pET-3d
Bacterial expression vector with a BamHI cloning site. Same reading frame as pET-3c but with an NcoI site at the start codon.
Sequence Author: MilliporeSigma (Novagen)
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ApoI is typically used at 50°C, but is 50% active at 37°C. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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Sticky ends from different AflIII sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different Esp3I sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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Sticky ends from different BstAPI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Sticky ends from different BsmI sites may not be compatible. |
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Sticky ends from different AvaI sites may not be compatible. |
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Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C. |
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* Blocked by Dcm methylation. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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* Blocked by EcoKI methylation. Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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AmpR 3569 .. 4429 = 861 bp 286 amino acids = 31.5 kDa 2 segments Segment 2: 3569 .. 4360 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3569 .. 4429 = 861 bp 286 amino acids = 31.5 kDa 2 segments Segment 1: signal sequence 4361 .. 4429 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3569 .. 4429 = 861 bp 286 amino acids = 31.5 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
ori 2810 .. 3398 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 2810 .. 3398 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
rop 2189 .. 2380 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein |
rop 2189 .. 2380 = 192 bp 63 amino acids = 7.2 kDa Product: Rop protein |
AmpR promoter 4430 .. 4534 = 105 bp |
AmpR promoter 4430 .. 4534 = 105 bp |
T7 terminator 404 .. 451 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
T7 terminator 404 .. 451 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
T7 tag 517 .. 549 = 33 bp 11 amino acids = 1.1 kDa Product: epitope tag from a T7 major capsid protein |
T7 tag 517 .. 549 = 33 bp 11 amino acids = 1.1 kDa Product: epitope tag from a T7 major capsid protein |
T7 promoter 610 .. 628 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 610 .. 628 = 19 bp promoter for bacteriophage T7 RNA polymerase |
RBS 557 .. 562 = 6 bp ribosome binding site |
RBS 557 .. 562 = 6 bp ribosome binding site |
ORF: 86 .. 451 = 366 bp ORF: 121 amino acids = 12.9 kDa |
ORF: 1454 .. 1792 = 339 bp ORF: 112 amino acids = 12.6 kDa |
ORF: 2156 .. 2380 = 225 bp ORF: 74 amino acids = 8.5 kDa |
ORF: 765 .. 1550 = 786 bp ORF: 261 amino acids = 27.5 kDa |
ORF: 3699 .. 3965 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 570 .. 839 = 270 bp ORF: 89 amino acids = 9.3 kDa |
ORF: 897 .. 1355 = 459 bp ORF: 152 amino acids = 16.7 kDa |
ORF: 713 .. 1063 = 351 bp ORF: 116 amino acids = 12.2 kDa |
ORF: 3569 .. 4429 = 861 bp ORF: 286 amino acids = 31.5 kDa |
ORF: 1789 .. 2157 = 369 bp ORF: 122 amino acids = 14.2 kDa |
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Sticky ends from different BlpI sites may not be compatible. |
T7 tag 517 .. 549 = 33 bp 11 amino acids = 1.1 kDa Product: epitope tag from a T7 major capsid protein |
T7 terminator 404 .. 451 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
T7 terminator 404 .. 451 = 48 bp transcription terminator for bacteriophage T7 RNA polymerase |
Click here to try SnapGene |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
T7 tag 517 .. 549 = 33 bp 11 amino acids = 1.1 kDa Product: epitope tag from a T7 major capsid protein |
T7 tag 517 .. 549 = 33 bp 11 amino acids = 1.1 kDa Product: epitope tag from a T7 major capsid protein |
RBS 557 .. 562 = 6 bp ribosome binding site |
RBS 557 .. 562 = 6 bp ribosome binding site |
Click here to try SnapGene |
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RBS 557 .. 562 = 6 bp ribosome binding site |
RBS 557 .. 562 = 6 bp ribosome binding site |
T7 promoter 610 .. 628 = 19 bp promoter for bacteriophage T7 RNA polymerase |
T7 promoter 610 .. 628 = 19 bp promoter for bacteriophage T7 RNA polymerase |
Click here to try SnapGene |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Click here to try SnapGene |
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Click here to try SnapGene |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
Click here to try SnapGene |
Click here to try SnapGene |
Click here to try SnapGene |
Download pET-3d.dna file
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