pHIL-D2

Pichia pastoris HIS4 vector for methanol-inducible intracellular expression of a protein.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Yeast Plasmids | More Plasmid Sets
No matches
HomePlasmidsYeast PlasmidspHIL-D2
PflFI - Tth111I (8055) BstZ17I (8030) NdeI (7978) BspQI - SapI (7916) AflIII - PciI (7799) PsiI (6685) SwaI (6588) PstI (6232) PvuI (6105) ScaI (5993) AatII (5555) ZraI (5553) SphI (4493) SgrAI (4337) BfuAI - BspMI (4193) AleI (88) Eco53kI (218) SacI (220) PmeI (423) BlpI (598) NsiI (688) XcmI (718) EcoRI (956) AgeI (1010) FspAI (1536) XbaI (1742) Bsu36I (1798) HpaI (1848) SalI (2887) StuI (2972) NcoI (3342) BspEI (3554) BsrGI (4008) pHIL-D2 8210 bp
PflFI  (8055)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (8055)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstZ17I  (8030)
1 site		 G T A T A C C A T A T G
NdeI  (7978)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BspQI  (7916)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (7916)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (7799)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (7799)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PsiI  (6685)
1 site		 T T A T A A A A T A T T
SwaI  (6588)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
PstI  (6232)
1 site		 C T G C A G G A C G T C
PvuI  (6105)
1 site		 C G A T C G G C T A G C
ScaI  (5993)
1 site		 A G T A C T T C A T G A
AatII  (5555)
1 site		 G A C G T C C T G C A G
ZraI  (5553)
1 site		 G A C G T C C T G C A G
SphI  (4493)
1 site		 G C A T G C C G T A C G
SgrAI  (4337)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BfuAI  (4193)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4193)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AleI  (88)
1 site		 C A C N N N N G T G G T G N N N N C A C
Eco53kI  (218)
1 site		 G A G C T C C T C G A G
SacI  (220)
1 site		 G A G C T C C T C G A G
PmeI  (423)
1 site		 G T T T A A A C C A A A T T T G
BlpI  (598)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
NsiI  (688)
1 site		 A T G C A T T A C G T A
XcmI  (718)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoRI  (956)
1 site		 G A A T T C C T T A A G
AgeI  (1010)
1 site		 A C C G G T T G G C C A
FspAI  (1536)
1 site		 R T G C G C A Y Y A C G C G T R
XbaI  (1742)
1 site		 T C T A G A A G A T C T
Bsu36I  (1798)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
HpaI  (1848)
1 site		 G T T A A C C A A T T G
SalI  (2887)
1 site		 G T C G A C C A G C T G
StuI  (2972)
1 site		 A G G C C T T C C G G A
NcoI  (3342)
1 site		 C C A T G G G G T A C C
BspEI  (3554)
1 site		 T C C G G A A G G C C T
BsrGI  (4008)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
PpHIS4
1690 .. 4224  =  2535 bp
844 amino acids  =  92.2 kDa
Product: multifunctional enzyme, required for histidine biosynthesis
auxotrophic marker for Pichia pastoris
PpHIS4
1690 .. 4224  =  2535 bp
844 amino acids  =  92.2 kDa
Product: multifunctional enzyme, required for histidine biosynthesis
auxotrophic marker for Pichia pastoris
AOX1 promoter
14 .. 953  =  940 bp
inducible promoter, regulated by methanol
AOX1 promoter
14 .. 953  =  940 bp
inducible promoter, regulated by methanol
AmpR
5687 .. 6547  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   5687 .. 5755  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5687 .. 6547  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   5756 .. 6547  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5687 .. 6547  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AOX1 3' fragment
4579 .. 5335  =  757 bp
region downstream of Pichia pastoris AOX1 gene
AOX1 3' fragment
4579 .. 5335  =  757 bp
region downstream of Pichia pastoris AOX1 gene
ori
7155 .. 7743  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7155 .. 7743  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
6589 .. 7044  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
6589 .. 7044  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AOX1 terminator
1031 .. 1277  =  247 bp
transcription terminator for AOX1
AOX1 terminator
1031 .. 1277  =  247 bp
transcription terminator for AOX1
AmpR promoter
5582 .. 5686  =  105 bp
AmpR promoter
5582 .. 5686  =  105 bp
ORF:  1360 .. 1665  =  306 bp
ORF:  101 amino acids  =  10.8 kDa
ORF:  4418 .. 4855  =  438 bp
ORF:  145 amino acids  =  15.4 kDa
ORF:  5687 .. 6547  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  477 .. 752  =  276 bp
ORF:  91 amino acids  =  10.6 kDa
ORF:  4178 .. 4492  =  315 bp
ORF:  104 amino acids  =  11.0 kDa
ORF:  1690 .. 4224  =  2535 bp
ORF:  844 amino acids  =  92.2 kDa
ORF:  4366 .. 5088  =  723 bp
ORF:  240 amino acids  =  26.3 kDa
ORF:  6151 .. 6417  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
Click here to try SnapGene

