pET-41 Ek_LIC (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC) to express GST-tagged proteins with an enterokinase site.

Sequence Author: MilliporeSigma (Novagen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: pET & Duet Vectors (Novagen) | More Plasmid Sets
No matches
HomePlasmidspET & Duet Vectors (Novagen)pET-41 Ek_LIC (linearized)
AvrII (5801) BlpI (5750) T7 terminator DraIII (5428) PsiI (5300) AsiSI - PvuI (4727) SmaI (4601) TspMI - XmaI (4599) NruI (4384) AcuI (4073) AlwNI (3941) BssSI - BssSαI (3698) BstZ17I (3296) PflFI - Tth111I (3270) 8xHis PaeR7I - PspXI - XhoI (5844) EagI - NotI (5852) HindIII (5859) SalI (5865) Eco53kI (5874) SacI (5876) PstI - SbfI (5884) AscI (5886) StuI (5896) BsrGI (5899) EcoRI (5905) BamHI (5911) EcoRV (5921) NcoI (5924) End (5931) Start (0) enterokinase site AgeI (29) Acc65I (32) KpnI (36) BglII (39) MfeI (97) thrombin site SacII (130) 6xHis SpeI (161) SwaI (404) MscI (624) NdeI (831) XbaI (869) lac operator SgrAI (980) SphI (1136) BstAPI (1344) MluI (1661) BstEII (1842) NmeAIII (1867) PspOMI (1868) ApaI (1872) HpaI (2167) PpuMI (2531) pET-41 Ek/LIC 5931 bp
AvrII  (5801)
1 site		 C C T A G G G G A T C C
BlpI  (5750)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
DraIII  (5428)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
PsiI  (5300)
1 site		 T T A T A A A A T A T T
AsiSI  (4727)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (4727)
1 site		 C G A T C G G C T A G C
SmaI  (4601)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (4599)
1 site		 C C C G G G G G G C C C
XmaI  (4599)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
NruI  (4384)
1 site		 T C G C G A A G C G C T
AcuI  (4073)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (3941)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BssSI  (3698)
1 site		 C A C G A G G T G C T C
BssSαI  (3698)
1 site		 C A C G A G G T G C T C
BstZ17I  (3296)
1 site		 G T A T A C C A T A T G
PflFI  (3270)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3270)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PaeR7I  (5844)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (5844)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (5844)
1 site		 C T C G A G G A G C T C
EagI  (5852)
1 site		 C G G C C G G C C G G C
NotI  (5852)
1 site		 G C G G C C G C C G C C G G C G
HindIII  (5859)
1 site		 A A G C T T T T C G A A
SalI  (5865)
1 site		 G T C G A C C A G C T G
Eco53kI  (5874)
1 site		 G A G C T C C T C G A G
SacI  (5876)
1 site		 G A G C T C C T C G A G
PstI  (5884)
1 site		 C T G C A G G A C G T C
SbfI  (5884)
1 site		 C C T G C A G G G G A C G T C C
AscI  (5886)
1 site		 G G C G C G C C C C G C G C G G
StuI  (5896)
1 site		 A G G C C T T C C G G A
BsrGI  (5899)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (5905)
1 site		 G A A T T C C T T A A G
BamHI  (5911)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (5921)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NcoI  (5924)
1 site		 C C A T G G G G T A C C
End  (5931)
0 sites
Start  (0)
0 sites
AgeI  (29)
1 site		 A C C G G T T G G C C A
Acc65I  (32)
1 site		 G G T A C C C C A T G G
KpnI  (36)
1 site		 G G T A C C C C A T G G
BglII  (39)
1 site		 A G A T C T T C T A G A
MfeI  (97)
1 site		 C A A T T G G T T A A C
SacII  (130)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
SpeI  (161)
1 site		 A C T A G T T G A T C A
SwaI  (404)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
MscI  (624)
1 site		 T G G C C A A C C G G T
NdeI  (831)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
XbaI  (869)
1 site		 T C T A G A A G A T C T
SgrAI  (980)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (1136)
1 site		 G C A T G C C G T A C G
BstAPI  (1344)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
MluI  (1661)
1 site		 A C G C G T T G C G C A
BstEII  (1842)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (1867)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PspOMI  (1868)
1 site		 G G G C C C C C C G G G
ApaI  (1872)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
HpaI  (2167)
1 site		 G T T A A C C A A T T G
PpuMI  (2531)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
lacI
1311 .. 2393  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
1311 .. 2393  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
enterokinase site
2 .. 16  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
2 .. 16  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
S-Tag
47 .. 91  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from pancreatic ribonuclease A
S-Tag
47 .. 91  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from pancreatic ribonuclease A
thrombin site
107 .. 124  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
thrombin site
107 .. 124  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
6xHis
134 .. 151  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
134 .. 151  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
GST
179 .. 832  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from Schistosoma japonicum
GST
179 .. 832  =  654 bp
218 amino acids  =  25.5 kDa
Product: glutathione S-transferase from Schistosoma japonicum
KanR
4296 .. 5111  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
4296 .. 5111  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
3586 .. 4174  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3586 .. 4174  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5204 .. 5659  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5204 .. 5659  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
rop
2965 .. 3156  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
rop
2965 .. 3156  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
MCS
5844 .. 5929  =  86 bp
multiple cloning site
MCS
5844 .. 5929  =  86 bp
multiple cloning site
lacI promoter
1233 .. 1310  =  78 bp
lacI promoter
1233 .. 1310  =  78 bp
T7 terminator
5696 .. 5743  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
5696 .. 5743  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac operator
877 .. 901  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
877 .. 901  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
8xHis
5820 .. 5843  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
5820 .. 5843  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
T7 promoter
902 .. 920  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
902 .. 920  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
841 .. 846  =  6 bp
ribosome binding site
RBS
841 .. 846  =  6 bp
ribosome binding site
ORF:  2932 .. 3156  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  833 .. 1561  =  729 bp
ORF:  242 amino acids  =  25.5 kDa
ORF:  2150 .. 2413  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  1434 .. 2393  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  4296 .. 5111  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
ORF:  2565 .. 2933  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  2 .. 832  =  831 bp
ORF:  277 amino acids  =  31.5 kDa
ORF:  884 .. 1135  =  252 bp
ORF:  83 amino acids  =  8.6 kDa
ORF:  1009 .. 1272  =  264 bp
ORF:  87 amino acids  =  9.5 kDa
ORF:  2176 .. 2427  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
Click here to try SnapGene

