pETDuet-1

Bacterial vector for the co-expression of two genes.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidspET & Duet Vectors (Novagen)pETDuet-1
EcoRI (112) BamHI (106) Bsp19I - NcoI (69) RBS XbaI (30) lac operator T7 promoter BanIII * - Bsa29I * - BseCI * - BshVI - BspDI * - BspXI * - Bsu15I * - BsuTUI * - ClaI * (5383) pET Upstream Primer (5358 .. 5373) SgrAI (5339) BbuI - PaeI - SphI (5191) BstENI - EcoNI - XagI (5126) BstAPI (4982) MluI (4658) BclI * - FbaI * - Ksp22I * (4644) BstEII - BstPI - Eco91I - EcoO65I - PspEI (4476) ApaI (4455) Bsp120I - PspOMI (4451) BstHPI - HpaI - KspAI (4156) PpuMI - Psp5II - PspPPI (3789) Bpu10I (3689) AspI - PflFI - PsyI - Tth111I (3052) BssNAI - Bst1107I - BstZ17I (3027) BspQI - LguI - PciSI - SapI (2911) BspLU11I - PciI - PscI (2794) Ecl136II - Eco53kI - EcoICRI (120) Psp124BI - SacI - SstI (122) Acc36I - BfuAI - BspMI - BveI (124) AscI - PalAI - SgsI (125) PstI - SbfI - SdaI - Sse8387I (135) SalI (137) HindIII (143) CciNI - NotI (150) AflII - BfrI - BspTI - Bst98I - BstAFI - MspCI - Vha464I (163) DuetUP2 Primer (189 .. 208) Bsp1407I - BsrGI - BstAUI - SspBI (190) DuetDOWN1 Primer (189 .. 208) T7 promoter RBS FauNDI - NdeI (298) BglII (305) MfeI - MunI (311) Eco32I - EcoRV (319) FseI - RigI (328) AsiSI - RgaI - SfaAI - SgfI (337) ZraI (344) AatII (346) Acc65I - Asp718I (348) KpnI (352) Ama87I - AvaI - BmeT110I - BsiHKCI - BsoBI - Eco88I - PaeR7I - PspXI - Sfr274I - SlaI - XhoI (354) PacI (429) AspA2I - AvrII - BlnI - XmaJI (433) BlpI - Bpu1102I - Bsp1720I - CelII (451) T7 Terminator Primer (448 .. 466) T7 terminator AdeI - DraIII (779) AanI - PsiI (904) SspI (984) AhdI - AspEI - BmeRI - DriI - Eam1105I (1189) BsaI - Bso31I - BspTNI - Eco31I (1250) BglI (1309) Acc16I - AviII - FspI - NsbI (1411) Acc113I - BmcAI - ScaI - ZrmI (1669) AlwNI - CaiI - PstNI (2385) pETDuet™-1 5420 bp
EcoRI  (112)
1 site		 G A A T T C C T T A A G
BamHI  (106)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Bsp19I  (69)
1 site		 C C A T G G G G T A C C
NcoI  (69)
1 site		 C C A T G G G G T A C C
XbaI  (30)
1 site		 T C T A G A A G A T C T
BanIII  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
Bsa29I  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BseCI  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BshVI  (5383)
1 site		 A T C G A T T A G C T A
BspDI  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BspXI  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
Bsu15I  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BsuTUI  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (5383)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
SgrAI  (5339)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BbuI  (5191)
1 site		 G C A T G C C G T A C G
PaeI  (5191)
1 site		 G C A T G C C G T A C G
SphI  (5191)
1 site		 G C A T G C C G T A C G
BstENI  (5126)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by BstENI may be hard to ligate.
Sticky ends from different BstENI sites may not be compatible.
EcoNI  (5126)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
XagI  (5126)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by XagI may be hard to ligate.
Sticky ends from different XagI sites may not be compatible.
BstAPI  (4982)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
MluI  (4658)
1 site		 A C G C G T T G C G C A
BclI  (4644)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
FbaI  (4644)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
Ksp22I  (4644)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BstEII  (4476)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstPI  (4476)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstPI sites may not be compatible.
Eco91I  (4476)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different Eco91I sites may not be compatible.
EcoO65I  (4476)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different EcoO65I sites may not be compatible.
PspEI  (4476)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different PspEI sites may not be compatible.
ApaI  (4455)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
Bsp120I  (4451)
1 site		 G G G C C C C C C G G G
PspOMI  (4451)
1 site		 G G G C C C C C C G G G
BstHPI  (4156)
1 site		 G T T A A C C A A T T G
HpaI  (4156)
1 site		 G T T A A C C A A T T G
KspAI  (4156)
1 site		 G T T A A C C A A T T G
PpuMI  (3789)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
Psp5II  (3789)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different Psp5II sites may not be compatible.
PspPPI  (3789)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PspPPI sites may not be compatible.
Bpu10I  (3689)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AspI  (3052)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by AspI may be hard to ligate.
Sticky ends from different AspI sites may not be compatible.
PflFI  (3052)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
PsyI  (3052)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PsyI may be hard to ligate.
Sticky ends from different PsyI sites may not be compatible.
Tth111I  (3052)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BssNAI  (3027)
1 site		 G T A T A C C A T A T G
Bst1107I  (3027)
1 site		 G T A T A C C A T A T G
