pIDS

Baculovirus transfer vector with a p10 promoter and a spectinomycin resistance marker. Serves as a donor vector in the MultiBac™ system.

Sequence Author: Geneva Biotech

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HomePlasmidsInsect Cell VectorspIDS
AvrII (2223) PI-SceI (2198) ApoI (2106) BssHII (2087) MmeI (2080) NaeI (2014) NgoMIV (2012) BglI (1995) BtgZI (1921) EciI (1827) BsiEI - PvuI (1744) BfuAI - BspMI (1710) BlpI (1672) BaeGI - Bme1580I - BsiHKAI (1642) ApaLI (1638) MslI (1635) SfcI (1618) BpmI (1615) BstEII (1523) AcuI (1509) EaeI (1450) BsaHI (1417) BspHI (1363) EcoP15I (1359) AgeI (1131) BspDI - ClaI (0) SpeI (5) AccI (10) BstZ17I (11) PacI (87) BbsI (140) TspMI - XmaI (146) SmaI (148) PaeR7I - SmlI - XhoI (154) NcoI (160) NheI (167) BmtI (171) NsiI (182) NspI - SphI (189) Acc65I (191) KpnI (195) BsaI (353) BanII (439) PmeI (491) BstXI (503) loxP SnaBI (549) PsiI (758) HindIII (789) AflIII (962) BseYI (1013) PspFI (1017) Bpu10I (1068) Bsu36I (1082) pIDS 2231 bp
AvrII  (2223)
1 site		 C C T A G G G G A T C C
PI-SceI  (2198)
1 site		 A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C
PI-SceI  is a homing endonuclease that can recognize a variety of similar recognition sequences.
ApoI  (2106)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
BssHII  (2087)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
MmeI  (2080)
1 site		 T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18
Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NaeI  (2014)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (2012)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BglI  (1995)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BtgZI  (1921)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
EciI  (1827)
1 site		 G G C G G A ( N ) 9 N N C C G C C T ( N ) 9
Sticky ends from different EciI sites may not be compatible.
BsiEI  (1744)
1 site		 C G R Y C G G C Y R G C
Sticky ends from different BsiEI sites may not be compatible.
PvuI  (1744)
1 site		 C G A T C G G C T A G C
BfuAI  (1710)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1710)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (1672)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BaeGI  (1642)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (1642)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
BsiHKAI  (1642)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (1638)
1 site		 G T G C A C C A C G T G
MslI  (1635)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
SfcI  (1618)
1 site		 C T R Y A G G A Y R T C
Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BpmI  (1615)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BstEII  (1523)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AcuI  (1509)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EaeI  (1450)
1 site		 Y G G C C R R C C G G Y
BsaHI  (1417)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
BspHI  (1363)
1 site		 T C A T G A A G T A C T
EcoP15I  (1359)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
AgeI  (1131)
1 site		 A C C G G T T G G C C A
BspDI  (0)
1 site		 A T C G A T T A G C T A
ClaI  (0)
1 site		 A T C G A T T A G C T A
SpeI  (5)
1 site		 A C T A G T T G A T C A
AccI  (10)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BstZ17I  (11)
1 site		 G T A T A C C A T A T G
PacI  (87)
1 site		 T T A A T T A A A A T T A A T T
BbsI  (140)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
TspMI  (146)
1 site		 C C C G G G G G G C C C
XmaI  (146)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (148)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
PaeR7I  (154)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
SmlI  (154)
1 site		 C T Y R A G G A R Y T C
Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
XhoI  (154)
1 site		 C T C G A G G A G C T C
NcoI  (160)
1 site		 C C A T G G G G T A C C
NheI  (167)
1 site		 G C T A G C C G A T C G
BmtI  (171)
1 site		 G C T A G C C G A T C G
NsiI  (182)
1 site		 A T G C A T T A C G T A
NspI  (189)
1 site		 R C A T G Y Y G T A C R
SphI  (189)
1 site		 G C A T G C C G T A C G
Acc65I  (191)
1 site		 G G T A C C C C A T G G
KpnI  (195)
1 site		 G G T A C C C C A T G G
BsaI  (353)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BanII  (439)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
PmeI  (491)
1 site		 G T T T A A A C C A A A T T T G
BstXI  (503)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
SnaBI  (549)
1 site		 T A C G T A A T G C A T
PsiI  (758)
1 site		 T T A T A A A A T A T T
HindIII  (789)
1 site		 A A G C T T T T C G A A
AflIII  (962)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
BseYI  (1013)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (1017)
1 site		 C C C A G C G G G T C G
Bpu10I  (1068)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Bsu36I  (1082)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
SmR
1365 .. 2156  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
1365 .. 2156  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
R6K γ ori
546 .. 902  =  357 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
R6K γ ori
546 .. 902  =  357 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
p10 promoter
16 .. 125  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
16 .. 125  =  110 bp
baculovirus promoter for expression in insect cells
MCS
133 .. 196  =  64 bp
multiple cloning site
MCS
133 .. 196  =  64 bp
multiple cloning site
HSV TK poly(A) signal
261 .. 309  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
261 .. 309  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal
loxP
511 .. 544  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
511 .. 544  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
ORF:  1146 .. 2156  =  1011 bp
ORF:  336 amino acids  =  37.5 kDa
ORF:  1404 .. 1787  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  1397 .. 1654  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  1664 .. 1942  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
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Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

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