pIZT V5-His

Insect cell vector with a GFP-Zeocin™ resistance fusion gene, for constitutive expression of proteins with an optional C-terminal V5-6xHis tag.

Sequence Author: Thermo Fisher (Invitrogen)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Insect Cell Vectors | More Plasmid Sets
No matches
HomePlasmidsInsect Cell VectorspIZT V5-His
BsmI (3287) DraIII (3122) BglI (3074) CsiI - SexAI * (2932) BmgBI (2903) SgrAI (2883) SmaI (2859) TspMI - XmaI (2857) AatII (2815) ZraI (2813) MauBI (2805) BtgZI (2650) MfeI (2629) BsrGI (2345) NdeI (2299) BspEI * (2291) MscI (2240) BpmI (2116) BmtI (2074) NheI (2070) AseI (1994) PmlI (1983) BlpI (1697) BspHI * (4) BsiWI (321) MslI (461) KasI (478) NarI (479) SfoI (480) PluTI (482) Acc65I (567) KpnI (571) Eco53kI (575) SacI (577) SpeI (585) EcoRI (602) EcoRV (614) NotI (629) XbaI (641) EcoO109I (647) SacII (654) BsaI (696) AgeI (708) BspDI * - ClaI * (882) PciI (945) BciVI (1148) pIZT/V5-His 3336 bp
BsmI  (3287)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
DraIII  (3122)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BglI  (3074)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
CsiI  (2932)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2932)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BmgBI  (2903)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SgrAI  (2883)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SmaI  (2859)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (2857)
1 site		 C C C G G G G G G C C C
XmaI  (2857)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
AatII  (2815)
1 site		 G A C G T C C T G C A G
ZraI  (2813)
1 site		 G A C G T C C T G C A G
MauBI  (2805)
1 site		 C G C G C G C G G C G C G C G C
BtgZI  (2650)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
MfeI  (2629)
1 site		 C A A T T G G T T A A C
BsrGI  (2345)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
NdeI  (2299)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BspEI  (2291)
1 site		 T C C G G A A G G C C T
* Blocked by Dam methylation.
MscI  (2240)
1 site		 T G G C C A A C C G G T
BpmI  (2116)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BmtI  (2074)
1 site		 G C T A G C C G A T C G
NheI  (2070)
1 site		 G C T A G C C G A T C G
AseI  (1994)
1 site		 A T T A A T T A A T T A
PmlI  (1983)
1 site		 C A C G T G G T G C A C
BlpI  (1697)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BspHI  (4)
1 site		 T C A T G A A G T A C T
* Blocked by Dam methylation.
BsiWI  (321)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
MslI  (461)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
KasI  (478)
1 site		 G G C G C C C C G C G G
NarI  (479)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (480)
1 site		 G G C G C C C C G C G G
PluTI  (482)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
Acc65I  (567)
1 site		 G G T A C C C C A T G G
KpnI  (571)
1 site		 G G T A C C C C A T G G
Eco53kI  (575)
1 site		 G A G C T C C T C G A G
SacI  (577)
1 site		 G A G C T C C T C G A G
SpeI  (585)
1 site		 A C T A G T T G A T C A
EcoRI  (602)
1 site		 G A A T T C C T T A A G
EcoRV  (614)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NotI  (629)
1 site		 G C G G C C G C C G C C G G C G
XbaI  (641)
1 site		 T C T A G A A G A T C T
EcoO109I  (647)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
SacII  (654)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BsaI  (696)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AgeI  (708)
1 site		 A C C G G T T G G C C A
BspDI  (882)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (882)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
PciI  (945)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BciVI  (1148)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
ATG
2052 .. 2054  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2052 .. 2054  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Cycle 3 GFP
2067 .. 2765  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996) lacking the unstructured C-terminal peptide
mammalian codon-optimized
Cycle 3 GFP
2067 .. 2765  =  699 bp
233 amino acids  =  26.1 kDa
Product: Cycle 3 GFP (Crameri et al., 1996) lacking the unstructured C-terminal peptide
mammalian codon-optimized
BleoR
2766 .. 3143  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
2766 .. 3143  =  378 bp
125 amino acids  =  13.9 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
ori
1006 .. 1594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1006 .. 1594  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
OpIE-2 promoter
5 .. 552  =  548 bp
strong constitutive baculovirus promoter for insect cell expression
OpIE-2 promoter
5 .. 552  =  548 bp
strong constitutive baculovirus promoter for insect cell expression
OpIE-1 promoter
1669 .. 1960  =  292 bp
moderate constitutive baculovirus promoter for insect cell expression
OpIE-1 promoter
1669 .. 1960  =  292 bp
moderate constitutive baculovirus promoter for insect cell expression
SV40 poly(A) signal
3207 .. 4  =  134 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3207 .. 4  =  134 bp
SV40 polyadenylation signal
OpIE-2 poly(A) signal
749 .. 878  =  130 bp
baculovirus polyadenylation signal
OpIE-2 poly(A) signal
749 .. 878  =  130 bp
baculovirus polyadenylation signal
MCS
567 .. 656  =  90 bp
multiple cloning site
MCS
567 .. 656  =  90 bp
multiple cloning site
V5 tag
663 .. 704  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
663 .. 704  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
714 .. 731  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
714 .. 731  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
EM7 promoter
1986 .. 2033  =  48 bp
synthetic bacterial promoter
EM7 promoter
1986 .. 2033  =  48 bp
synthetic bacterial promoter
ORF:  19 .. 249  =  231 bp
ORF:  76 amino acids  =  8.9 kDa
ORF:  2052 .. 3143  =  1092 bp
ORF:  363 amino acids  =  40.5 kDa
ORF:  1651 .. 2007  =  357 bp
ORF:  118 amino acids  =  13.2 kDa
ORF:  2666 .. 3049  =  384 bp
ORF:  127 amino acids  =  13.7 kDa
Click here to try SnapGene

Download pIZT V5-His.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free