pExpress-1

Mammalian expression vector for constructing cDNA libraries.

Sequence Author: I.M.A.G.E. Consortium

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HomePlasmidsI.M.A.G.E. Consortium PlasmidspExpress-1
BspDI * - ClaI * (123) NaeI (3988) NgoMIV (3986) DraIII (3885) XmnI (3341) ScaI (3222) NmeAIII (2890) BsaI (2803) AhdI (2742) AlwNI (2265) PspFI (2157) BseYI (2153) SV40 poly(A) signal HincII - HpaI (259) MfeI (268) BsaBI * (360) BclI * (361) NheI (637) BmtI (641) M13 fwd T7 promoter BseRI (706) AbsI - PaeR7I - PspXI - XhoI (718) PacI (729) EagI - NotI (734) EcoRV (743) TspMI - XmaI (746) SmaI (748) EcoRI (751) RsrII (758) SP6 promoter StuI (793) M13 rev SacII (840) AvrII (843) BbsI (872) BsmBI - Esp3I (917) Eco53kI (944) SacI (946) CMV promoter NcoI (1146) SnaBI (1170) NsiI (1457) BstAPI (1673) BspQI - SapI (1733) AflIII - PciI (1849) pExpress-1 4117 bp
BspDI  (123)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (123)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
NaeI  (3988)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3986)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
DraIII  (3885)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
XmnI  (3341)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (3222)
1 site		 A G T A C T T C A T G A
NmeAIII  (2890)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaI  (2803)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2742)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2265)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PspFI  (2157)
1 site		 C C C A G C G G G T C G
BseYI  (2153)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
HincII  (259)
1 site		 G T Y R A C C A R Y T G
HpaI  (259)
1 site		 G T T A A C C A A T T G
MfeI  (268)
1 site		 C A A T T G G T T A A C
BsaBI  (360)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BclI  (361)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
NheI  (637)
1 site		 G C T A G C C G A T C G
BmtI  (641)
1 site		 G C T A G C C G A T C G
BseRI  (706)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AbsI  (718)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (718)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (718)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (718)
1 site		 C T C G A G G A G C T C
PacI  (729)
1 site		 T T A A T T A A A A T T A A T T
EagI  (734)
1 site		 C G G C C G G C C G G C
NotI  (734)
1 site		 G C G G C C G C C G C C G G C G
EcoRV  (743)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
TspMI  (746)
1 site		 C C C G G G G G G C C C
XmaI  (746)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (748)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
EcoRI  (751)
1 site		 G A A T T C C T T A A G
RsrII  (758)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
StuI  (793)
1 site		 A G G C C T T C C G G A
SacII  (840)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
AvrII  (843)
1 site		 C C T A G G G G A T C C
BbsI  (872)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsmBI  (917)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (917)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
Eco53kI  (944)
1 site		 G A G C T C C T C G A G
SacI  (946)
1 site		 G A G C T C C T C G A G
NcoI  (1146)
1 site		 C C A T G G G G T A C C
SnaBI  (1170)
1 site		 T A C G T A A T G C A T
NsiI  (1457)
1 site		 A T G C A T T A C G T A
BstAPI  (1673)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BspQI  (1733)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1733)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (1849)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (1849)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AmpR
2669 .. 3529  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2669 .. 3460  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2669 .. 3529  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3461 .. 3529  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2669 .. 3529  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1910 .. 2498  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1910 .. 2498  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3661 .. 4116  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3661 .. 4116  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
1147 .. 1450  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1147 .. 1450  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
943 .. 1146  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
943 .. 1146  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
125 .. 259  =  135 bp
SV40 polyadenylation signal
AmpR promoter
3530 .. 3634  =  105 bp
AmpR promoter
3530 .. 3634  =  105 bp
MCS
718 .. 763  =  46 bp
multiple cloning site
MCS
718 .. 763  =  46 bp
multiple cloning site
T7 promoter
691 .. 709  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
691 .. 709  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
772 .. 790  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
772 .. 790  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
664 .. 680  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
801 .. 817  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
801 .. 817  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  798 .. 1064  =  267 bp
ORF:  88 amino acids  =  9.5 kDa
ORF:  2799 .. 3065  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1253 .. 1510  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  2669 .. 3529  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

Lafmid BA
pcDNAII
pCR2.1-TOPO (linearized)
pExpress-1
pAD-GAL4
pcDNA3.1(+)
pCR3.1
pME18S-FL3
pAMP1
pcDNA3.1(-)
pCR3.1 (linearized)
pOTB7
pAMP10
pcDNA3.1 Zeo(+)
pCR4-TOPO
pOTB7-3
pBK-CMV
pCMV SPORT
pCR4-TOPO (linearized)
pOTB7a
pBluescribe (modified)
pCMV SPORT2
pCS105
pPCR-Script Amp SK(+)
pBluescript II SK(+)
pCMV SPORT4
pCS107
pRKW2
pBluescript KS(+)
pCMV SPORT6
pCS108
pSPORT 1
pBluescript SK(+)
pCMV SPORT6.1
pCS2+
pSPORT 2
pBluescript SK(-)
pCMV SPORT6ccdB
pCS22+
pT7T3D-PacI
pBluescript-FL
pCR-Blunt II-TOPO
pCS2G
pUC19
pBluescript II KS(+)
pCR-Blunt II-TOPO (linearized)
pDNR-Dual
pYX-Asc
pBluescriptR
pCR-XL-TOPO
pDNR-LIB
pZErO-2
pcDNA3
pCR-XL-TOPO (linearized)
pDONR201
pZL1
pcDNAI
pCR2.1-TOPO
pDONR223

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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