pLysE

Plasmid for expressing high levels of T7 lysozyme, an inhibitor of T7 RNA polymerase

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidspET & Duet Vectors (Novagen)pLysE
BsrDI (4874) NcoI (4586) ScaI (4474) BaeGI - Bme1580I (4424) DrdI - PflFI - Tth111I (4343) Bsu36I (4234) BclI * (4183) BsaBI * (4087) AhdI (3850) AvaI - BsoBI (3561) BfuAI - BspMI (3190) BstAPI (3187) NruI (3110) PspFI (3089) BseYI (3085) EagI (3075) SalI (2787) EcoNI (2762) SphI (2702) PsiI (2450) EcoRI (1) BstZ17I (596) XmnI (639) PfoI * (788) SacII (835) XbaI (1424) EarI (1452) BspDI - ClaI (1518) HindIII (1523) EcoRV (1681) KflI (1876) BsrGI (1926) PmeI (1935) BciVI (2047) AflII (2323) BsaI (2388) pLysE 4886 bp
BsrDI  (4874)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
NcoI  (4586)
1 site		 C C A T G G G G T A C C
ScaI  (4474)
1 site		 A G T A C T T C A T G A
BaeGI  (4424)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (4424)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
DrdI  (4343)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PflFI  (4343)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4343)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
Bsu36I  (4234)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BclI  (4183)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsaBI  (4087)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
AhdI  (3850)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AvaI  (3561)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (3561)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BfuAI  (3190)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3190)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BstAPI  (3187)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
NruI  (3110)
1 site		 T C G C G A A G C G C T
PspFI  (3089)
1 site		 C C C A G C G G G T C G
BseYI  (3085)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
EagI  (3075)
1 site		 C G G C C G G C C G G C
SalI  (2787)
1 site		 G T C G A C C A G C T G
EcoNI  (2762)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SphI  (2702)
1 site		 G C A T G C C G T A C G
PsiI  (2450)
1 site		 T T A T A A A A T A T T
EcoRI  (1)
1 site		 G A A T T C C T T A A G
BstZ17I  (596)
1 site		 G T A T A C C A T A T G
XmnI  (639)
1 site		 G A A N N N N T T C C T T N N N N A A G
PfoI  (788)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SacII  (835)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
XbaI  (1424)
1 site		 T C T A G A A G A T C T
EarI  (1452)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BspDI  (1518)
1 site		 A T C G A T T A G C T A
ClaI  (1518)
1 site		 A T C G A T T A G C T A
HindIII  (1523)
1 site		 A A G C T T T T C G A A
EcoRV  (1681)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
KflI  (1876)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
BsrGI  (1926)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
PmeI  (1935)
1 site		 G T T T A A A C C A A A T T T G
BciVI  (2047)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
AflII  (2323)
1 site		 C T T A A G G A A T T C
BsaI  (2388)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
CmR
4446 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
4446 .. 219  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
p15A ori
848 .. 1392  =  545 bp
Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.
p15A ori
848 .. 1392  =  545 bp
Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.
T7 lysozyme
1918 .. 2373  =  456 bp
151 amino acids  =  16.9 kDa
Product: lysozyme from bacteriophage T7
inhibitor of T7 RNA polymerase
T7 lysozyme
1918 .. 2373  =  456 bp
151 amino acids  =  16.9 kDa
Product: lysozyme from bacteriophage T7
inhibitor of T7 RNA polymerase
cat promoter
220 .. 322  =  103 bp
promoter of the E. coli cat gene
cat promoter
220 .. 322  =  103 bp
promoter of the E. coli cat gene
tet promoter
1504 .. 1532  =  29 bp
3 segments
   Segment 1:  -35  
   1504 .. 1509  =  6 bp
promoter for tetracycline efflux protein gene
tet promoter
1504 .. 1532  =  29 bp
3 segments
   Segment 2:  
   1510 .. 1526  =  17 bp
promoter for tetracycline efflux protein gene
tet promoter
1504 .. 1532  =  29 bp
3 segments
   Segment 3:  -10  
   1527 .. 1532  =  6 bp
promoter for tetracycline efflux protein gene
tet promoter
1504 .. 1532  =  29 bp
3 segments
promoter for tetracycline efflux protein gene
T7 Φ3.8 promoter
2371 .. 2404  =  34 bp
weak promoter for bacteriophage T7 RNA polymerase
T7 Φ3.8 promoter
2371 .. 2404  =  34 bp
weak promoter for bacteriophage T7 RNA polymerase
ORF:  763 .. 1041  =  279 bp
ORF:  92 amino acids  =  10.4 kDa
ORF:  1918 .. 2373  =  456 bp
ORF:  151 amino acids  =  16.9 kDa
ORF:  2437 .. 2778  =  342 bp
ORF:  113 amino acids  =  13.1 kDa
ORF:  3316 .. 3654  =  339 bp
ORF:  112 amino acids  =  12.6 kDa
ORF:  3862 .. 4149  =  288 bp
ORF:  95 amino acids  =  10.0 kDa
ORF:  1580 .. 1918  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
ORF:  2504 .. 3412  =  909 bp
ORF:  302 amino acids  =  31.6 kDa
ORF:  3651 .. 3875  =  225 bp
ORF:  74 amino acids  =  8.2 kDa
ORF:  2387 .. 2701  =  315 bp
ORF:  104 amino acids  =  10.9 kDa
ORF:  2759 .. 3217  =  459 bp
ORF:  152 amino acids  =  16.7 kDa
ORF:  4446 .. 219  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  730 .. 1002  =  273 bp
ORF:  90 amino acids  =  9.7 kDa
ORF:  2575 .. 2925  =  351 bp
ORF:  116 amino acids  =  12.2 kDa
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Download pLysE.dna file