Download pHIL-D2.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

bleMX6
pFA6a-kanMX6
pPIC6(alpha) B
pYES2.1-E
hphMX6
pFA6a-kanMX6-PGAL1
pPIC6(alpha) C
pYES2 CT
kanMX
pFA6a-kanMX6-PGAL1-3HA
pPIC9
pYES2 NT A
kanMX2
pFA6a-kanMX6-PGAL1-GFP
pPIC9K
pYES2 NT B
kanMX4
pFA6a-kanMX6-PGAL1-GST
pPICZ A
pYES2 NT C
kanMX6
pFA6a-natMX6
pPICZ B
pYES3 CT
natMX6
pFA6a-TRP1
pPICZ C
pYES6 CT
pAbAi
pFA6a-TRP1-PGAL1
pPICZ(alpha) A
pYM25
pAD-GAL4
pFA6a-TRP1-PGAL1-3HA
pPICZ(alpha) B
pYM26
pAG25
pFA6a-TRP1-PGAL1-GFP
pPICZ(alpha) C
pYM44
pAG29
pFA6a-TRP1-PGAL1-GST
pPink-HC
YCp50
pAG32
pFLD
pPink-LC
YCp50-poly
pAO815
pFLD(alpha)
pPINK(alpha)-HC
YCplac111
patMX4
pGAD-C1
pRS303
YCplac22
pAUR101
pGAD-C2
pRS304
YCplac33
pAUR112
pGAD-C3
pRS305
YEp13
pAUR123
pGADT7 AD
pRS306
YEp24
pAUR224
pGADT7-Rec
pRS313
YEp351
pBridge
pGADT7-Rec (linearized)
pRS314
YEp352
pDH25
pGAPZ A
pRS315
YEp353
pEG202
pGAPZ B
pRS316
YEp354
pESC-HIS
pGAPZ C
pRS403
YEp355
pESC-LEU
pGAPZ(alpha) A
pRS404
YEp356
pESC-TRP
pGAPZ(alpha) B
pRS405
YEp356R
pESC-URA
pGAPZ(alpha) C
pRS406
YEp357
pFA6-kanMX4
pGBD-C1
pRS413
YEp357R
pFA6a
pGBD-C2
pRS414
YEp358
pFA6a-12Pk-kanMX6
pGBD-C3
pRS415
YEp358R
pFA6a-13Myc-His3MX6
pGBDU-C1
pRS416
YEp363
pFA6a-13Myc-kanMX6
pGBDU-C2
pRS423
YEp364
pFA6a-13Myc-TRP1
pGBDU-C3
pRS424
YEp365
pFA6a-3HA-His3MX6
pGBKT7
pRS425
YEp366
pFA6a-3HA-kanMX6
pGilda
pRS426
YEp366R
pFA6a-3HA-TRP1
pHIL-D2
pSpot5
YEp367
pFA6a-5FLAG-kanMX6
pHIL-S1
pSpot6
YEp367R
pFA6a-bleMX6
pJG4-5
pSpot7
YEp368
pFA6a-GFP(S65T)-His3MX6
pKLAC1
pSpot8
YEp368R
pFA6a-GFP(S65T)-kanMX6
pKLAC2
pTEF1 Zeo
YEplac112
pFA6a-GFP(S65T)-TRP1
pOAD
pUCATPH
YEplac181
pFA6a-GST-His3MX6
pOBD2
pY-secSUMOstar
YEplac195
pFA6a-GST-kanMX6
pP-secSUMO3
pY-SUMOstar
YIp351
pFA6a-GST-TRP1
pPIC3
pYC2-E
YIp352
pFA6a-His3MX6
pPIC3.5
pYC2 CT
YIp5
pFA6a-His3MX6-PGAL1
pPIC3.5K
pYC2 NT A
YIplac128
pFA6a-His3MX6-PGAL1-3HA
pPIC6 A
pYC2 NT B
YIplac204
pFA6a-His3MX6-PGAL1-GFP
pPIC6 B
pYC2 NT C
YIplac211
pFA6a-His3MX6-PGAL1-GST
pPIC6 C
pYC6 CT
YRp7
pFA6a-hphMX6
pPIC6(alpha) A
pYES2
alpha-factor secretion signal

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free