pAc5.1 V5-His C

Vector for constitutive expression of C-terminally V5-6xHis-tagged proteins in insect cells. For other reading frames, use pAc5.1/V5-His A or pAc5.1/V5-His B.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspAc5.1 V5-His C
ScaI (4865) NmeAIII (4533) AhdI (4385) DrdI (3600) BfuAI - BspMI (3442) PshAI (3104) SalI (3039) HpaI (2931) BamHI (2796) BanII - SacI (2775) Eco53kI (2773) StuI (2767) PmeI (2731) AgeI (2701) MluI (2695) BglII (132) PflMI * (383) AsiSI (391) TspMI - XmaI (425) SmaI (427) BbvCI (502) BsiWI (1096) SphI (1105) KasI (1388) NarI (1389) SfoI (1390) PluTI (1392) NsiI (1406) BspDI - ClaI (1708) AflII (1934) NdeI (2120) PpuMI (2257) BtgI - NcoI - StyI (2261) BlpI (2439) Acc65I (2579) KpnI (2583) EcoRI (2603) EcoRV (2615) NotI (2630) PaeR7I - PspXI - XhoI (2636) BstEII (2641) BstBI (2651) pAc5.1/V5-His C 5371 bp
ScaI  (4865)
1 site		 A G T A C T T C A T G A
NmeAIII  (4533)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AhdI  (4385)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DrdI  (3600)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BfuAI  (3442)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3442)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PshAI  (3104)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SalI  (3039)
1 site		 G T C G A C C A G C T G
HpaI  (2931)
1 site		 G T T A A C C A A T T G
BamHI  (2796)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BanII  (2775)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (2775)
1 site		 G A G C T C C T C G A G
Eco53kI  (2773)
1 site		 G A G C T C C T C G A G
StuI  (2767)
1 site		 A G G C C T T C C G G A
PmeI  (2731)
1 site		 G T T T A A A C C A A A T T T G
AgeI  (2701)
1 site		 A C C G G T T G G C C A
MluI  (2695)
1 site		 A C G C G T T G C G C A
BglII  (132)
1 site		 A G A T C T T C T A G A
PflMI  (383)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
AsiSI  (391)
1 site		 G C G A T C G C C G C T A G C G
TspMI  (425)
1 site		 C C C G G G G G G C C C
XmaI  (425)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (427)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BbvCI  (502)
1 site		 C C T C A G C G G A G T C G
BsiWI  (1096)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
SphI  (1105)
1 site		 G C A T G C C G T A C G
KasI  (1388)
1 site		 G G C G C C C C G C G G
NarI  (1389)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1390)
1 site		 G G C G C C C C G C G G
PluTI  (1392)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
NsiI  (1406)
1 site		 A T G C A T T A C G T A
BspDI  (1708)
1 site		 A T C G A T T A G C T A
ClaI  (1708)
1 site		 A T C G A T T A G C T A
AflII  (1934)
1 site		 C T T A A G G A A T T C
NdeI  (2120)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PpuMI  (2257)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
BtgI  (2261)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NcoI  (2261)
1 site		 C C A T G G G G T A C C
StyI  (2261)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BlpI  (2439)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
Acc65I  (2579)
1 site		 G G T A C C C C A T G G
KpnI  (2583)
1 site		 G G T A C C C C A T G G
EcoRI  (2603)
1 site		 G A A T T C C T T A A G
EcoRV  (2615)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NotI  (2630)
1 site		 G C G G C C G C C G C C G G C G
PaeR7I  (2636)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2636)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (2636)
1 site		 C T C G A G G A G C T C
BstEII  (2641)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstBI  (2651)
1 site		 T T C G A A A A G C T T
Ac5 promoter
56 .. 2568  =  2513 bp
Drosophila melanogaster actin 5C promoter
Ac5 promoter
56 .. 2568  =  2513 bp
Drosophila melanogaster actin 5C promoter
AmpR
4312 .. 5172  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   4312 .. 5103  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4312 .. 5172  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   5104 .. 5172  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4312 .. 5172  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
3553 .. 4141  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3553 .. 4141  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 poly(A) signal
2797 .. 2931  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2797 .. 2931  =  135 bp
SV40 polyadenylation signal
AmpR promoter
5173 .. 5277  =  105 bp
AmpR promoter
5173 .. 5277  =  105 bp
V5 tag
2656 .. 2697  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
2656 .. 2697  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
2707 .. 2724  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
2707 .. 2724  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
ORF:  61 .. 306  =  246 bp
ORF:  81 amino acids  =  9.7 kDa
ORF:  3022 .. 3462  =  441 bp
ORF:  146 amino acids  =  16.6 kDa
ORF:  3032 .. 3634  =  603 bp
ORF:  200 amino acids  =  22.1 kDa
ORF:  4442 .. 4708  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  726 .. 1454  =  729 bp
ORF:  242 amino acids  =  26.9 kDa
ORF:  2217 .. 2495  =  279 bp
ORF:  92 amino acids  =  10.2 kDa
ORF:  3459 .. 3878  =  420 bp
ORF:  139 amino acids  =  15.6 kDa
ORF:  1940 .. 2416  =  477 bp
ORF:  158 amino acids  =  17.7 kDa
ORF:  2954 .. 3583  =  630 bp
ORF:  209 amino acids  =  23.2 kDa
ORF:  2896 .. 3177  =  282 bp
ORF:  93 amino acids  =  10.1 kDa
ORF:  4312 .. 5172  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
Click here to try SnapGene

Download pAc5.1 V5-His C.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free