pGBDU-C1

Yeast two-hybrid "bait" vector with a URA3 marker for fusing a gene to the GAL4 DNA binding domain. For other reading frames, use pGBDU-C2 or pGBDU-C3.
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HomePlasmidsYeast PlasmidspGBDU-C1
BsaAI - SnaBI (5355) BmgBI (4913) PfoI (4692) AatII (4581) ZraI (4579) TsoI (4058) PvuI (4029) NmeAIII (3807) BglI (3779) AhdI (3659) BanI (3607) PaeR7I - XhoI (651) HpaI (711) BsrGI (724) EcoRI (878) TspMI - XmaI (884) SmaI (886) BamHI (890) BspDI - ClaI (897) SalI (902) PstI (912) BglII (914) BseRI (1020) MscI (1050) BstXI (1182) NdeI (1626) PpuMI (1862) EcoRV (1897) NcoI (1914) BsmI (2071) PspOMI * (2083) ApaI * (2087) StuI (2145) Bpu10I (2439) pGBDU-C1 5997 bp
BsaAI  (5355)
1 site		 Y A C G T R R T G C A Y
SnaBI  (5355)
1 site		 T A C G T A A T G C A T
BmgBI  (4913)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PfoI  (4692)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
AatII  (4581)
1 site		 G A C G T C C T G C A G
ZraI  (4579)
1 site		 G A C G T C C T G C A G
TsoI  (4058)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuI  (4029)
1 site		 C G A T C G G C T A G C
NmeAIII  (3807)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (3779)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
AhdI  (3659)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BanI  (3607)
1 site		 G G Y R C C C C R Y G G
Sticky ends from different BanI sites may not be compatible.
PaeR7I  (651)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (651)
1 site		 C T C G A G G A G C T C
HpaI  (711)
1 site		 G T T A A C C A A T T G
BsrGI  (724)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (878)
1 site		 G A A T T C C T T A A G
TspMI  (884)
1 site		 C C C G G G G G G C C C
XmaI  (884)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (886)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (890)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BspDI  (897)
1 site		 A T C G A T T A G C T A
ClaI  (897)
1 site		 A T C G A T T A G C T A
SalI  (902)
1 site		 G T C G A C C A G C T G
PstI  (912)
1 site		 C T G C A G G A C G T C
BglII  (914)
1 site		 A G A T C T T C T A G A
BseRI  (1020)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
MscI  (1050)
1 site		 T G G C C A A C C G G T
BstXI  (1182)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
NdeI  (1626)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PpuMI  (1862)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
EcoRV  (1897)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NcoI  (1914)
1 site		 C C A T G G G G T A C C
BsmI  (2071)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
PspOMI  (2083)
1 site		 G G G C C C C C C G G G
* Blocked by Dcm methylation.
ApaI  (2087)
1 site		 G G G C C C C C C G G G
* Blocked by Dcm methylation.
ApaI can be used between 25°C and 37°C.
StuI  (2145)
1 site		 A G G C C T T C C G G A
Bpu10I  (2439)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
2μ ori
4833 .. 5997  =  1165 bp
yeast 2μ plasmid origin of replication
2μ ori
4833 .. 5997  =  1165 bp
yeast 2μ plasmid origin of replication
AmpR
3586 .. 4446  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3586 .. 4377  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3586 .. 4446  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4378 .. 4446  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3586 .. 4446  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
URA3
1709 .. 2512  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
URA3
1709 .. 2512  =  804 bp
267 amino acids  =  29.3 kDa
Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis
yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)
ori
2827 .. 3415  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
2827 .. 3415  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
GAL4 DNA binding domain
434 .. 874  =  441 bp
147 amino acids  =  16.9 kDa
Product: DNA binding domain of the GAL4 transcriptional activator
GAL4 DNA binding domain
434 .. 874  =  441 bp
147 amino acids  =  16.9 kDa
Product: DNA binding domain of the GAL4 transcriptional activator
ADH1 promoter
5 .. 406  =  402 bp
promoter for alcohol dehydrogenase 1
ADH1 promoter
5 .. 406  =  402 bp
promoter for alcohol dehydrogenase 1
URA3 promoter
1492 .. 1708  =  217 bp
URA3 promoter
1492 .. 1708  =  217 bp
ADH1 terminator
1294 .. 1481  =  188 bp
transcription terminator for alcohol dehydrogenase 1
ADH1 terminator
1294 .. 1481  =  188 bp
transcription terminator for alcohol dehydrogenase 1
AmpR promoter
4447 .. 4551  =  105 bp
AmpR promoter
4447 .. 4551  =  105 bp
MCS
878 .. 919  =  42 bp
multiple cloning site
MCS
878 .. 919  =  42 bp
multiple cloning site
ORF:  4930 .. 5196  =  267 bp
ORF:  88 amino acids  =  10.3 kDa
ORF:  434 .. 937  =  504 bp
ORF:  167 amino acids  =  19.2 kDa
ORF:  1709 .. 2512  =  804 bp
ORF:  267 amino acids  =  29.3 kDa
ORF:  3716 .. 3982  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1744 .. 2220  =  477 bp
ORF:  158 amino acids  =  16.8 kDa
ORF:  3586 .. 4446  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1517 .. 1747  =  231 bp
ORF:  76 amino acids  =  8.9 kDa
ORF:  5672 .. 5962  =  291 bp
ORF:  96 amino acids  =  11.5 kDa
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Individual Sequences & Maps