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Individual Sequences & Maps

pACYCDuet-1
pET-24a(+)
pET-3a
pET-51b(+) Ek_LIC (linearized)
pCDF-1b
pET-24b(+)
pET-3b
pET-52b(+)
pCDF-2 Ek_LIC
pET-24c(+)
pET-3c
pET-52b(+) 3C_LIC
pCDF-2 Ek_LIC (linearized)
pET-24d(+)
pET-3d
pET-52b(+) 3C_LIC (linearized)
pCDFDuet-1
pET-25b(+)
pET-40b(+)
pET-53-DEST
pCOLA-2-DEST
pET-26b(+)
pET-41 Ek_LIC
pET-54-DEST
pCOLA-3-DEST
pET-27b(+)
pET-41 Ek_LIC (linearized)
pET-55-DEST
pCOLADuet-1
pET-28a(+)
pET-41a(+)
pET-56-DEST
pET-11a
pET-28b(+)
pET-41b(+)
pET-57-DEST
pET-11b
pET-28c(+)
pET-41c(+)
pET-58-DEST
pET-11c
pET-29a(+)
pET-42a(+)
pET-59-DEST
pET-11d
pET-29b(+)
pET-42b(+)
pET-60-DEST
pET-14b
pET-29c(+)
pET-42c(+)
pET-61-DEST
pET-15b
pET-30 Ek_LIC
pET-43.1 Ek_LIC
pET-62-DEST
pET-16b
pET-30 Ek_LIC (linearized)
pET-43.1 Ek_LIC (linearized)
pET-9a
pET-17b
pET-30 Xa_LIC
pET-43.1a(+)
pET-9b
pET-19b
pET-30 Xa_LIC (linearized)
pET-43.1b(+)
pET-9c
pET-20b(+)
pET-30a(+)
pET-43.1c(+)
pET-9d
pET-21(+)
pET-30b(+)
pET-44a(+)
pETBlue-1
pET-21a(+)
pET-30c(+)
pET-44b(+)
pETBlue-2
pET-21b(+)
pET-31b(+)
pET-44c(+)
pETcoco-1
pET-21c(+)
pET-32 Ek_LIC
pET-45b(+)
pETcoco-2
pET-21d(+)
pET-32 Ek_LIC (linearized)
pET-46 Ek_LIC
pETDuet-1
pET-22b(+)
pET-32 Xa_LIC
pET-46 Ek_LIC (linearized)
pLacI
pET-23(+)
pET-32 Xa_LIC (linearized)
pET-47b(+)
pLysE
pET-23a(+)
pET-32a(+)
pET-48b(+)
pLysS
pET-23b(+)
pET-32b(+)
pET-49b(+)
pRSF-1b
pET-23c(+)
pET-32c(+)
pET-50b(+)
pRSF-2 Ek_LIC
pET-23d(+)
pET-33b(+)
pET-51b(+)
pRSF-2 Ek_LIC (linearized)
pET-24(+)
pET-39b(+)
pET-51b(+) Ek_LIC
pRSFDuet-1

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