pMT BioEase-DEST

Gateway® destination vector for inducible high-level expression of biotinylated fusion proteins in Drosophila cells.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspMT BioEase-DEST
AatII (5311) ZraI (5309) NmeAIII (4537) AhdI (4389) PspFI (3804) BseYI (3800) PciI (3496) BspQI - SapI (3380) SbfI (3129) MfeI (3020) HpaI (3011) PsiI (2991) ApaI (2837) PspOMI (2833) XbaI * (2827) BglII (2824) PacI (2821) PfoI (46) BstAPI (185) BsgI (640) AflII (852) ATG AleI (879) BstEII (881) NotI (1254) BspEI (1570) EcoRI (1574) PasI (1806) MluI (2021) BssHII (2068) NruI (2076) AgeI (2080) BstZ17I (2088) BbvCI (2278) AvaI - BsoBI - TspMI - XmaI (2422) SmaI - SrfI (2424) pMT/BioEase™-DEST 5375 bp
AatII  (5311)
1 site		 G A C G T C C T G C A G
ZraI  (5309)
1 site		 G A C G T C C T G C A G
NmeAIII  (4537)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AhdI  (4389)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspFI  (3804)
1 site		 C C C A G C G G G T C G
BseYI  (3800)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PciI  (3496)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (3380)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (3380)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SbfI  (3129)
1 site		 C C T G C A G G G G A C G T C C
MfeI  (3020)
1 site		 C A A T T G G T T A A C
HpaI  (3011)
1 site		 G T T A A C C A A T T G
PsiI  (2991)
1 site		 T T A T A A A A T A T T
ApaI  (2837)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (2833)
1 site		 G G G C C C C C C G G G
XbaI  (2827)
1 site		 T C T A G A A G A T C T
* Blocked by Dam methylation.
BglII  (2824)
1 site		 A G A T C T T C T A G A
PacI  (2821)
1 site		 T T A A T T A A A A T T A A T T
PfoI  (46)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
BstAPI  (185)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BsgI  (640)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AflII  (852)
1 site		 C T T A A G G A A T T C
AleI  (879)
1 site		 C A C N N N N G T G G T G N N N N C A C
BstEII  (881)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NotI  (1254)
1 site		 G C G G C C G C C G C C G G C G
BspEI  (1570)
1 site		 T C C G G A A G G C C T
EcoRI  (1574)
1 site		 G A A T T C C T T A A G
PasI  (1806)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
MluI  (2021)
1 site		 A C G C G T T G C G C A
BssHII  (2068)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (2076)
1 site		 T C G C G A A G C G C T
AgeI  (2080)
1 site		 A C C G G T T G G C C A
BstZ17I  (2088)
1 site		 G T A T A C C A T A T G
BbvCI  (2278)
1 site		 C C T C A G C G G A G T C G
AvaI  (2422)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2422)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (2422)
1 site		 C C C G G G G G G C C C
XmaI  (2422)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2424)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
SrfI  (2424)
1 site		 G C C C G G G C C G G G C C C G
AmpR
4316 .. 5176  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4316 .. 5107  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4316 .. 5176  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5108 .. 5176  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4316 .. 5176  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
CmR
1361 .. 2020  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1361 .. 2020  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
3557 .. 4145  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3557 .. 4145  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
MT promoter
407 .. 833  =  427 bp
Drosophila metallothionein promoter
MT promoter
407 .. 833  =  427 bp
Drosophila metallothionein promoter
ccdB
2341 .. 2646  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
2341 .. 2646  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ATG
864 .. 866  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
864 .. 866  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
BioEase™ tag
867 .. 1082  =  216 bp
72 amino acids  =  7.1 kDa
3 segments
   Segment 1:  
   867 .. 977  =  111 bp
   37 amino acids  =  3.6 kDa
Product: biotinylated sequence derived from the C-terminus of the α-subunit of Klebsiella pneumoniae oxaloacetate decarboxylase
contains a unique biotinylation site
BioEase™ tag
867 .. 1082  =  216 bp
72 amino acids  =  7.1 kDa
3 segments
   Segment 2:  site  
   978 .. 980  =  3 bp
   1 amino acid  =  146.2 Da
Product: biotinylated sequence derived from the C-terminus of the α-subunit of Klebsiella pneumoniae oxaloacetate decarboxylase
contains a unique biotinylation site
BioEase™ tag
867 .. 1082  =  216 bp
72 amino acids  =  7.1 kDa
3 segments
   Segment 3:  
   981 .. 1082  =  102 bp
   34 amino acids  =  3.4 kDa
Product: biotinylated sequence derived from the C-terminus of the α-subunit of Klebsiella pneumoniae oxaloacetate decarboxylase
contains a unique biotinylation site
BioEase™ tag
867 .. 1082  =  216 bp
72 amino acids  =  7.1 kDa
3 segments
Product: biotinylated sequence derived from the C-terminus of the α-subunit of Klebsiella pneumoniae oxaloacetate decarboxylase
contains a unique biotinylation site
Xpress™ tag
1095 .. 1118  =  24 bp
8 amino acids  =  998.0 Da
2 segments
   Segment 1:  
   1095 .. 1103  =  9 bp
   3 amino acids  =  409.4 Da
Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site
Xpress™ tag
1095 .. 1118  =  24 bp
8 amino acids  =  998.0 Da
2 segments
   Segment 2:  enterokinase site  
   1104 .. 1118  =  15 bp
   5 amino acids  =  606.5 Da
Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site
Xpress™ tag
1095 .. 1118  =  24 bp
8 amino acids  =  998.0 Da
2 segments
Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site
SV40 poly(A) signal
2877 .. 3011  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2877 .. 3011  =  135 bp
SV40 polyadenylation signal
attR1
1128 .. 1252  =  125 bp
recombination site for the Gateway® LR reaction
attR1
1128 .. 1252  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2687 .. 2811  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2687 .. 2811  =  125 bp
recombination site for the Gateway® LR reaction
AmpR promoter
5177 .. 5281  =  105 bp
AmpR promoter
5177 .. 5281  =  105 bp
lac UV5 promoter
1277 .. 1307  =  31 bp
3 segments
   Segment 1:  -35  
   1277 .. 1282  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1277 .. 1307  =  31 bp
3 segments
   Segment 2:  
   1283 .. 1300  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1277 .. 1307  =  31 bp
3 segments
   Segment 3:  -10  
   1301 .. 1307  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1277 .. 1307  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
ORF:  2341 .. 2646  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  563 .. 811  =  249 bp
ORF:  82 amino acids  =  9.2 kDa
ORF:  1361 .. 2020  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  186 .. 446  =  261 bp
ORF:  86 amino acids  =  9.9 kDa
ORF:  864 .. 1163  =  300 bp
ORF:  99 amino acids  =  10.2 kDa
ORF:  4446 .. 4712  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  405 .. 782  =  378 bp
ORF:  125 amino acids  =  13.3 kDa
ORF:  840 .. 1124  =  285 bp
ORF:  94 amino acids  =  10.3 kDa
ORF:  4316 .. 5176  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  466 .. 816  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
Click here to try SnapGene

Download pMT BioEase-DEST.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free