YCp50-poly

Derivative of yeast centromeric vector YCp50 containing the pUC19 polylinker.
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HomePlasmidsYeast PlasmidsYCp50-poly
AatII (6990) ZraI (6988) PvuI (6438) AhdI (6068) BsaAI (4929) PfoI (4819) BglII (4527) BmtI (4346) NheI (4342) PaeR7I - XhoI (3812) MfeI (3745) HpaI (3682) EcoRI (1) Eco53kI (9) SacI (11) Acc65I (13) KpnI (17) BamHI (22) XbaI (28) MCS SphI (50) HindIII (52) M13 rev lac operator lac promoter PshAI (300) EagI (523) NruI (558) KasI (789) NarI (790) SfoI (791) PluTI (793) XcmI (1370) EcoRV (1430) NcoI (1447) BstBI (1507) PspOMI * (1616) ApaI * (1620) NsiI (2069) MscI * (2208) FspAI (2218) BspEI * (2426) PaqCI (2818) SpeI (2875) YCp50-poly 7060 bp
AatII  (6990)
1 site		 G A C G T C C T G C A G
ZraI  (6988)
1 site		 G A C G T C C T G C A G
PvuI  (6438)
1 site		 C G A T C G G C T A G C
AhdI  (6068)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaAI  (4929)
1 site		 Y A C G T R R T G C A Y
PfoI  (4819)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
BglII  (4527)
1 site		 A G A T C T T C T A G A
BmtI  (4346)
1 site		 G C T A G C C G A T C G
NheI  (4342)
1 site		 G C T A G C C G A T C G
PaeR7I  (3812)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (3812)
1 site		 C T C G A G G A G C T C
MfeI  (3745)
1 site		 C A A T T G G T T A A C
HpaI  (3682)
1 site		 G T T A A C C A A T T G
EcoRI  (1)
1 site		 G A A T T C C T T A A G
Eco53kI  (9)
1 site		 G A G C T C C T C G A G
SacI  (11)
1 site		 G A G C T C C T C G A G
Acc65I  (13)
1 site		 G G T A C C C C A T G G
KpnI  (17)
1 site		 G G T A C C C C A T G G
BamHI  (22)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (28)
1 site		 T C T A G A A G A T C T
SphI  (50)
1 site		 G C A T G C C G T A C G
HindIII  (52)
1 site		 A A G C T T T T C G A A
PshAI  (300)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
EagI  (523)
1 site		 C G G C C G G C C G G C
NruI  (558)
1 site		 T C G C G A A G C G C T
KasI  (789)
1 site		 G G C G C C C C G C G G
NarI  (790)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (791)
1 site		 G G C G C C C C G C G G
PluTI  (793)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
XcmI  (1370)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoRV  (1430)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NcoI  (1447)
1 site		 C C A T G G G G T A C C
BstBI  (1507)
1 site		 T T C G A A A A G C T T
PspOMI  (1616)
1 site		 G G G C C C C C C G G G
* Blocked by Dcm methylation.
ApaI  (1620)
1 site		 G G G C C C C C C G G G
* Blocked by Dcm methylation.
ApaI can be used between 25°C and 37°C.
NsiI  (2069)
1 site		 A T G C A T T A C G T A
MscI  (2208)
1 site		 T G G C C A A C C G G T
* Blocked by Dcm methylation.
FspAI  (2218)
1 site		 R T G C G C A Y Y A C G C G T R
BspEI  (2426)
1 site		 T C C G G A A G G C C T
* Blocked by Dam methylation.
PaqCI  (2818)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SpeI  (2875)
1 site		 A C T A G T T G A T C A
CEN/ARS
3390 .. 4768  =  1379 bp
S. cerevisiae CEN4 centromere fused to the autonomously replicating sequence ARS1/ARS416
CEN/ARS
3390 .. 4768  =  1379 bp
S. cerevisiae CEN4 centromere fused to the autonomously replicating sequence ARS1/ARS416
AmpR
5995 .. 6855  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   5995 .. 6786  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5995 .. 6855  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   6787 .. 6855  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5995 .. 6855  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
URA3
1242 .. 2045  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
URA3
1242 .. 2045  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
ori
5236 .. 5824  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
5236 .. 5824  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
URA3 promoter
1026 .. 1241  =  216 bp
URA3 promoter
1026 .. 1241  =  216 bp
AmpR promoter
6856 .. 6960  =  105 bp
AmpR promoter
6856 .. 6960  =  105 bp
MCS
1 .. 57  =  57 bp
pUC19 multiple cloning site
MCS
1 .. 57  =  57 bp
pUC19 multiple cloning site
lac promoter
118 .. 148  =  31 bp
3 segments
   Segment 3:  -10  
   118 .. 124  =  7 bp
promoter for the E. coli lac operon
lac promoter
118 .. 148  =  31 bp
3 segments
   Segment 2:  
   125 .. 142  =  18 bp
promoter for the E. coli lac operon
lac promoter
118 .. 148  =  31 bp
3 segments
   Segment 1:  -35  
   143 .. 148  =  6 bp
promoter for the E. coli lac operon
lac promoter
118 .. 148  =  31 bp
3 segments
promoter for the E. coli lac operon
M13 rev
70 .. 86  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
70 .. 86  =  17 bp
common sequencing primer, one of multiple similar variants
lac operator
94 .. 110  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
94 .. 110  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  3754 .. 3978  =  225 bp
ORF:  74 amino acids  =  8.0 kDa
ORF:  764 .. 1087  =  324 bp
ORF:  107 amino acids  =  12.1 kDa
ORF:  6125 .. 6391  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  243 .. 860  =  618 bp
ORF:  205 amino acids  =  21.8 kDa
ORF:  1242 .. 2045  =  804 bp
ORF:  267 amino acids  =  29.3 kDa
ORF:  2580 .. 2879  =  300 bp
ORF:  99 amino acids  =  12.1 kDa
ORF:  4521 .. 4808  =  288 bp
ORF:  95 amino acids  =  10.7 kDa
ORF:  1277 .. 1753  =  477 bp
ORF:  158 amino acids  =  16.8 kDa
ORF:  5995 .. 6855  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  177 .. 665  =  489 bp
ORF:  162 amino acids  =  17.6 kDa
ORF:  2277 .. 2675  =  399 bp
ORF:  132 amino acids  =  15.3 kDa
ORF:  3717 .. 4049  =  333 bp
ORF:  110 amino acids  =  12.7 kDa
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Download YCp50-poly.dna file

