pVL1392

Baculovirus transfer vector containing the polyhedrin promoter, for use with the BD BaculoGold™ system. For an alternative MCS, see pVL1393.

Sequence Author: BD Biosciences

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspVL1392
NdeI (9425) ScaI (8734) AlwNI (7777) PspFI (7669) BseYI (7665) BspQI - SapI (7245) FspAI (6328) SgrDI (6175) SgrAI (5906) baculovirus recombination region (ORF1629) PasI (486) PacI (583) XcmI (746) BtgI (868) SacII (871) BstEII (923) PspOMI (1395) ApaI - BanII (1399) StyI (1501) PaeR7I - PspXI - XhoI (1901) SphI (2135) BclI * (2232) BbvCI - Bpu10I (3405) baculovirus recombination region (lef2/ORF603) NgoMIV (3770) NaeI (3772) EcoRV (4000) mutated start codon BglII (4134) PstI (4142) EagI - NotI (4144) EcoRI (4155) XbaI (4159) TspMI - XmaI (4170) SmaI (4172) BamHI (4174) KflI - PpuMI (4187) SnaBI (4818) pVL1392 9639 bp
NdeI  (9425)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
ScaI  (8734)
1 site		 A G T A C T T C A T G A
AlwNI  (7777)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PspFI  (7669)
1 site		 C C C A G C G G G T C G
BseYI  (7665)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (7245)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (7245)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6328)
1 site		 R T G C G C A Y Y A C G C G T R
SgrDI  (6175)
1 site		 C G T C G A C G G C A G C T G C
SgrAI  (5906)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
PasI  (486)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
PacI  (583)
1 site		 T T A A T T A A A A T T A A T T
XcmI  (746)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BtgI  (868)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
SacII  (871)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstEII  (923)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (1395)
1 site		 G G G C C C C C C G G G
ApaI  (1399)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BanII  (1399)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
StyI  (1501)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
PaeR7I  (1901)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1901)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (1901)
1 site		 C T C G A G G A G C T C
SphI  (2135)
1 site		 G C A T G C C G T A C G
BclI  (2232)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BbvCI  (3405)
1 site		 C C T C A G C G G A G T C G
Bpu10I  (3405)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NgoMIV  (3770)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (3772)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
EcoRV  (4000)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BglII  (4134)
1 site		 A G A T C T T C T A G A
PstI  (4142)
1 site		 C T G C A G G A C G T C
EagI  (4144)
1 site		 C G G C C G G C C G G C
NotI  (4144)
1 site		 G C G G C C G C C G C C G G C G
EcoRI  (4155)
1 site		 G A A T T C C T T A A G
XbaI  (4159)
1 site		 T C T A G A A G A T C T
TspMI  (4170)
1 site		 C C C G G G G G G C C C
XmaI  (4170)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (4172)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (4174)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KflI  (4187)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
PpuMI  (4187)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SnaBI  (4818)
1 site		 T A C G T A A T G C A T
AmpR
8181 .. 9041  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8181 .. 8972  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8181 .. 9041  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8973 .. 9041  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8181 .. 9041  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
3170 .. 3997  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3170 .. 3997  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
4654 .. 5359  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
4654 .. 5359  =  706 bp
contains part of ORF1629
ori
7422 .. 8010  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7422 .. 8010  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
9042 .. 9146  =  105 bp
AmpR promoter
9042 .. 9146  =  105 bp
polyhedrin promoter
4001 .. 4092  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
4001 .. 4092  =  92 bp
promoter for the baculovirus polyhedrin gene
MCS
4134 .. 4192  =  59 bp
multiple cloning site
MCS
4134 .. 4192  =  59 bp
multiple cloning site
mutated start codon
4093 .. 4095  =  3 bp
the ATG start codon of polyhedrin was mutated to ATT
mutated start codon
4093 .. 4095  =  3 bp
the ATG start codon of polyhedrin was mutated to ATT
ORF603
3332 .. 3937  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
3332 .. 3937  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
4771 .. 5359  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
4771 .. 5359  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
3170 .. 3294  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
3170 .. 3294  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
ORF:  76 .. 582  =  507 bp
ORF:  168 amino acids  =  19.3 kDa
ORF:  2662 .. 3294  =  633 bp
ORF:  210 amino acids  =  23.9 kDa
ORF:  8311 .. 8577  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  9427 .. 15  =  228 bp
ORF:  75 amino acids  =  8.8 kDa
ORF:  4250 .. 4741  =  492 bp
ORF:  163 amino acids  =  19.1 kDa
ORF:  6395 .. 6985  =  591 bp
ORF:  196 amino acids  =  22.9 kDa
ORF:  969 .. 1241  =  273 bp
ORF:  90 amino acids  =  9.7 kDa
ORF:  2352 .. 2681  =  330 bp
ORF:  109 amino acids  =  12.4 kDa
ORF:  6819 .. 7097  =  279 bp
ORF:  92 amino acids  =  10.5 kDa
ORF:  3376 .. 3606  =  231 bp
ORF:  76 amino acids  =  9.3 kDa
ORF:  4771 .. 6396  =  1626 bp
ORF:  541 amino acids  =  60.5 kDa
ORF:  8181 .. 9041  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  614 .. 1600  =  987 bp
ORF:  328 amino acids  =  37.8 kDa
ORF:  1868 .. 2323  =  456 bp
ORF:  151 amino acids  =  17.6 kDa
ORF:  3332 .. 3937  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  4160 .. 4453  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  4478 .. 4753  =  276 bp
ORF:  91 amino acids  =  10.5 kDa
Click here to try SnapGene

Download pVL1392.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free