BstZ17I  (3027)
1 site		 G T A T A C C A T A T G
BspQI  (2911)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
LguI  (2911)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different LguI sites may not be compatible.
PciSI  (2911)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different PciSI sites may not be compatible.
SapI  (2911)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspLU11I  (2794)
1 site		 A C A T G T T G T A C A
BspLU11I is inhibited by nonionic detergents.
PciI  (2794)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PscI  (2794)
1 site		 A C A T G T T G T A C A
PscI is inhibited by nonionic detergents.
Ecl136II  (120)
1 site		 G A G C T C C T C G A G
Eco53kI  (120)
1 site		 G A G C T C C T C G A G
EcoICRI  (120)
1 site		 G A G C T C C T C G A G
Psp124BI  (122)
1 site		 G A G C T C C T C G A G
SacI  (122)
1 site		 G A G C T C C T C G A G
SstI  (122)
1 site		 G A G C T C C T C G A G
Acc36I  (124)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Sticky ends from different Acc36I sites may not be compatible.
BfuAI  (124)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (124)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BveI  (124)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Cleavage may be enhanced when more than one copy of the BveI recognition sequence is present.
Sticky ends from different BveI sites may not be compatible.
After cleavage, BveI can remain bound to DNA and alter its electrophoretic mobility.
AscI  (125)
1 site		 G G C G C G C C C C G C G C G G
PalAI  (125)
1 site		 G G C G C G C C C C G C G C G G
SgsI  (125)
1 site		 G G C G C G C C C C G C G C G G
PstI  (135)
1 site		 C T G C A G G A C G T C
SbfI  (135)
1 site		 C C T G C A G G G G A C G T C C
SdaI  (135)
1 site		 C C T G C A G G G G A C G T C C
Sse8387I  (135)
1 site		 C C T G C A G G G G A C G T C C
SalI  (137)
1 site		 G T C G A C C A G C T G
HindIII  (143)
1 site		 A A G C T T T T C G A A
CciNI  (150)
1 site		 G C G G C C G C C G C C G G C G
NotI  (150)
1 site		 G C G G C C G C C G C C G G C G
AflII  (163)
1 site		 C T T A A G G A A T T C
BfrI  (163)
1 site		 C T T A A G G A A T T C
The sticky ends produced by BfrI are hard to ligate.
BspTI  (163)
1 site		 C T T A A G G A A T T C
The sticky ends produced by BspTI are hard to ligate.
Bst98I  (163)
1 site		 C T T A A G G A A T T C
The sticky ends produced by Bst98I are hard to ligate.
BstAFI  (163)
1 site		 C T T A A G G A A T T C
The sticky ends produced by BstAFI are hard to ligate.
MspCI  (163)
1 site		 C T T A A G G A A T T C
The sticky ends produced by MspCI are hard to ligate.
Vha464I  (163)
1 site		 C T T A A G G A A T T C
The sticky ends produced by Vha464I are hard to ligate.
Bsp1407I  (190)
1 site		 T G T A C A A C A T G T
BsrGI  (190)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BstAUI  (190)
1 site		 T G T A C A A C A T G T
SspBI  (190)
1 site		 T G T A C A A C A T G T
FauNDI  (298)
1 site		 C A T A T G G T A T A C
NdeI  (298)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BglII  (305)
1 site		 A G A T C T T C T A G A
MfeI  (311)
1 site		 C A A T T G G T T A A C
MunI  (311)
1 site		 C A A T T G G T T A A C
Eco32I  (319)
1 site		 G A T A T C C T A T A G
EcoRV  (319)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
FseI  (328)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
RigI  (328)
1 site		 G G C C G G C C C C G G C C G G
AsiSI  (337)
1 site		 G C G A T C G C C G C T A G C G
RgaI  (337)
1 site		 G C G A T C G C C G C T A G C G
SfaAI  (337)
1 site		 G C G A T C G C C G C T A G C G
SgfI  (337)
1 site		 G C G A T C G C C G C T A G C G
ZraI  (344)
1 site		 G A C G T C C T G C A G
AatII  (346)
1 site		 G A C G T C C T G C A G
Acc65I  (348)
1 site		 G G T A C C C C A T G G
Asp718I  (348)
1 site		 G G T A C C C C A T G G
KpnI  (352)
1 site		 G G T A C C C C A T G G
Ama87I  (354)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different Ama87I sites may not be compatible.
AvaI  (354)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (354)
1 site		 C Y C G R G G R G C Y C
BsiHKCI  (354)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsiHKCI sites may not be compatible.
BsoBI  (354)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
Eco88I  (354)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different Eco88I sites may not be compatible.
PaeR7I  (354)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (354)
1 site		 V C T C G A G B B G A G C T C V
Sfr274I  (354)
1 site		 C T C G A G G A G C T C
SlaI  (354)
1 site		 C T C G A G G A G C T C
XhoI  (354)
1 site		 C T C G A G G A G C T C
PacI  (429)
1 site		 T T A A T T A A A A T T A A T T
AspA2I  (433)
1 site		 C C T A G G G G A T C C
AvrII  (433)
1 site		 C C T A G G G G A T C C
BlnI  (433)
1 site		 C C T A G G G G A T C C
XmaJI  (433)
1 site		 C C T A G G G G A T C C
BlpI  (451)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
Bpu1102I  (451)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different Bpu1102I sites may not be compatible.
Bsp1720I  (451)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different Bsp1720I sites may not be compatible.
CelII  (451)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different CelII sites may not be compatible.
