hphMX6

hphMX6 selector module conferring hygromycin resistance, for gene disruption in yeast.
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Yeast Plasmids | More Plasmid Sets
No matches
1500 1000 500 End (1576) BsmI (1563) BtgZI (1020) RsrII (756) AsiSI (712) EcoRI (591) PshAI (375) MluI (203) BseRI (150) Bpu10I (48) BmgBI (39) Start (0) hphMX6 TEF promoter HygR TEF terminator hphMX6 1576 bp
End  (1576)
0 sites
BsmI  (1563)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BtgZI  (1020)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
RsrII  (756)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AsiSI  (712)
1 site
G C G A T C G C C G C T A G C G
EcoRI  (591)
1 site
G A A T T C C T T A A G
PshAI  (375)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
MluI  (203)
1 site
A C G C G T T G C G C A
BseRI  (150)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Bpu10I  (48)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BmgBI  (39)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
Start  (0)
0 sites
hphMX6
1 .. 1576  =  1576 bp
yeast selectable marker conferring hygromycin resistance
hphMX6
1 .. 1576  =  1576 bp
yeast selectable marker conferring hygromycin resistance
TEF promoter
1 .. 344  =  344 bp
Ashbya gossypii TEF promoter
TEF promoter
1 .. 344  =  344 bp
Ashbya gossypii TEF promoter
HygR
345 .. 1373  =  1029 bp
342 amino acids  =  38.1 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
HygR
345 .. 1373  =  1029 bp
342 amino acids  =  38.1 kDa
Product: aminoglycoside phosphotransferase from E. coli
confers resistance to hygromycin
TEF terminator
1379 .. 1576  =  198 bp
Ashbya gossypii TEF terminator
TEF terminator
1379 .. 1576  =  198 bp
Ashbya gossypii TEF terminator
ORF:  345 .. 1373  =  1029 bp
ORF:  342 amino acids  =  38.1 kDa
ORF:  318 .. 1106  =  789 bp
ORF:  262 amino acids  =  29.4 kDa
Click here to try SnapGene

Download hphMX6.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.