pYX-Asc

Compact cDNA cloning vector.

Sequence Author: I.M.A.G.E. Consortium

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HomePlasmidsI.M.A.G.E. Consortium PlasmidspYX-Asc
SspI (0) EarI (1682) XmnI (1486) DraI (1464) BsaHI (1424) ScaI (1367) TatI (1365) TsoI (1286) PvuI (1257) FspI (1109) NmeAIII (1035) BsrFI (967) BpmI (957) BsaI (948) BmrI (927) AhdI (887) PacI (6) EcoO109I - PpuMI (22) Bsu36I (41) I-CeuI (44) BsrBI (53) EagI - NotI (54) BtgI (57) SacII (60) BglI - SfiI (68) EcoRV (76) ApoI - EcoRI (80) AscI - BssHII (87) SalI (94) AccI (95) HincII (96) AflIII - PciI (156) NspI (160) DrdI (264) BciVI (359) HaeII (404) BseYI (460) PspFI (464) AlwNI (572) BanI (835) pYX-Asc 1691 bp
SspI  (0)
1 site		 A A T A T T T T A T A A
EarI  (1682)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
XmnI  (1486)
1 site		 G A A N N N N T T C C T T N N N N A A G
DraI  (1464)
1 site		 T T T A A A A A A T T T
BsaHI  (1424)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
ScaI  (1367)
1 site		 A G T A C T T C A T G A
TatI  (1365)
1 site		 W G T A C W W C A T G W
TsoI  (1286)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuI  (1257)
1 site		 C G A T C G G C T A G C
FspI  (1109)
1 site		 T G C G C A A C G C G T
NmeAIII  (1035)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (967)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BpmI  (957)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (948)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (927)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (887)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PacI  (6)
1 site		 T T A A T T A A A A T T A A T T
EcoO109I  (22)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (22)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
Bsu36I  (41)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
I-CeuI  (44)
1 site		 T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T
I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences.
BsrBI  (53)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
EagI  (54)
1 site		 C G G C C G G C C G G C
NotI  (54)
1 site		 G C G G C C G C C G C C G G C G
BtgI  (57)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
SacII  (60)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BglI  (68)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
SfiI  (68)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (76)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApoI  (80)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (80)
1 site		 G A A T T C C T T A A G
AscI  (87)
1 site		 G G C G C G C C C C G C G C G G
BssHII  (87)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
SalI  (94)
1 site		 G T C G A C C A G C T G
AccI  (95)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (96)
1 site		 G T Y R A C C A R Y T G
AflIII  (156)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (156)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
NspI  (160)
1 site		 R C A T G Y Y G T A C R
DrdI  (264)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BciVI  (359)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
HaeII  (404)
1 site		 R G C G C Y Y C G C G R
BseYI  (460)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (464)
1 site		 C C C A G C G G G T C G
AlwNI  (572)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BanI  (835)
1 site		 G G Y R C C C C R Y G G
Sticky ends from different BanI sites may not be compatible.
AmpR
814 .. 1674  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   814 .. 1605  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
814 .. 1674  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1606 .. 1674  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
814 .. 1674  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
217 .. 805  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
217 .. 805  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
MCS
40 .. 99  =  60 bp
multiple cloning site
MCS
40 .. 99  =  60 bp
multiple cloning site
T3 promoter
4 .. 22  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T3 promoter
4 .. 22  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T7 promoter
137 .. 155  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
137 .. 155  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  944 .. 1210  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  814 .. 1674  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

Lafmid BA
pcDNAII
pCR2.1-TOPO (linearized)
pExpress-1
pAD-GAL4
pcDNA3.1(+)
pCR3.1
pME18S-FL3
pAMP1
pcDNA3.1(-)
pCR3.1 (linearized)
pOTB7
pAMP10
pcDNA3.1 Zeo(+)
pCR4-TOPO
pOTB7-3
pBK-CMV
pCMV SPORT
pCR4-TOPO (linearized)
pOTB7a
pBluescribe (modified)
pCMV SPORT2
pCS105
pPCR-Script Amp SK(+)
pBluescript II SK(+)
pCMV SPORT4
pCS107
pRKW2
pBluescript KS(+)
pCMV SPORT6
pCS108
pSPORT 1
pBluescript SK(+)
pCMV SPORT6.1
pCS2+
pSPORT 2
pBluescript SK(-)
pCMV SPORT6ccdB
pCS22+
pT7T3D-PacI
pBluescript-FL
pCR-Blunt II-TOPO
pCS2G
pUC19
pBluescript II KS(+)
pCR-Blunt II-TOPO (linearized)
pDNR-Dual
pYX-Asc
pBluescriptR
pCR-XL-TOPO
pDNR-LIB
pZErO-2
pcDNA3
pCR-XL-TOPO (linearized)
pDONR201
pZL1
pcDNAI
pCR2.1-TOPO
pDONR223

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