pET-9b

Bacterial expression vector with a BamHI cloning site. For alternative reading frames, use pET-9a or pET-9c.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidspET & Duet Vectors (Novagen)pET-9b
EcoRI (4338) AsiSI - PvuI (3953) SspI (3878) SmaI (3827) TspMI - XmaI (3825) PaeR7I - PspXI - XhoI (3551) AcuI (3299) AlwNI (3167) BciVI (2954) BssSI - BssSαI (2924) AflIII - PciI (2751) BsrBI (2684) BspQI - SapI (2635) TatI (2555) BstZ17I (2522) BsaAI (2503) PflFI - Tth111I (2496) PfoI (2393) PvuII (2342) BlpI (458) BamHI (510) NdeI (549) RBS XbaI (587) BsaI (612) T7 promoter BglII (645) SgrAI (686) SphI (842) SalI (927) HincII (929) PshAI (992) EagI (1215) BstAPI (1327) BfuAI - BspMI (1330) MscI * (1722) BsgI * (1911) XmnI (2309) pET-9b 4340 bp
EcoRI  (4338)
1 site		 G A A T T C C T T A A G
AsiSI  (3953)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (3953)
1 site		 C G A T C G G C T A G C
SspI  (3878)
1 site		 A A T A T T T T A T A A
SmaI  (3827)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (3825)
1 site		 C C C G G G G G G C C C
XmaI  (3825)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
PaeR7I  (3551)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3551)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (3551)
1 site		 C T C G A G G A G C T C
AcuI  (3299)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (3167)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BciVI  (2954)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSI  (2924)
1 site		 C A C G A G G T G C T C
BssSαI  (2924)
1 site		 C A C G A G G T G C T C
AflIII  (2751)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (2751)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BsrBI  (2684)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
BspQI  (2635)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (2635)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
TatI  (2555)
1 site		 W G T A C W W C A T G W
BstZ17I  (2522)
1 site		 G T A T A C C A T A T G
BsaAI  (2503)
1 site		 Y A C G T R R T G C A Y
PflFI  (2496)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2496)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PfoI  (2393)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
PvuII  (2342)
1 site		 C A G C T G G T C G A C
BlpI  (458)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BamHI  (510)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NdeI  (549)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
XbaI  (587)
1 site		 T C T A G A A G A T C T
BsaI  (612)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BglII  (645)
1 site		 A G A T C T T C T A G A
SgrAI  (686)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (842)
1 site		 G C A T G C C G T A C G
SalI  (927)
1 site		 G T C G A C C A G C T G
HincII  (929)
1 site		 G T Y R A C C A R Y T G
PshAI  (992)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
EagI  (1215)
1 site		 C G G C C G G C C G G C
BstAPI  (1327)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BfuAI  (1330)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1330)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
MscI  (1722)
1 site		 T G G C C A A C C G G T
* Blocked by Dcm methylation.
BsgI  (1911)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
* Blocked by EcoKI methylation.
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XmnI  (2309)
1 site		 G A A N N N N T T C C T T N N N N A A G
KanR
3522 .. 4337  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
3522 .. 4337  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
2812 .. 3400  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2812 .. 3400  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
rop
2191 .. 2382  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
rop
2191 .. 2382  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein
T7 terminator
404 .. 451  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
404 .. 451  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 tag
518 .. 550  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
T7 tag
518 .. 550  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
T7 promoter
612 .. 630  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
612 .. 630  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
559 .. 564  =  6 bp
ribosome binding site
RBS
559 .. 564  =  6 bp
ribosome binding site
ORF:  1456 .. 1794  =  339 bp
ORF:  112 amino acids  =  12.6 kDa
ORF:  2158 .. 2382  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  86 .. 451  =  366 bp
ORF:  121 amino acids  =  12.9 kDa
ORF:  767 .. 1552  =  786 bp
ORF:  261 amino acids  =  27.5 kDa
ORF:  3522 .. 4337  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
ORF:  1791 .. 2159  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  572 .. 841  =  270 bp
ORF:  89 amino acids  =  9.3 kDa
ORF:  899 .. 1357  =  459 bp
ORF:  152 amino acids  =  16.7 kDa
ORF:  715 .. 1065  =  351 bp
ORF:  116 amino acids  =  12.2 kDa
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Download pET-9b.dna file

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Individual Sequences & Maps

pACYCDuet-1
pET-24a(+)
pET-3a
pET-51b(+) Ek_LIC (linearized)
pCDF-1b
pET-24b(+)
pET-3b
pET-52b(+)
pCDF-2 Ek_LIC
pET-24c(+)
pET-3c
pET-52b(+) 3C_LIC
pCDF-2 Ek_LIC (linearized)
pET-24d(+)
pET-3d
pET-52b(+) 3C_LIC (linearized)
pCDFDuet-1
pET-25b(+)
pET-40b(+)
pET-53-DEST
pCOLA-2-DEST
pET-26b(+)
pET-41 Ek_LIC
pET-54-DEST
pCOLA-3-DEST
pET-27b(+)
pET-41 Ek_LIC (linearized)
pET-55-DEST
pCOLADuet-1
pET-28a(+)
pET-41a(+)
pET-56-DEST
pET-11a
pET-28b(+)
pET-41b(+)
pET-57-DEST
pET-11b
pET-28c(+)
pET-41c(+)
pET-58-DEST
pET-11c
pET-29a(+)
pET-42a(+)
pET-59-DEST
pET-11d
pET-29b(+)
pET-42b(+)
pET-60-DEST
pET-14b
pET-29c(+)
pET-42c(+)
pET-61-DEST
pET-15b
pET-30 Ek_LIC
pET-43.1 Ek_LIC
pET-62-DEST
pET-16b
pET-30 Ek_LIC (linearized)
pET-43.1 Ek_LIC (linearized)
pET-9a
pET-17b
pET-30 Xa_LIC
pET-43.1a(+)
pET-9b
pET-19b
pET-30 Xa_LIC (linearized)
pET-43.1b(+)
pET-9c
pET-20b(+)
pET-30a(+)
pET-43.1c(+)
pET-9d
pET-21(+)
pET-30b(+)
pET-44a(+)
pETBlue-1
pET-21a(+)
pET-30c(+)
pET-44b(+)
pETBlue-2
pET-21b(+)
pET-31b(+)
pET-44c(+)
pETcoco-1
pET-21c(+)
pET-32 Ek_LIC
pET-45b(+)
pETcoco-2
pET-21d(+)
pET-32 Ek_LIC (linearized)
pET-46 Ek_LIC
pETDuet-1
pET-22b(+)
pET-32 Xa_LIC
pET-46 Ek_LIC (linearized)
pLacI
pET-23(+)
pET-32 Xa_LIC (linearized)
pET-47b(+)
pLysE
pET-23a(+)
pET-32a(+)
pET-48b(+)
pLysS
pET-23b(+)
pET-32b(+)
pET-49b(+)
pRSF-1b
pET-23c(+)
pET-32c(+)
pET-50b(+)
pRSF-2 Ek_LIC
pET-23d(+)
pET-33b(+)
pET-51b(+)
pRSF-2 Ek_LIC (linearized)
pET-24(+)
pET-39b(+)
pET-51b(+) Ek_LIC
pRSFDuet-1

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