pTriEx-6

Insect, bacterial, and mammalian vector, encoding a N-terminal Strep-Tag® II-HRV 3C cassette and a cleavable C-terminal 10xHis tag.

Sequence Author: MilliporeSigma (Novagen)

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HomePlasmidsInsect Cell VectorspTriEx-6
NcoI (2112) BstBI (2139) EcoNI (2165) SanDI (2171) TspMI - XmaI (2174) SmaI (2176) Acc65I (2178) KpnI (2182) BamHI (2185) EagI - NotI (2205) Eco53kI (2215) SacI (2217) DraIII (2278) Bsu36I (2288) pTriEx™-6 5070 bp
NcoI  (2112)
1 site		 C C A T G G G G T A C C
BstBI  (2139)
1 site		 T T C G A A A A G C T T
EcoNI  (2165)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SanDI  (2171)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different SanDI sites may not be compatible.
TspMI  (2174)
1 site		 C C C G G G G G G C C C
XmaI  (2174)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2176)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
Acc65I  (2178)
1 site		 G G T A C C C C A T G G
KpnI  (2182)
1 site		 G G T A C C C C A T G G
BamHI  (2185)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EagI  (2205)
1 site		 C G G C C G G C C G G C
NotI  (2205)
1 site		 G C G G C C G C C G C C G G C G
Eco53kI  (2215)
1 site		 G A G C T C C T C G A G
SacI  (2217)
1 site		 G A G C T C C T C G A G
DraIII  (2278)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
Bsu36I  (2288)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
AmpR
4148 .. 5008  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4148 .. 4939  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4148 .. 5008  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4940 .. 5008  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4148 .. 5008  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Exon 1
1597 .. 1698  =  102 bp
Exon 1
1597 .. 1698  =  102 bp
intron
1699 .. 1862  =  164 bp
intron
1699 .. 1862  =  164 bp
Exon 2
1863 .. 2454  =  592 bp
Exon 2
1863 .. 2454  =  592 bp
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
2633 .. 3338  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
2633 .. 3338  =  706 bp
contains part of ORF1629
ori
3548 .. 4135  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3548 .. 4135  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
1076 .. 1379  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1076 .. 1379  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
1381 .. 1580  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
1381 .. 1580  =  200 bp
human cytomegalovirus (CMV) immediate early promoter
T7 terminator
2573 .. 2620  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
2573 .. 2620  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
2750 .. 3338  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
2750 .. 3338  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ATG
2114 .. 2116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2114 .. 2116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Strep-Tag II
2123 .. 2146  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
Strep-Tag II
2123 .. 2146  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin®, an engineered form of streptavidin
HRV 3C site
2153 .. 2176  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
HRV 3C site
2153 .. 2176  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
thrombin site
2222 .. 2239  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
thrombin site
2222 .. 2239  =  18 bp
6 amino acids  =  627.7 Da
Product: thrombin recognition and cleavage site
10xHis
2246 .. 2275  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
10xHis
2246 .. 2275  =  30 bp
10 amino acids  =  1.4 kDa
Product: 10xHis affinity tag
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
2452 .. 2563  =  112 bp
rabbit globin terminator
2452 .. 2563  =  112 bp
p10 promoter
1990 .. 2099  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
1990 .. 2099  =  110 bp
baculovirus promoter for expression in insect cells
lac operator
1950 .. 1974  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1950 .. 1974  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
1931 .. 1949  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1931 .. 1949  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
2101 .. 2106  =  6 bp
ribosome binding site
RBS
2101 .. 2106  =  6 bp
ribosome binding site
poly(A) signal
2430 .. 2435  =  6 bp
poly(A) signal
2430 .. 2435  =  6 bp
MCS
2174 .. 2218  =  45 bp
multiple cloning site
MCS
2174 .. 2218  =  45 bp
multiple cloning site
ORF:  4278 .. 4544  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  5058 .. 305  =  318 bp
ORF:  105 amino acids  =  12.1 kDa
ORF:  343 .. 948  =  606 bp
ORF:  201 amino acids  =  23.6 kDa
ORF:  387 .. 617  =  231 bp
ORF:  76 amino acids  =  9.2 kDa
ORF:  1587 .. 1841  =  255 bp
ORF:  84 amino acids  =  9.0 kDa
ORF:  2081 .. 2416  =  336 bp
ORF:  111 amino acids  =  12.3 kDa
ORF:  2750 .. 3367  =  618 bp
ORF:  205 amino acids  =  23.4 kDa
ORF:  4148 .. 5008  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

