pUC19

Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning.

Sequence Author: New England Biolabs

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HomePlasmidsI.M.A.G.E. Consortium PlasmidspUC19
EcoO109I (2675) AatII (2621) ZraI (2619) SspI (2503) XmnI (2298) ScaI (2179) TsoI (2098) NmeAIII (1847) BsrFI (1779) BpmI (1769) BsaI (1760) AhdI (1699) PfoI (46) NdeI (184) BstAPI (185) KasI (235) NarI (236) SfoI (237) PluTI (239) ApoI - EcoRI (396) Eco53kI (404) BanII - SacI (406) Acc65I (408) AvaI - BsoBI - KpnI - TspMI - XmaI (412) SmaI (414) BamHI (417) XbaI (423) SalI (429) AccI (430) HincII (431) PstI - SbfI (439) BfuAI - BspMI (442) SphI (445) HindIII (447) BspQI - SapI (690) AflIII - PciI (806) BseYI (1110) PspFI (1114) AlwNI (1222) pUC19 2686 bp
EcoO109I  (2675)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
AatII  (2621)
1 site		 G A C G T C C T G C A G
ZraI  (2619)
1 site		 G A C G T C C T G C A G
SspI  (2503)
1 site		 A A T A T T T T A T A A
XmnI  (2298)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (2179)
1 site		 A G T A C T T C A T G A
TsoI  (2098)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (1847)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (1779)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BpmI  (1769)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (1760)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (1699)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PfoI  (46)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
NdeI  (184)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstAPI  (185)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
KasI  (235)
1 site		 G G C G C C C C G C G G
NarI  (236)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (237)
1 site		 G G C G C C C C G C G G
PluTI  (239)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
ApoI  (396)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (396)
1 site		 G A A T T C C T T A A G
Eco53kI  (404)
1 site		 G A G C T C C T C G A G
BanII  (406)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (406)
1 site		 G A G C T C C T C G A G
Acc65I  (408)
1 site		 G G T A C C C C A T G G
AvaI  (412)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (412)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
KpnI  (412)
1 site		 G G T A C C C C A T G G
TspMI  (412)
1 site		 C C C G G G G G G C C C
XmaI  (412)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (414)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (417)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (423)
1 site		 T C T A G A A G A T C T
SalI  (429)
1 site		 G T C G A C C A G C T G
AccI  (430)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (431)
1 site		 G T Y R A C C A R Y T G
PstI  (439)
1 site		 C T G C A G G A C G T C
SbfI  (439)
1 site		 C C T G C A G G G G A C G T C C
BfuAI  (442)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (442)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (445)
1 site		 G C A T G C C G T A C G
HindIII  (447)
1 site		 A A G C T T T T C G A A
BspQI  (690)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (690)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (806)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (806)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BseYI  (1110)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (1114)
1 site		 C C C A G C G G G T C G
AlwNI  (1222)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AmpR
1626 .. 2486  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1626 .. 2417  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1626 .. 2486  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2418 .. 2486  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1626 .. 2486  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
867 .. 1455  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
867 .. 1455  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
lacZα
146 .. 469  =  324 bp
107 amino acids  =  12.2 kDa
Product: LacZα fragment of β-galactosidase
lacZα
146 .. 469  =  324 bp
107 amino acids  =  12.2 kDa
Product: LacZα fragment of β-galactosidase
AmpR promoter
2487 .. 2591  =  105 bp
AmpR promoter
2487 .. 2591  =  105 bp
lac promoter
513 .. 543  =  31 bp
3 segments
   Segment 3:  -10  
   513 .. 519  =  7 bp
promoter for the E. coli lac operon
lac promoter
513 .. 543  =  31 bp
3 segments
   Segment 2:  
   520 .. 537  =  18 bp
promoter for the E. coli lac operon
lac promoter
513 .. 543  =  31 bp
3 segments
   Segment 1:  -35  
   538 .. 543  =  6 bp
promoter for the E. coli lac operon
lac promoter
513 .. 543  =  31 bp
3 segments
promoter for the E. coli lac operon
lac operator
489 .. 505  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
489 .. 505  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
396 .. 452  =  57 bp
pUC19 multiple cloning site
MCS
396 .. 452  =  57 bp
pUC19 multiple cloning site
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
465 .. 481  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
465 .. 481  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  1756 .. 2022  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  186 .. 542  =  357 bp
ORF:  118 amino acids  =  13.5 kDa
ORF:  146 .. 469  =  324 bp
ORF:  107 amino acids  =  12.2 kDa
ORF:  1626 .. 2486  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

Lafmid BA
pcDNAII
pCR2.1-TOPO (linearized)
pExpress-1
pAD-GAL4
pcDNA3.1(+)
pCR3.1
pME18S-FL3
pAMP1
pcDNA3.1(-)
pCR3.1 (linearized)
pOTB7
pAMP10
pcDNA3.1 Zeo(+)
pCR4-TOPO
pOTB7-3
pBK-CMV
pCMV SPORT
pCR4-TOPO (linearized)
pOTB7a
pBluescribe (modified)
pCMV SPORT2
pCS105
pPCR-Script Amp SK(+)
pBluescript II SK(+)
pCMV SPORT4
pCS107
pRKW2
pBluescript KS(+)
pCMV SPORT6
pCS108
pSPORT 1
pBluescript SK(+)
pCMV SPORT6.1
pCS2+
pSPORT 2
pBluescript SK(-)
pCMV SPORT6ccdB
pCS22+
pT7T3D-PacI
pBluescript-FL
pCR-Blunt II-TOPO
pCS2G
pUC19
pBluescript II KS(+)
pCR-Blunt II-TOPO (linearized)
pDNR-Dual
pYX-Asc
pBluescriptR
pCR-XL-TOPO
pDNR-LIB
pZErO-2
pcDNA3
pCR-XL-TOPO (linearized)
pDONR201
pZL1
pcDNAI
pCR2.1-TOPO
pDONR223

Plasmid Sets

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TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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