AdeI  (779)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different AdeI sites may not be compatible.
DraIII  (779)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
AanI  (904)
1 site		 T T A T A A A A T A T T
PsiI  (904)
1 site		 T T A T A A A A T A T T
SspI  (984)
1 site		 A A T A T T T T A T A A
AhdI  (1189)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AspEI  (1189)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AspEI may be hard to ligate.
Sticky ends from different AspEI sites may not be compatible.
BmeRI  (1189)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by BmeRI may be hard to ligate.
Sticky ends from different BmeRI sites may not be compatible.
DriI  (1189)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by DriI may be hard to ligate.
Sticky ends from different DriI sites may not be compatible.
Eam1105I  (1189)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by Eam1105I may be hard to ligate.
Sticky ends from different Eam1105I sites may not be compatible.
BsaI  (1250)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
Bso31I  (1250)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different Bso31I sites may not be compatible.
BspTNI  (1250)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BspTNI sites may not be compatible.
Eco31I  (1250)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different Eco31I sites may not be compatible.
BglI  (1309)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
Acc16I  (1411)
1 site		 T G C G C A A C G C G T
AviII  (1411)
1 site		 T G C G C A A C G C G T
FspI  (1411)
1 site		 T G C G C A A C G C G T
NsbI  (1411)
1 site		 T G C G C A A C G C G T
Acc113I  (1669)
1 site		 A G T A C T T C A T G A
BmcAI  (1669)
1 site		 A G T A C T T C A T G A
ScaI  (1669)
1 site		 A G T A C T T C A T G A
ZrmI  (1669)
1 site		 A G T A C T T C A T G A
AlwNI  (2385)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
CaiI  (2385)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different CaiI sites may not be compatible.
PstNI  (2385)
1 site		 C A G N N N C T G G T C N N N G A C
pET Upstream Primer
16-mer  /  63% GC
1 binding site
5358 .. 5373  =  16 annealed bases
Tm  =  57°C
DuetUP2 Primer
20-mer  /  50% GC
1 binding site
189 .. 208  =  20 annealed bases
Tm  =  57°C
DuetDOWN1 Primer
20-mer  /  50% GC
1 binding site
189 .. 208  =  20 annealed bases
Tm  =  57°C
T7 Terminator Primer
19-mer  /  53% GC
1 binding site
448 .. 466  =  19 annealed bases
Tm  =  57°C
lacI
3931 .. 5013  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
3931 .. 5013  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
AmpR
1116 .. 1976  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1116 .. 1907  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1116 .. 1976  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1908 .. 1976  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1116 .. 1976  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2150 .. 2738  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2150 .. 2738  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
546 .. 1001  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
546 .. 1001  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
rop
3168 .. 3359  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
rop
3168 .. 3359  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
MCS-2
297 .. 438  =  142 bp
multiple cloning site #2
MCS-2
297 .. 438  =  142 bp
multiple cloning site #2
MCS-1
69 .. 168  =  100 bp
multiple cloning site #1
MCS-1
69 .. 168  =  100 bp
multiple cloning site #1
AmpR promoter
1977 .. 2069  =  93 bp
AmpR promoter
1977 .. 2069  =  93 bp
lacI promoter
5014 .. 5091  =  78 bp
lacI promoter
5014 .. 5091  =  78 bp
T7 terminator
462 .. 509  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
462 .. 509  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac operator
233 .. 257  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
233 .. 257  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
3 .. 27  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
3 .. 27  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
5404 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
5404 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
214 .. 232  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
214 .. 232  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
58 .. 63  =  6 bp
ribosome binding site
RBS
58 .. 63  =  6 bp
ribosome binding site
RBS
286 .. 291  =  6 bp
ribosome binding site
RBS
286 .. 291  =  6 bp
ribosome binding site
ORF:  1246 .. 1512  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3391 .. 3759  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  5189 .. 20  =  252 bp
ORF:  83 amino acids  =  8.6 kDa
ORF:  3897 .. 4148  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  5052 .. 5315  =  264 bp
ORF:  87 amino acids  =  9.5 kDa
ORF:  4763 .. 158  =  816 bp
ORF:  271 amino acids  =  28.3 kDa
ORF:  1116 .. 1976  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3168 .. 3392  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  3911 .. 4174  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  3931 .. 4890  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
Click here to try SnapGene

