pAcSG2

Compact baculovirus transfer vector for expressing proteins using the polyhedrin promoter, for use with the BD BaculoGold™ system.

Sequence Author: BD Biosciences

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HomePlasmidsInsect Cell VectorspAcSG2
PfoI (5404) EcoO109I (5347) ScaI (4851) PvuI (4741) NmeAIII (4519) BglI (4491) BpmI (4441) BsaI (4432) BmrI (4411) AlwNI (3894) PspFI (3786) BseYI (3782) BspQI - SapI (3362) HpaI (3100) BspEI (3042) BstBI (2948) baculovirus recombination region (ORF603) NgoMIV (361) NaeI (363) EcoRV (591) PaeR7I - XhoI (690) EcoRI (696) StuI (704) BtgI - NcoI - StyI (708) Eco53kI (716) BanII - SacI (718) EagI - NotI (721) PstI - SbfI (732) Acc65I (734) KpnI (738) TspMI - XmaI (740) SmaI (742) BglII (746) BsaAI - SnaBI (935) SwaI (1025) BmgBI (1439) BtgZI (1568) AgeI (1783) BspDI - ClaI (1907) SgrAI (2023) SalI - SgrDI (2292) AccI (2293) FspAI (2445) MscI (2515) pAcSG2 5544 bp
PfoI  (5404)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
EcoO109I  (5347)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
ScaI  (4851)
1 site		 A G T A C T T C A T G A
PvuI  (4741)
1 site		 C G A T C G G C T A G C
NmeAIII  (4519)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (4491)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BpmI  (4441)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (4432)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (4411)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AlwNI  (3894)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PspFI  (3786)
1 site		 C C C A G C G G G T C G
BseYI  (3782)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (3362)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (3362)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
HpaI  (3100)
1 site		 G T T A A C C A A T T G
BspEI  (3042)
1 site		 T C C G G A A G G C C T
BstBI  (2948)
1 site		 T T C G A A A A G C T T
NgoMIV  (361)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (363)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
EcoRV  (591)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PaeR7I  (690)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (690)
1 site		 C T C G A G G A G C T C
EcoRI  (696)
1 site		 G A A T T C C T T A A G
StuI  (704)
1 site		 A G G C C T T C C G G A
BtgI  (708)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NcoI  (708)
1 site		 C C A T G G G G T A C C
StyI  (708)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
Eco53kI  (716)
1 site		 G A G C T C C T C G A G
BanII  (718)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (718)
1 site		 G A G C T C C T C G A G
EagI  (721)
1 site		 C G G C C G G C C G G C
NotI  (721)
1 site		 G C G G C C G C C G C C G G C G
PstI  (732)
1 site		 C T G C A G G A C G T C
SbfI  (732)
1 site		 C C T G C A G G G G A C G T C C
Acc65I  (734)
1 site		 G G T A C C C C A T G G
KpnI  (738)
1 site		 G G T A C C C C A T G G
TspMI  (740)
1 site		 C C C G G G G G G C C C
XmaI  (740)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (742)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BglII  (746)
1 site		 A G A T C T T C T A G A
BsaAI  (935)
1 site		 Y A C G T R R T G C A Y
SnaBI  (935)
1 site		 T A C G T A A T G C A T
SwaI  (1025)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
BmgBI  (1439)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BtgZI  (1568)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AgeI  (1783)
1 site		 A C C G G T T G G C C A
BspDI  (1907)
1 site		 A T C G A T T A G C T A
ClaI  (1907)
1 site		 A T C G A T T A G C T A
SgrAI  (2023)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SalI  (2292)
1 site		 G T C G A C C A G C T G
SgrDI  (2292)
1 site		 C G T C G A C G G C A G C T G C
AccI  (2293)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
FspAI  (2445)
1 site		 R T G C G C A Y Y A C G C G T R
MscI  (2515)
1 site		 T G G C C A A C C G G T
AmpR
4298 .. 5158  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4298 .. 5089  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4298 .. 5158  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5090 .. 5158  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4298 .. 5158  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (ORF1629)
771 .. 1476  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
771 .. 1476  =  706 bp
contains part of ORF1629
ori
3539 .. 4127  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3539 .. 4127  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
baculovirus recombination region (ORF603)
1 .. 588  =  588 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF603)
1 .. 588  =  588 bp
contains ORF603 and part of lef2
AmpR promoter
5159 .. 5263  =  105 bp
AmpR promoter
5159 .. 5263  =  105 bp
polyhedrin promoter
592 .. 683  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
592 .. 683  =  92 bp
promoter for the baculovirus polyhedrin gene
MCS
690 .. 751  =  62 bp
multiple cloning site
MCS
690 .. 751  =  62 bp
multiple cloning site
ORF1629
888 .. 1476  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
888 .. 1476  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF603
1 .. 528  =  528 bp
176 amino acids  =  20.8 kDa
Product: baculovirus ORF603 protein
ORF603
1 .. 528  =  528 bp
176 amino acids  =  20.8 kDa
Product: baculovirus ORF603 protein
ATG
710 .. 712  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
710 .. 712  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ORF:  2512 .. 3102  =  591 bp
ORF:  196 amino acids  =  22.9 kDa
ORF:  2936 .. 3214  =  279 bp
ORF:  92 amino acids  =  10.5 kDa
ORF:  4428 .. 4694  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5500 .. 528  =  573 bp
ORF:  190 amino acids  =  22.2 kDa
ORF:  5514 .. 197  =  228 bp
ORF:  75 amino acids  =  9.2 kDa
ORF:  888 .. 2513  =  1626 bp
ORF:  541 amino acids  =  60.5 kDa
ORF:  4298 .. 5158  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

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