pIDC

Baculovirus transfer vector with a polyhedrin promoter and a chloramphenicol resistance marker. Serves as a donor vector in the MultiBac™ system.

Sequence Author: Geneva Biotech

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspIDC
BspDI - ClaI (2311) NruI (2306) BamHI (2149) RsrII (2143) BssHII (2134) StuI (2121) AccI (2112) SalI - SgrDI (2111) BanII - BsiHKAI - SacI (2109) Eco53kI (2107) XbaI (2077) PstI (2073) SfcI (2069) MfeI (1941) HpaI (1932) SmlI (1783) BpuEI (1768) BlpI (1719) AvrII (1696) PI-SceI (1671) BglII (1655) MslI (1593) MmeI (1583) BsrDI (1292) AclI (1220) BpmI - Eco57MI (1189) BstXI (22) Acc65I (64) KpnI (68) SacII (71) SnaBI (119) BstAPI (513) AflIII (531) BseYI (582) PspFI (586) Bsu36I (651) AgeI - BsrFI (700) PflFI - Tth111I (760) BaeGI - Bme1580I (842) NcoI (1004) MscI (1042) PasI (1074) pIDC 2312 bp
BspDI  (2311)
1 site		 A T C G A T T A G C T A
ClaI  (2311)
1 site		 A T C G A T T A G C T A
NruI  (2306)
1 site		 T C G C G A A G C G C T
BamHI  (2149)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
RsrII  (2143)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BssHII  (2134)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
StuI  (2121)
1 site		 A G G C C T T C C G G A
AccI  (2112)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (2111)
1 site		 G T C G A C C A G C T G
SgrDI  (2111)
1 site		 C G T C G A C G G C A G C T G C
BanII  (2109)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (2109)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
SacI  (2109)
1 site		 G A G C T C C T C G A G
Eco53kI  (2107)
1 site		 G A G C T C C T C G A G
XbaI  (2077)
1 site		 T C T A G A A G A T C T
PstI  (2073)
1 site		 C T G C A G G A C G T C
SfcI  (2069)
1 site		 C T R Y A G G A Y R T C
Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
MfeI  (1941)
1 site		 C A A T T G G T T A A C
HpaI  (1932)
1 site		 G T T A A C C A A T T G
SmlI  (1783)
1 site		 C T Y R A G G A R Y T C
Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (1768)
1 site		 C T T G A G ( N ) 14 N N G A A C T C ( N ) 14
Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BlpI  (1719)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
AvrII  (1696)
1 site		 C C T A G G G G A T C C
PI-SceI  (1671)
1 site		 A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C
PI-SceI  is a homing endonuclease that can recognize a variety of similar recognition sequences.
BglII  (1655)
1 site		 A G A T C T T C T A G A
MslI  (1593)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
MmeI  (1583)
1 site		 T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18
Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrDI  (1292)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
AclI  (1220)
1 site		 A A C G T T T T G C A A
BpmI  (1189)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (1189)
1 site		 C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BstXI  (22)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
Acc65I  (64)
1 site		 G G T A C C C C A T G G
KpnI  (68)
1 site		 G G T A C C C C A T G G
SacII  (71)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
SnaBI  (119)
1 site		 T A C G T A A T G C A T
BstAPI  (513)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
AflIII  (531)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
BseYI  (582)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (586)
1 site		 C C C A G C G G G T C G
Bsu36I  (651)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
AgeI  (700)
1 site		 A C C G G T T G G C C A
BsrFI  (700)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
PflFI  (760)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (760)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BaeGI  (842)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (842)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
NcoI  (1004)
1 site		 C C A T G G G G T A C C
MscI  (1042)
1 site		 T G G C C A A C C G G T
PasI  (1074)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
CmR
864 .. 1523  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
864 .. 1523  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
R6K γ ori
116 .. 472  =  357 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
R6K γ ori
116 .. 472  =  357 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
SV40 poly(A) signal
1798 .. 1932  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1798 .. 1932  =  135 bp
SV40 polyadenylation signal
cat promoter
1524 .. 1626  =  103 bp
promoter of the E. coli cat gene
cat promoter
1524 .. 1626  =  103 bp
promoter of the E. coli cat gene
polyhedrin promoter
2191 .. 2282  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
2191 .. 2282  =  92 bp
promoter for the baculovirus polyhedrin gene
MCS
2069 .. 2154  =  86 bp
multiple cloning site
MCS
2069 .. 2154  =  86 bp
multiple cloning site
loxP
30 .. 63  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
30 .. 63  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
ORF:  864 .. 1523  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  1713 .. 1949  =  237 bp
ORF:  78 amino acids  =  8.5 kDa
Click here to try SnapGene

Download pIDC.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free