Download pETDuet-1.dna file

SnapGene

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SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

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  • Annotate features on your plasmids using the curated feature database
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Individual Sequences & Maps

pACYCDuet-1
pET-24a(+)
pET-3a
pET-51b(+) Ek_LIC (linearized)
pCDF-1b
pET-24b(+)
pET-3b
pET-52b(+)
pCDF-2 Ek_LIC
pET-24c(+)
pET-3c
pET-52b(+) 3C_LIC
pCDF-2 Ek_LIC (linearized)
pET-24d(+)
pET-3d
pET-52b(+) 3C_LIC (linearized)
pCDFDuet-1
pET-25b(+)
pET-40b(+)
pET-53-DEST
pCOLA-2-DEST
pET-26b(+)
pET-41 Ek_LIC
pET-54-DEST
pCOLA-3-DEST
pET-27b(+)
pET-41 Ek_LIC (linearized)
pET-55-DEST
pCOLADuet-1
pET-28a(+)
pET-41a(+)
pET-56-DEST
pET-11a
pET-28b(+)
pET-41b(+)
pET-57-DEST
pET-11b
pET-28c(+)
pET-41c(+)
pET-58-DEST
pET-11c
pET-29a(+)
pET-42a(+)
pET-59-DEST
pET-11d
pET-29b(+)
pET-42b(+)
pET-60-DEST
pET-14b
pET-29c(+)
pET-42c(+)
pET-61-DEST
pET-15b
pET-30 Ek_LIC
pET-43.1 Ek_LIC
pET-62-DEST
pET-16b
pET-30 Ek_LIC (linearized)
pET-43.1 Ek_LIC (linearized)
pET-9a
pET-17b
pET-30 Xa_LIC
pET-43.1a(+)
pET-9b
pET-19b
pET-30 Xa_LIC (linearized)
pET-43.1b(+)
pET-9c
pET-20b(+)
pET-30a(+)
pET-43.1c(+)
pET-9d
pET-21(+)
pET-30b(+)
pET-44a(+)
pETBlue-1
pET-21a(+)
pET-30c(+)
pET-44b(+)
pETBlue-2
pET-21b(+)
pET-31b(+)
pET-44c(+)
pETcoco-1
pET-21c(+)
pET-32 Ek_LIC
pET-45b(+)
pETcoco-2
pET-21d(+)
pET-32 Ek_LIC (linearized)
pET-46 Ek_LIC
pETDuet-1
pET-22b(+)
pET-32 Xa_LIC
pET-46 Ek_LIC (linearized)
pLacI
pET-23(+)
pET-32 Xa_LIC (linearized)
pET-47b(+)
pLysE
pET-23a(+)
pET-32a(+)
pET-48b(+)
pLysS
pET-23b(+)
pET-32b(+)
pET-49b(+)
pRSF-1b
pET-23c(+)
pET-32c(+)
pET-50b(+)
pRSF-2 Ek_LIC
pET-23d(+)
pET-33b(+)
pET-51b(+)
pRSF-2 Ek_LIC (linearized)
pET-24(+)
pET-39b(+)
pET-51b(+) Ek_LIC
pRSFDuet-1

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

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