pLacI

lac repressor plasmid with a p15A origin of replication.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidspET & Duet Vectors (Novagen)pLacI
MscI (3551) NcoI - StyI (3513) ScaI (3401) TatI (3399) DrdI - PflFI - Tth111I (3270) Bsu36I (3161) BsaBI * (3014) EcoO109I (2957) NaeI (2928) NgoMIV (2926) EcoNI (2812) BstAPI (2668) ApaLI (2364) AflIII - MluI (2344) BstEII (2162) NmeAIII (2144) ApaI - BanII (2141) PspOMI (2137) BssHII (1933) EcoRI (1) BsaAI (312) NheI (583) BmtI (587) AccI (595) BstZ17I (596) XmnI (639) SgrAI (669) BsrBI (757) SacII (835) BssSI - BssSαI (959) NspI (1161) XbaI (1424) AcuI (1484) KasI (1705) NarI * (1706) SfoI (1707) PluTI (1709) HincII - HpaI (1842) BciVI (1892) pLacI 3813 bp
MscI  (3551)
1 site		 T G G C C A A C C G G T
NcoI  (3513)
1 site		 C C A T G G G G T A C C
StyI  (3513)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
ScaI  (3401)
1 site		 A G T A C T T C A T G A
TatI  (3399)
1 site		 W G T A C W W C A T G W
DrdI  (3270)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PflFI  (3270)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3270)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
Bsu36I  (3161)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BsaBI  (3014)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
EcoO109I  (2957)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
NaeI  (2928)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2926)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
EcoNI  (2812)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BstAPI  (2668)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
ApaLI  (2364)
1 site		 G T G C A C C A C G T G
AflIII  (2344)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
MluI  (2344)
1 site		 A C G C G T T G C G C A
BstEII  (2162)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (2144)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (2141)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BanII  (2141)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
PspOMI  (2137)
1 site		 G G G C C C C C C G G G
BssHII  (1933)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoRI  (1)
1 site		 G A A T T C C T T A A G
BsaAI  (312)
1 site		 Y A C G T R R T G C A Y
NheI  (583)
1 site		 G C T A G C C G A T C G
BmtI  (587)
1 site		 G C T A G C C G A T C G
AccI  (595)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BstZ17I  (596)
1 site		 G T A T A C C A T A T G
XmnI  (639)
1 site		 G A A N N N N T T C C T T N N N N A A G
SgrAI  (669)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BsrBI  (757)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
SacII  (835)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BssSI  (959)
1 site		 C A C G A G G T G C T C
BssSαI  (959)
1 site		 C A C G A G G T G C T C
NspI  (1161)
1 site		 R C A T G Y Y G T A C R
XbaI  (1424)
1 site		 T C T A G A A G A T C T
AcuI  (1484)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
KasI  (1705)
1 site		 G G C G C C C C G C G G
NarI  (1706)
1 site		 G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1707)
1 site		 G G C G C C C C G C G G
PluTI  (1709)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
HincII  (1842)
1 site		 G T Y R A C C A R Y T G
HpaI  (1842)
1 site		 G T T A A C C A A T T G
BciVI  (1892)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
lacI
1617 .. 2699  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
1617 .. 2699  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
CmR
3373 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
3373 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
p15A ori
848 .. 1392  =  545 bp
Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.
p15A ori
848 .. 1392  =  545 bp
Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.
cat promoter
220 .. 322  =  103 bp
promoter of the E. coli cat gene
cat promoter
220 .. 322  =  103 bp
promoter of the E. coli cat gene
lacI promoter
2700 .. 2777  =  78 bp
lacI promoter
2700 .. 2777  =  78 bp
ORF:  763 .. 1041  =  279 bp
ORF:  92 amino acids  =  10.4 kDa
ORF:  1583 .. 1834  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  2738 .. 3076  =  339 bp
ORF:  112 amino acids  =  12.1 kDa
ORF:  3373 .. 219  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  730 .. 1002  =  273 bp
ORF:  90 amino acids  =  9.7 kDa
ORF:  1597 .. 1860  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  2449 .. 3018  =  570 bp
ORF:  189 amino acids  =  19.9 kDa
ORF:  1617 .. 2576  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
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Download pLacI.dna file

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Individual Sequences & Maps

pACYCDuet-1
pET-24a(+)
pET-3a
pET-51b(+) Ek_LIC (linearized)
pCDF-1b
pET-24b(+)
pET-3b
pET-52b(+)
pCDF-2 Ek_LIC
pET-24c(+)
pET-3c
pET-52b(+) 3C_LIC
pCDF-2 Ek_LIC (linearized)
pET-24d(+)
pET-3d
pET-52b(+) 3C_LIC (linearized)
pCDFDuet-1
pET-25b(+)
pET-40b(+)
pET-53-DEST
pCOLA-2-DEST
pET-26b(+)
pET-41 Ek_LIC
pET-54-DEST
pCOLA-3-DEST
pET-27b(+)
pET-41 Ek_LIC (linearized)
pET-55-DEST
pCOLADuet-1
pET-28a(+)
pET-41a(+)
pET-56-DEST
pET-11a
pET-28b(+)
pET-41b(+)
pET-57-DEST
pET-11b
pET-28c(+)
pET-41c(+)
pET-58-DEST
pET-11c
pET-29a(+)
pET-42a(+)
pET-59-DEST
pET-11d
pET-29b(+)
pET-42b(+)
pET-60-DEST
pET-14b
pET-29c(+)
pET-42c(+)
pET-61-DEST
pET-15b
pET-30 Ek_LIC
pET-43.1 Ek_LIC
pET-62-DEST
pET-16b
pET-30 Ek_LIC (linearized)
pET-43.1 Ek_LIC (linearized)
pET-9a
pET-17b
pET-30 Xa_LIC
pET-43.1a(+)
pET-9b
pET-19b
pET-30 Xa_LIC (linearized)
pET-43.1b(+)
pET-9c
pET-20b(+)
pET-30a(+)
pET-43.1c(+)
pET-9d
pET-21(+)
pET-30b(+)
pET-44a(+)
pETBlue-1
pET-21a(+)
pET-30c(+)
pET-44b(+)
pETBlue-2
pET-21b(+)
pET-31b(+)
pET-44c(+)
pETcoco-1
pET-21c(+)
pET-32 Ek_LIC
pET-45b(+)
pETcoco-2
pET-21d(+)
pET-32 Ek_LIC (linearized)
pET-46 Ek_LIC
pETDuet-1
pET-22b(+)
pET-32 Xa_LIC
pET-46 Ek_LIC (linearized)
pLacI
pET-23(+)
pET-32 Xa_LIC (linearized)
pET-47b(+)
pLysE
pET-23a(+)
pET-32a(+)
pET-48b(+)
pLysS
pET-23b(+)
pET-32b(+)
pET-49b(+)
pRSF-1b
pET-23c(+)
pET-32c(+)
pET-50b(+)
pRSF-2 Ek_LIC
pET-23d(+)
pET-33b(+)
pET-51b(+)
pRSF-2 Ek_LIC (linearized)
pET-24(+)
pET-39b(+)
pET-51b(+) Ek_LIC
pRSFDuet-1

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