Download pET-41 Ek_LIC (linearized).dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pACYCDuet-1
pET-24a(+)
pET-3a
pET-51b(+) Ek_LIC (linearized)
pCDF-1b
pET-24b(+)
pET-3b
pET-52b(+)
pCDF-2 Ek_LIC
pET-24c(+)
pET-3c
pET-52b(+) 3C_LIC
pCDF-2 Ek_LIC (linearized)
pET-24d(+)
pET-3d
pET-52b(+) 3C_LIC (linearized)
pCDFDuet-1
pET-25b(+)
pET-40b(+)
pET-53-DEST
pCOLA-2-DEST
pET-26b(+)
pET-41 Ek_LIC
pET-54-DEST
pCOLA-3-DEST
pET-27b(+)
pET-41 Ek_LIC (linearized)
pET-55-DEST
pCOLADuet-1
pET-28a(+)
pET-41a(+)
pET-56-DEST
pET-11a
pET-28b(+)
pET-41b(+)
pET-57-DEST
pET-11b
pET-28c(+)
pET-41c(+)
pET-58-DEST
pET-11c
pET-29a(+)
pET-42a(+)
pET-59-DEST
pET-11d
pET-29b(+)
pET-42b(+)
pET-60-DEST
pET-14b
pET-29c(+)
pET-42c(+)
pET-61-DEST
pET-15b
pET-30 Ek_LIC
pET-43.1 Ek_LIC
pET-62-DEST
pET-16b
pET-30 Ek_LIC (linearized)
pET-43.1 Ek_LIC (linearized)
pET-9a
pET-17b
pET-30 Xa_LIC
pET-43.1a(+)
pET-9b
pET-19b
pET-30 Xa_LIC (linearized)
pET-43.1b(+)
pET-9c
pET-20b(+)
pET-30a(+)
pET-43.1c(+)
pET-9d
pET-21(+)
pET-30b(+)
pET-44a(+)
pETBlue-1
pET-21a(+)
pET-30c(+)
pET-44b(+)
pETBlue-2
pET-21b(+)
pET-31b(+)
pET-44c(+)
pETcoco-1
pET-21c(+)
pET-32 Ek_LIC
pET-45b(+)
pETcoco-2
pET-21d(+)
pET-32 Ek_LIC (linearized)
pET-46 Ek_LIC
pETDuet-1
pET-22b(+)
pET-32 Xa_LIC
pET-46 Ek_LIC (linearized)
pLacI
pET-23(+)
pET-32 Xa_LIC (linearized)
pET-47b(+)
pLysE
pET-23a(+)
pET-32a(+)
pET-48b(+)
pLysS
pET-23b(+)
pET-32b(+)
pET-49b(+)
pRSF-1b
pET-23c(+)
pET-32c(+)
pET-50b(+)
pRSF-2 Ek_LIC
pET-23d(+)
pET-33b(+)
pET-51b(+)
pRSF-2 Ek_LIC (linearized)
pET-24(+)
pET-39b(+)
pET-51b(+) Ek_LIC
pRSFDuet-1

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free