pACEBac2

Baculovirus transfer vector with a p10 promoter and a gentamycin resistance marker. Serves as an acceptor vector in the MultiBac™ system.

Sequence Author: Geneva Biotech

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspACEBac2
BstXI (2591) AseI - PacI (2496) BbsI - BclI * (2437) SmaI (2433) TspMI - XmaI (2431) PaeR7I - XhoI (2423) NcoI (2417) BmtI (2414) NheI (2410) PvuII (2407) NsiI (2403) SphI (2396) KpnI (2390) Acc65I - BanI (2386) BsaI (2224) BanII (2146) PmeI (2090) I-CeuI (2078) Bsu36I (2075) loxP DraIII (1984) BsrGI - TatI (1811) PflFI - Tth111I (1629) BsmBI - Esp3I (1583) BtgZI (1519) BsaBI * (1417) BsrDI (1376) AgeI - BsrFI (1) BspHI (114) AlwNI (425) ApaLI (520) BaeGI - Bme1580I (524) BseYI (530) PspFI (534) BciVI (636) BssSI - BssSαI (661) BspQI - SapI (881) FspI (890) MscI (1111) EagI - SacII (1169) EcoRV (1226) BsaAI (1307) BseRI (1362) pACEBac2 2761 bp
BstXI  (2591)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
AseI  (2496)
1 site		 A T T A A T T A A T T A
PacI  (2496)
1 site		 T T A A T T A A A A T T A A T T
BbsI  (2437)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BclI  (2437)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SmaI  (2433)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (2431)
1 site		 C C C G G G G G G C C C
XmaI  (2431)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
PaeR7I  (2423)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2423)
1 site		 C T C G A G G A G C T C
NcoI  (2417)
1 site		 C C A T G G G G T A C C
BmtI  (2414)
1 site		 G C T A G C C G A T C G
NheI  (2410)
1 site		 G C T A G C C G A T C G
PvuII  (2407)
1 site		 C A G C T G G T C G A C
NsiI  (2403)
1 site		 A T G C A T T A C G T A
SphI  (2396)
1 site		 G C A T G C C G T A C G
KpnI  (2390)
1 site		 G G T A C C C C A T G G
Acc65I  (2386)
1 site		 G G T A C C C C A T G G
BanI  (2386)
1 site		 G G Y R C C C C R Y G G
Sticky ends from different BanI sites may not be compatible.
BsaI  (2224)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BanII  (2146)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
PmeI  (2090)
1 site		 G T T T A A A C C A A A T T T G
I-CeuI  (2078)
1 site		 T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T
I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences.
Bsu36I  (2075)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
DraIII  (1984)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsrGI  (1811)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (1811)
1 site		 W G T A C W W C A T G W
PflFI  (1629)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1629)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsmBI  (1583)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (1583)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
BtgZI  (1519)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaBI  (1417)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BsrDI  (1376)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
AgeI  (1)
1 site		 A C C G G T T G G C C A
BsrFI  (1)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BspHI  (114)
1 site		 T C A T G A A G T A C T
AlwNI  (425)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (520)
1 site		 G T G C A C C A C G T G
BaeGI  (524)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (524)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
BseYI  (530)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (534)
1 site		 C C C A G C G G G T C G
BciVI  (636)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSI  (661)
1 site		 C A C G A G G T G C T C
BssSαI  (661)
1 site		 C A C G A G G T G C T C
BspQI  (881)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (881)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspI  (890)
1 site		 T G C G C A A C G C G T
MscI  (1111)
1 site		 T G G C C A A C C G G T
EagI  (1169)
1 site		 C G G C C G G C C G G C
SacII  (1169)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
EcoRV  (1226)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BsaAI  (1307)
1 site		 Y A C G T R R T G C A Y
BseRI  (1362)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
ori
190 .. 778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
190 .. 778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
GmR
1203 .. 1736  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
GmR
1203 .. 1736  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
Tn7R
913 .. 1136  =  224 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7R
913 .. 1136  =  224 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7L
2596 .. 2761  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
Tn7L
2596 .. 2761  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
p10 promoter
2457 .. 2566  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
2457 .. 2566  =  110 bp
baculovirus promoter for expression in insect cells
MCS
2386 .. 2449  =  64 bp
multiple cloning site
MCS
2386 .. 2449  =  64 bp
multiple cloning site
HSV TK poly(A) signal
2273 .. 2321  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
2273 .. 2321  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal
loxP
2024 .. 2057  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
2024 .. 2057  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
Pc promoter
1925 .. 1953  =  29 bp
3 segments
   Segment 3:  -10  
   1925 .. 1930  =  6 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
3 segments
   Segment 2:  
   1931 .. 1947  =  17 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
3 segments
   Segment 1:  -35  
   1948 .. 1953  =  6 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
3 segments
class 1 integron promoter
ORF:  1838 .. 2083  =  246 bp
ORF:  81 amino acids  =  9.4 kDa
ORF:  1341 .. 1673  =  333 bp
ORF:  110 amino acids  =  12.0 kDa
ORF:  1203 .. 1736  =  534 bp
ORF:  177 amino acids  =  19.4 kDa
Click here to try SnapGene

Download pACEBac2.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free