bleMX6
pFA6a-kanMX6
pPIC6(alpha) B
pYES2.1-E
hphMX6
pFA6a-kanMX6-PGAL1
pPIC6(alpha) C
pYES2 CT
kanMX
pFA6a-kanMX6-PGAL1-3HA
pPIC9
pYES2 NT A
kanMX2
pFA6a-kanMX6-PGAL1-GFP
pPIC9K
pYES2 NT B
kanMX4
pFA6a-kanMX6-PGAL1-GST
pPICZ A
pYES2 NT C
kanMX6
pFA6a-natMX6
pPICZ B
pYES3 CT
natMX6
pFA6a-TRP1
pPICZ C
pYES6 CT
pAbAi
pFA6a-TRP1-PGAL1
pPICZ(alpha) A
pYM25
pAD-GAL4
pFA6a-TRP1-PGAL1-3HA
pPICZ(alpha) B
pYM26
pAG25
pFA6a-TRP1-PGAL1-GFP
pPICZ(alpha) C
pYM44
pAG29
pFA6a-TRP1-PGAL1-GST
pPink-HC
YCp50
pAG32
pFLD
pPink-LC
YCp50-poly
pAO815
pFLD(alpha)
pPINK(alpha)-HC
YCplac111
patMX4
pGAD-C1
pRS303
YCplac22
pAUR101
pGAD-C2
pRS304
YCplac33
pAUR112
pGAD-C3
pRS305
YEp13
pAUR123
pGADT7 AD
pRS306
YEp24
pAUR224
pGADT7-Rec
pRS313
YEp351
pBridge
pGADT7-Rec (linearized)
pRS314
YEp352
pDH25
pGAPZ A
pRS315
YEp353
pEG202
pGAPZ B
pRS316
YEp354
pESC-HIS
pGAPZ C
pRS403
YEp355
pESC-LEU
pGAPZ(alpha) A
pRS404
YEp356
pESC-TRP
pGAPZ(alpha) B
pRS405
YEp356R
pESC-URA
pGAPZ(alpha) C
pRS406
YEp357
pFA6-kanMX4
pGBD-C1
pRS413
YEp357R
pFA6a
pGBD-C2
pRS414
YEp358
pFA6a-12Pk-kanMX6
pGBD-C3
pRS415
YEp358R
pFA6a-13Myc-His3MX6
pGBDU-C1
pRS416
YEp363
pFA6a-13Myc-kanMX6
pGBDU-C2
pRS423
YEp364
pFA6a-13Myc-TRP1
pGBDU-C3
pRS424
YEp365
pFA6a-3HA-His3MX6
pGBKT7
pRS425
YEp366
pFA6a-3HA-kanMX6
pGilda
pRS426
YEp366R
pFA6a-3HA-TRP1
pHIL-D2
pSpot5
YEp367
pFA6a-5FLAG-kanMX6
pHIL-S1
pSpot6
YEp367R
pFA6a-bleMX6
pJG4-5
pSpot7
YEp368
pFA6a-GFP(S65T)-His3MX6
pKLAC1
pSpot8
YEp368R
pFA6a-GFP(S65T)-kanMX6
pKLAC2
pTEF1 Zeo
YEplac112
pFA6a-GFP(S65T)-TRP1
pOAD
pUCATPH
YEplac181
pFA6a-GST-His3MX6
pOBD2
pY-secSUMOstar
YEplac195
pFA6a-GST-kanMX6
pP-secSUMO3
pY-SUMOstar
YIp351
pFA6a-GST-TRP1
pPIC3
pYC2-E
YIp352
pFA6a-His3MX6
pPIC3.5
pYC2 CT
YIp5
pFA6a-His3MX6-PGAL1
pPIC3.5K
pYC2 NT A
YIplac128
pFA6a-His3MX6-PGAL1-3HA
pPIC6 A
pYC2 NT B
YIplac204
pFA6a-His3MX6-PGAL1-GFP
pPIC6 B
pYC2 NT C
YIplac211
pFA6a-His3MX6-PGAL1-GST
pPIC6 C
pYC6 CT
YRp7
pFA6a-hphMX6
pPIC6(alpha) A
pYES2
alpha-factor secretion signal

Plasmid Sets

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Yeast Plasmids
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Mammalian Expression Vectors
Structural Genomics Vectors

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