pSPORT 1

Vector for cloning, sequencing, and bacterial expression of cDNAs.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free

|Download SnapGene Viewer

Explore Over 2.7k Plasmids: I.M.A.G.E. Consortium Plasmids | More Plasmid Sets

No matches

HomePlasmidsI.M.A.G.E. Consortium PlasmidspSPORT 1

Show Static Map

NaeI (3980)
1 site

Efficient cleavage requires at least two copies of the NaeI recognition sequence.

NgoMIV (3978)
1 site

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.

DraIII (3877)
1 site

Sticky ends from different DraIII sites may not be compatible.

PsiI (3749)
1 site

XmnI (3333)
1 site

ScaI (3214)
1 site

TatI (3212)
1 site

BsaI (2795)
1 site

Sticky ends from different BsaI sites may not be compatible.

BsaI can be used between 37°C and 50°C.

AhdI (2734)
1 site

The 1-base overhangs produced by AhdI may be hard to ligate.

Sticky ends from different AhdI sites may not be compatible.

AlwNI (2257)
1 site

Sticky ends from different AlwNI sites may not be compatible.

ZraI (193)
1 site

AatII (195)
1 site

SphI (201)
1 site

MluI (203)
1 site

BsiWI (206)
1 site

BsiWI is typically used at 55°C, but is 50% active at 37°C.

SnaBI (210)
1 site

HindIII (213)
1 site

BamHI (219)
1 site

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

XbaI (225)
1 site

EagI (232)
1 site

NotI (232)
1 site

SpeI (241)
1 site

Eco53kI (249)
1 site

SacI (251)
1 site

SalI (253)
1 site

AccI (254)
1 site

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.

Sticky ends from different AccI sites may not be compatible.

AvaI (258)
1 site

Sticky ends from different AvaI sites may not be compatible.

BsoBI (258)
1 site

Sticky ends from different BsoBI sites may not be compatible.

BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.

TspMI (258)
1 site

XmaI (258)
1 site

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.

SmaI (260)
1 site

SmaI can be used at 37°C for brief incubations.

EcoRI (263)
1 site

BspEI (267)
1 site

RsrII (270)
1 site

Efficient cleavage requires at least two copies of the RsrII recognition sequence.

Sticky ends from different RsrII sites may not be compatible.

For full activity, add fresh DTT.

AgeI (272)
1 site

Acc65I (275)
1 site

KpnI (279)
1 site

PstI (284)
1 site

SbfI (284)
1 site

BfuAI (287)
1 site

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.

Sticky ends from different BfuAI sites may not be compatible.

BfuAI is typically used at 50°C, but is 50% active at 37°C.

BspMI (287)
1 site

Efficient cleavage requires at least two copies of the BspMI recognition sequence.

Sticky ends from different BspMI sites may not be compatible.

KasI (552)
1 site

NarI (553)
1 site

* Blocked by Dcm methylation.

Efficient cleavage requires at least two copies of the NarI recognition sequence.

SfoI (554)
1 site

PluTI (556)
1 site

Efficient cleavage requires at least two copies of the PluTI recognition sequence.

BsmBI (576)
1 site

Sticky ends from different BsmBI sites may not be compatible.

BsmBI-v2 is an improved version of BsmBI.

Esp3I (576)
1 site

Sticky ends from different Esp3I sites may not be compatible.

EcoRV (745)
1 site

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.

PspOMI (984)
1 site

ApaI (988)
1 site

ApaI can be used between 25°C and 37°C.

BstEII (1009)
1 site

Sticky ends from different BstEII sites may not be compatible.

BstEII is typically used at 60°C, but is 50% active at 37°C.

BclI (1177)
1 site

* Blocked by Dam methylation.

BclI is typically used at 50-55°C, but is 50% active at 37°C.

PflMI (1616)
1 site

Sticky ends from different PflMI sites may not be compatible.

MauBI (1648)
1 site

PciI (1841)
1 site

PciI is inhibited by nonionic detergents.

lacI
464 .. 1546 = 1083 bp
360 amino acids = 38.5 kDa

Product: lac repressor

The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lacI
464 .. 1546 = 1083 bp
360 amino acids = 38.5 kDa

Product: lac repressor

The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

AmpR
2661 .. 3521 = 861 bp
286 amino acids = 31.6 kDa
2 segments

   Segment 2:
   2661 .. 3452  =  792 bp
   263 amino acids  =  28.9 kDa

Product: β-lactamase

confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR
2661 .. 3521 = 861 bp
286 amino acids = 31.6 kDa
2 segments

   Segment 1:  signal sequence
   3453 .. 3521  =  69 bp
   23 amino acids  =  2.6 kDa

