pCoHygro

Selection plasmid for insect cells, with a hygromycin resistance gene under control of the copia promoter.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspCoHygro
PfoI (46) SspI (4343) XmnI (4138) BsaI (3600) AhdI (3539) AlwNI (3062) AflIII - PciI (2646) BspQI - SapI (2530) BanII - SacI (2289) Eco53kI (2287) KpnI (2283) Acc65I (2279) KasI (235) NarI (236) SfoI (237) PluTI (239) HindIII (399) SphI (409) SbfI (415) SalI (417) AccI (418) XbaI (423) PshAI (832) AsiSI (1169) RsrII (1213) SacII (1585) BbvCI (1870) BlpI (1956) PasI (2027) AleI (2056) AbsI - PaeR7I - PspXI - XhoI (2124) BsgI (2133) NheI (2134) BmtI (2138) BsmI (2223) pCoHygro 4526 bp
PfoI  (46)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
SspI  (4343)
1 site		 A A T A T T T T A T A A
XmnI  (4138)
1 site		 G A A N N N N T T C C T T N N N N A A G
BsaI  (3600)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3539)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3062)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2646)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (2646)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (2530)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (2530)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BanII  (2289)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (2289)
1 site		 G A G C T C C T C G A G
Eco53kI  (2287)
1 site		 G A G C T C C T C G A G
KpnI  (2283)
1 site		 G G T A C C C C A T G G
Acc65I  (2279)
1 site		 G G T A C C C C A T G G
KasI  (235)
1 site		 G G C G C C C C G C G G
NarI  (236)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (237)
1 site		 G G C G C C C C G C G G
PluTI  (239)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
HindIII  (399)
1 site		 A A G C T T T T C G A A
SphI  (409)
1 site		 G C A T G C C G T A C G
SbfI  (415)
1 site		 C C T G C A G G G G A C G T C C
SalI  (417)
1 site		 G T C G A C C A G C T G
AccI  (418)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
XbaI  (423)
1 site		 T C T A G A A G A T C T
PshAI  (832)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AsiSI  (1169)
1 site		 G C G A T C G C C G C T A G C G
RsrII  (1213)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
SacII  (1585)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BbvCI  (1870)
1 site		 C C T C A G C G G A G T C G
BlpI  (1956)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
PasI  (2027)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
AleI  (2056)
1 site		 C A C N N N N G T G G T G N N N N C A C
AbsI  (2124)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (2124)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2124)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (2124)
1 site		 C T C G A G G A G C T C
BsgI  (2133)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NheI  (2134)
1 site		 G C T A G C C G A T C G
BmtI  (2138)
1 site		 G C T A G C C G A T C G
BsmI  (2223)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
HygR
805 .. 1830  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
805 .. 1830  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
AmpR
3466 .. 4326  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3466 .. 4257  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3466 .. 4326  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4258 .. 4326  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3466 .. 4326  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2707 .. 3295  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2707 .. 3295  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
copia promoter
501 .. 780  =  280 bp
strong promoter from the Drosophila transposable element copia (Sinclair et al., 1986)
copia promoter
501 .. 780  =  280 bp
strong promoter from the Drosophila transposable element copia (Sinclair et al., 1986)
SV40 poly(A) signal
2143 .. 2277  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2143 .. 2277  =  135 bp
SV40 polyadenylation signal
AmpR promoter
4327 .. 4431  =  105 bp
AmpR promoter
4327 .. 4431  =  105 bp
ORF:  451 .. 684  =  234 bp
ORF:  77 amino acids  =  9.0 kDa
ORF:  781 .. 1830  =  1050 bp
ORF:  349 amino acids  =  38.8 kDa
ORF:  3596 .. 3862  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  186 .. 479  =  294 bp
ORF:  97 amino acids  =  11.2 kDa
ORF:  1836 .. 2138  =  303 bp
ORF:  100 amino acids  =  10.5 kDa
ORF:  769 .. 1563  =  795 bp
ORF:  264 amino acids  =  29.5 kDa
ORF:  3466 .. 4326  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pCoHygro.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free