
U6 promoter
8792 .. 9040  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
U6 promoter
8792 .. 9040  =  249 bp
RNA polymerase III promoter for human U6 snRNA (Domitrovich & Kunkel, 2003)
CMV promoter
4013 .. 4216  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4013 .. 4216  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
1769 .. 1873  =  105 bp
AmpR promoter
1769 .. 1873  =  105 bp
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
gRNA scaffold
1 .. 86  =  86 bp
guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system
SV40 poly(A) signal
1906 .. 1987  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1906 .. 1987  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8590 .. 8671  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8590 .. 8671  =  82 bp
SV40 polyadenylation signal
minimal CMV promoter
3597 .. 3634  =  38 bp
human cytomegalovirus (CMV) immediate early promoter
minimal CMV promoter
3597 .. 3634  =  38 bp
human cytomegalovirus (CMV) immediate early promoter
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
87 .. 92  =  6 bp
RNA polymerase III transcription terminator
ORF:  2078 .. 3658  =  1581 bp
ORF:  526 amino acids  =  52.7 kDa
ORF:  4286 .. 8452  =  4167 bp
ORF:  1388 amino acids  =  160.7 kDa
ORF:  1038 .. 1304  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5635 .. 5928  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  6250 .. 6648  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  2109 .. 3641  =  1533 bp
ORF:  510 amino acids  =  56.9 kDa
ORF:  908 .. 1768  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  2126 .. 3556  =  1431 bp
ORF:  476 amino acids  =  54.2 kDa
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Download pGuide-it-tdTomato Vector (Linear).dna file

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