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Individual Sequences & Maps

bleMX6
pFA6a-kanMX6
pPIC6(alpha) B
pYES2.1-E
hphMX6
pFA6a-kanMX6-PGAL1
pPIC6(alpha) C
pYES2 CT
kanMX
pFA6a-kanMX6-PGAL1-3HA
pPIC9
pYES2 NT A
kanMX2
pFA6a-kanMX6-PGAL1-GFP
pPIC9K
pYES2 NT B
kanMX4
pFA6a-kanMX6-PGAL1-GST
pPICZ A
pYES2 NT C
kanMX6
pFA6a-natMX6
pPICZ B
pYES3 CT
natMX6
pFA6a-TRP1
pPICZ C
pYES6 CT
pAbAi
pFA6a-TRP1-PGAL1
pPICZ(alpha) A
pYM25
pAD-GAL4
pFA6a-TRP1-PGAL1-3HA
pPICZ(alpha) B
pYM26
pAG25
pFA6a-TRP1-PGAL1-GFP
pPICZ(alpha) C
pYM44
pAG29
pFA6a-TRP1-PGAL1-GST
pPink-HC
YCp50
pAG32
pFLD
pPink-LC
YCp50-poly
pAO815
pFLD(alpha)
pPINK(alpha)-HC
YCplac111
patMX4
pGAD-C1
pRS303
YCplac22
pAUR101
pGAD-C2
pRS304
YCplac33
pAUR112
pGAD-C3
pRS305
YEp13
pAUR123
pGADT7 AD
pRS306
YEp24
pAUR224
pGADT7-Rec
pRS313
YEp351
pBridge
pGADT7-Rec (linearized)
pRS314
YEp352
pDH25
pGAPZ A
pRS315
YEp353
pEG202
pGAPZ B
pRS316
YEp354
pESC-HIS
pGAPZ C
pRS403
YEp355
pESC-LEU
pGAPZ(alpha) A
pRS404
YEp356
pESC-TRP
pGAPZ(alpha) B
pRS405
YEp356R
pESC-URA
pGAPZ(alpha) C
pRS406
YEp357
pFA6-kanMX4
pGBD-C1
pRS413
YEp357R
pFA6a
pGBD-C2
pRS414
YEp358
pFA6a-12Pk-kanMX6
pGBD-C3
pRS415
YEp358R
pFA6a-13Myc-His3MX6
pGBDU-C1
pRS416
YEp363
pFA6a-13Myc-kanMX6
pGBDU-C2
pRS423
YEp364
pFA6a-13Myc-TRP1
pGBDU-C3
pRS424
YEp365
pFA6a-3HA-His3MX6
pGBKT7
pRS425
YEp366
pFA6a-3HA-kanMX6
pGilda
pRS426
YEp366R
pFA6a-3HA-TRP1
pHIL-D2
pSpot5
YEp367
pFA6a-5FLAG-kanMX6
pHIL-S1
pSpot6
YEp367R
pFA6a-bleMX6
pJG4-5
pSpot7
YEp368
pFA6a-GFP(S65T)-His3MX6
pKLAC1
pSpot8
YEp368R
pFA6a-GFP(S65T)-kanMX6
pKLAC2
pTEF1 Zeo
YEplac112
pFA6a-GFP(S65T)-TRP1
pOAD
pUCATPH
YEplac181
pFA6a-GST-His3MX6
pOBD2
pY-secSUMOstar
YEplac195
pFA6a-GST-kanMX6
pP-secSUMO3
pY-SUMOstar
YIp351
pFA6a-GST-TRP1
pPIC3
pYC2-E
YIp352
pFA6a-His3MX6
pPIC3.5
pYC2 CT
YIp5
pFA6a-His3MX6-PGAL1
pPIC3.5K
pYC2 NT A
YIplac128
pFA6a-His3MX6-PGAL1-3HA
pPIC6 A
pYC2 NT B
YIplac204
pFA6a-His3MX6-PGAL1-GFP
pPIC6 B
pYC2 NT C
YIplac211
pFA6a-His3MX6-PGAL1-GST
pPIC6 C
pYC6 CT
YRp7
pFA6a-hphMX6
pPIC6(alpha) A
pYES2
alpha-factor secretion signal

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Structural Genomics Vectors

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