BaculoDirect C-Term Linear DNA
pBACgus-5
pIEx Bac-1
pMT BiP V5-His A
BaculoDirect N-Term Linear DNA
pBACgus-6
pIEx Bac-1 Ek_LIC (linearized)
pMT BiP V5-His B
pAB-6xHis-MBP
pBACgus4x-1
pIEx Bac-3
pMT BiP V5-His C
pAB-6xHis
pBacPAK8
pIEx Bac-3 3C_LIC (linearized)
pMT V5-His A
pAB-bee
pBacPAK9
pIEx Bac-4
pMT V5-His B
pAB-bee-8xHis
pBACsurf-1
pIEx Bac-4 Ek_LIC (linearized)
pMT V5-His C
pAB-bee-FH
pBAC-1
pIEx Bac-5
pQE-TriSystem
pAB-GST
pBAC-2cp
pIEx Bac-5 3C_LIC (linearized)
pQE-TriSystem His-Strep1
pAB-MBP
pBAC-3
pIEx-1
pQE-TriSystem His-Strep2
pAc5.1 V5-His A
pBAC-5
pIEx-10
pQE-TriSystem Strep
pAc5.1 V5-His B
pBAC-6
pIEx-10 Ek_LIC
pQE-Trisystem-5
pAc5.1 V5-His C
pBiEx-1
pIEx-10 Ek_LIC (linearized)
pQE-Trisystem-6
pAcAB3
pBiEx-2
pIEx-2
pQE-TriSystem-7
pAcAB4
pBiEx-3
pIEx-3
pTriEx-1.1
pACEBac1
pCoBlast
pIEx-4
pTriEx-1.1 Hygro
pACEBac2
pCoHygro
pIEx-5
pTriEx-1.1 Neo
pAcG2T
pDEST10
pIEx-6
pTriEx-2
pAcG3X
pDEST20
pIEx-7 Ek_LIC
pTriEx-2 Hygro
pAcGHLT A
pDEST8
pIEx-7 Ek_LIC (linearized)
pTriEx-2 Neo
pAcGHLT B
pDream2.1 MCS
pIEx-8
pTriEx-3
pAcGHLT C
pFastBac Dual
pIEx-8 Ek_LIC
pTriEx-3 Hygro
pAcGP67-A
pFastBac CT-TOPO (linearized)
pIEx-8 Ek_LIC (linearized)
pTriEx-3 Neo
pAcGP67-B
pFastBac HBM-TOPO (linearized)
pIEx-9
pTriEx-4
pAcGP67-C
pFastBac NT-TOPO (linearized)
pIEx-9 3C_LIC
pTriEx-4 Ek_LIC (linearized)
pAcHLT A
pFastBac1
pIEx-9 3C_LIC (linearized)
pTriEx-4 Hygro
pAcHLT B
pFastBacHT A
pIZ V5-His
pTriEx-4 Neo
pAcHLT C
pFastBacHT B
pIZT V5-His
pTriEx-5
pAcMP2
pFastBacHT C
pMelBac A
pTriEx-5 Ek_LIC (linearized)
pAcMP3
pI-secSUMOstar
pMelBac B
pTriEx-6
pAcSecG2T
pI-SUMOstar
pMelBac C
pTriEx-6 3C_LIC (linearized)
pAcSG2
pIB V5-His
pMIB V5-His A
pTriEx-7
pAcUW51
pIB V5-His-DEST
pMIB V5-His B
pTriEx-7 Ek_LIC (linearized)
pBAC4x-1
pIDC
pMIB V5-His C
pVL-FH
pBACgus-1
pIDK
pMT-DEST48
pVL1392
pBACgus-2cp
pIDS
pMT BioEase-DEST
pVL1393
pBACgus-3

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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