pTriEx-6 3C_LIC (linearized)

Linearized insect, bacterial, and mammalian vector for ligation-independent cloning (LIC), encoding an N-terminal Strep-Tag® II-HRV 3C and a C-terminal 10xHis.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidsInsect Cell VectorspTriEx-6 3C_LIC (linearized)
Bsu36I (71) NcoI (4965) BstBI (4992) End (5020) Start (0) DraIII (61) pTriEx™-6 3C/LIC 5020 bp
Bsu36I  (71)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4965)
1 site		 C C A T G G G G T A C C
BstBI  (4992)
1 site		 T T C G A A A A G C T T
End  (5020)
0 sites
Start  (0)
0 sites
DraIII  (61)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
AmpR
1931 .. 2791  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1931 .. 2722  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1931 .. 2791  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2723 .. 2791  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1931 .. 2791  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
3034 .. 3861  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3034 .. 3861  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
416 .. 1121  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
416 .. 1121  =  706 bp
contains part of ORF1629
ori
1331 .. 1918  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1331 .. 1918  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
Exon 1
4450 .. 4551  =  102 bp
Exon 1
4450 .. 4551  =  102 bp
intron
4552 .. 4715  =  164 bp
intron
4552 .. 4715  =  164 bp
Exon 2
4716 .. 5031  =  316 bp
Exon 2
4716 .. 5031  =  316 bp
CMV enhancer
3929 .. 4232  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
3929 .. 4232  =  304 bp
human cytomegalovirus immediate early enhancer
Exon 2
1 .. 237  =  237 bp
Exon 2
1 .. 237  =  237 bp
CMV promoter
4234 .. 4433  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4234 .. 4433  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
T7 terminator
356 .. 403  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
356 .. 403  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF603
3196 .. 3801  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
3196 .. 3801  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
533 .. 1121  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
533 .. 1121  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
lef2
3034 .. 3158  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
3034 .. 3158  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
235 .. 346  =  112 bp
rabbit globin terminator
235 .. 346  =  112 bp
p10 promoter
4843 .. 4952  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
4843 .. 4952  =  110 bp
baculovirus promoter for expression in insect cells
ATG
4967 .. 4969  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4967 .. 4969  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Strep-Tag II
4976 .. 4999  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
Strep-Tag II
4976 .. 4999  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
HRV 3C site
5006 .. 5029  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
HRV 3C site
5006 .. 5029  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
thrombin site
5 .. 22  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
thrombin site
5 .. 22  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
10xHis
29 .. 58  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
29 .. 58  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lac operator
4803 .. 4827  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
4803 .. 4827  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
4784 .. 4802  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
4784 .. 4802  =  19 bp
promoter for bacteriophage T7 RNA polymerase
poly(A) signal
213 .. 218  =  6 bp
poly(A) signal
213 .. 218  =  6 bp
RBS
4954 .. 4959  =  6 bp
ribosome binding site
RBS
4954 .. 4959  =  6 bp
ribosome binding site
ORF:  2061 .. 2327  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2841 .. 3158  =  318 bp
ORF:  105 amino acids  =  12.1 kDa
ORF:  3196 .. 3801  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  3240 .. 3470  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  4440 .. 4694  =  255 bp
ORF:  84 amino acids  =  9.0 kDa
ORF:  533 .. 1150  =  618 bp
ORF:  205 amino acids  =  23.4 kDa
ORF:  1931 .. 2791  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

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Insect Cell Vectors
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Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
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Mammalian Expression Vectors
Structural Genomics Vectors

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