Product: β-lactamase

confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR
2661 .. 3521 = 861 bp
286 amino acids = 31.6 kDa
2 segments

Product: β-lactamase

confers resistance to ampicillin, carbenicillin, and related antibiotics

ori
1902 .. 2490 = 589 bp

high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori
1902 .. 2490 = 589 bp

high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

f1 ori
3653 .. 4108 = 456 bp

f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

f1 ori
3653 .. 4108 = 456 bp

f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

AmpR promoter
3522 .. 3626 = 105 bp

MCS
191 .. 286 = 96 bp

multiple cloning site

MCS
191 .. 286 = 96 bp

multiple cloning site

lacI promoter
1547 .. 1624 = 78 bp

lac promoter
385 .. 415 = 31 bp
3 segments

Segment 3: -10
385 .. 391 = 7 bp

promoter for the E. coli lac operon

lac promoter
385 .. 415 = 31 bp
3 segments

Segment 2:
392 .. 409 = 18 bp

promoter for the E. coli lac operon

lac promoter
385 .. 415 = 31 bp
3 segments

Segment 1: -35
410 .. 415 = 6 bp

promoter for the E. coli lac operon

lac promoter
385 .. 415 = 31 bp
3 segments

promoter for the E. coli lac operon

SP6 promoter
164 .. 182 = 19 bp

promoter for bacteriophage SP6 RNA polymerase

SP6 promoter
164 .. 182 = 19 bp

promoter for bacteriophage SP6 RNA polymerase

T7 promoter
300 .. 318 = 19 bp

promoter for bacteriophage T7 RNA polymerase

T7 promoter
300 .. 318 = 19 bp

promoter for bacteriophage T7 RNA polymerase

M13 fwd
140 .. 156 = 17 bp

common sequencing primer, one of multiple similar variants

M13 fwd
140 .. 156 = 17 bp

common sequencing primer, one of multiple similar variants

M13 rev
337 .. 353 = 17 bp

common sequencing primer, one of multiple similar variants

M13 rev
337 .. 353 = 17 bp

common sequencing primer, one of multiple similar variants

lac operator
361 .. 377 = 17 bp

The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator
361 .. 377 = 17 bp

The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lacZα
4085 .. 341 = 366 bp
121 amino acids = 13.7 kDa

Product: LacZα fragment of β-galactosidase

lacZα
4085 .. 341 = 366 bp
121 amino acids = 13.7 kDa

Product: LacZα fragment of β-galactosidase

ORF: 430 .. 681 = 252 bp
ORF: 83 amino acids = 9.1 kDa

ORF: 2791 .. 3057 = 267 bp
ORF: 88 amino acids = 9.2 kDa

ORF: 4085 .. 341 = 366 bp
ORF: 121 amino acids = 13.7 kDa

ORF: 444 .. 707 = 264 bp
ORF: 87 amino acids = 8.9 kDa

ORF: 1296 .. 1613 = 318 bp
ORF: 105 amino acids = 11.2 kDa

ORF: 2661 .. 3521 = 861 bp
ORF: 286 amino acids = 31.6 kDa

ORF: 464 .. 1423 = 960 bp
ORF: 319 amino acids = 34.1 kDa

Click here to try SnapGene

Download pSPORT 1.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

Fast accurate construct design for all major molecular cloning techniques
Validate sequenced constructs using powerful alignment tools
Customize plasmid maps with flexible annotation and visualization controls
Automatically generate a rich graphical history of every edit and procedure

Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

Gain unparalleled visibility of your plasmids, DNA and protein sequences
Annotate features on your plasmids using the curated feature database
Store, search, and share your sequences, files and maps

Download SnapGene Viewer

Individual Sequences & Maps

Lafmid BA

pcDNAII

pCR2.1-TOPO (linearized)

pCR4-TOPO (linearized)

pOTB7a

pBluescribe (modified)

pCMV SPORT2

pCS105

pPCR-Script Amp SK(+)

pCR-Blunt II-TOPO (linearized)

pCR-XL-TOPO (linearized)

Plasmid Sets

Basic Cloning Vectors

I.M.A.G.E. Consortium Plasmids

pET & Duet Vectors (Novagen)

TA and GC Cloning Vectors

Coronavirus Resources

Insect Cell Vectors

pGEX Vectors (GE Healthcare)

Viral Expression & Packaging Vectors

Fluorescent Protein Genes & Plasmids

Lucigen Vectors

Qiagen Vectors

Yeast Plasmids

Gateway® Cloning Vectors

Mammalian Expression Vectors

Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.

Try for Free