pBI121

Binary Agrobacterium vector with a GUS reporter gene for plant transformation.
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HomePlasmidsPlant VectorspBI121
NotI (14,662) PasI (14,110) BstEII (13,001) BstXI (12,671) SfiI (11,038) PflMI * (10,709) NdeI (10,475) FspAI (9695) KflI (9097) BbvCI (9007) AhdI (8953) DraIII (8290) M13 fwd EcoRI (7982) SacI (7715) EcoNI (1627) RB T-DNA repeat PmeI (2492) Bsu36I (2700) NheI (2715) BmtI (2719) PspOMI (3899) ApaI (3903) BspDI - ClaI (4537) lac operator M13 rev HindIII (4950) SbfI (4966) BsaI (5045) ScaI (5179) XbaI (5815) BamHI (5821) TspMI - XmaI (5826) SmaI (5828) SnaBI (6229) Eco53kI (7713) pBI121 14,758 bp
NotI  (14,662)
1 site		 G C G G C C G C C G C C G G C G
PasI  (14,110)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
BstEII  (13,001)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (12,671)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
SfiI  (11,038)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PflMI  (10,709)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
NdeI  (10,475)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
FspAI  (9695)
1 site		 R T G C G C A Y Y A C G C G T R
KflI  (9097)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
BbvCI  (9007)
1 site		 C C T C A G C G G A G T C G
AhdI  (8953)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (8290)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
EcoRI  (7982)
1 site		 G A A T T C C T T A A G
SacI  (7715)
1 site		 G A G C T C C T C G A G
EcoNI  (1627)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PmeI  (2492)
1 site		 G T T T A A A C C A A A T T T G
Bsu36I  (2700)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NheI  (2715)
1 site		 G C T A G C C G A T C G
BmtI  (2719)
1 site		 G C T A G C C G A T C G
PspOMI  (3899)
1 site		 G G G C C C C C C G G G
ApaI  (3903)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BspDI  (4537)
1 site		 A T C G A T T A G C T A
ClaI  (4537)
1 site		 A T C G A T T A G C T A
HindIII  (4950)
1 site		 A A G C T T T T C G A A
SbfI  (4966)
1 site		 C C T G C A G G G G A C G T C C
BsaI  (5045)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ScaI  (5179)
1 site		 A G T A C T T C A T G A
XbaI  (5815)
1 site		 T C T A G A A G A T C T
BamHI  (5821)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
TspMI  (5826)
1 site		 C C C G G G G G G C C C
XmaI  (5826)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (5828)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
SnaBI  (6229)
1 site		 T A C G T A A T G C A T
Eco53kI  (7713)
1 site		 G A G C T C C T C G A G
GUS
5845 .. 7656  =  1812 bp
603 amino acids  =  68.4 kDa
Product: β-glucuronidase
GUS
5845 .. 7656  =  1812 bp
603 amino acids  =  68.4 kDa
Product: β-glucuronidase
trfA
10,242 .. 11,390  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
trfA
10,242 .. 11,390  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
NeoR/KanR
2838 .. 3632  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
2838 .. 3632  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
KanR
11,689 .. 12,483  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
11,689 .. 12,483  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
IS1
12,675 .. 13,442  =  768 bp
prokaryotic transposable element
IS1
12,675 .. 13,442  =  768 bp
prokaryotic transposable element
oriV
14,065 .. 18  =  712 bp
incP origin of replication
oriV
14,065 .. 18  =  712 bp
incP origin of replication
TetR
1339 .. 1989  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
1339 .. 1989  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
traJ
145 .. 516  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
145 .. 516  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
CaMV 35S promoter
5461 .. 5806  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
5461 .. 5806  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
NOS terminator
4022 .. 4274  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
4022 .. 4274  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
7727 .. 7979  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
7727 .. 7979  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
2602 .. 2781  =  180 bp
nopaline synthase promoter
NOS promoter
2602 .. 2781  =  180 bp
nopaline synthase promoter
oriT
549 .. 658  =  110 bp
incP origin of transfer
oriT
549 .. 658  =  110 bp
incP origin of transfer
lac promoter
4859 .. 4889  =  31 bp
3 segments
   Segment 1:  -35  
   4859 .. 4864  =  6 bp
promoter for the E. coli lac operon
lac promoter
4859 .. 4889  =  31 bp
3 segments
   Segment 2:  
   4865 .. 4882  =  18 bp
promoter for the E. coli lac operon
lac promoter
4859 .. 4889  =  31 bp
3 segments
   Segment 3:  -10  
   4883 .. 4889  =  7 bp
promoter for the E. coli lac operon
lac promoter
4859 .. 4889  =  31 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
2454 .. 2478  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
2454 .. 2478  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
8622 .. 8646  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
8622 .. 8646  =  25 bp
left border repeat from nopaline C58 T-DNA
lac operator
4897 .. 4913  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
4897 .. 4913  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
M13 rev
4921 .. 4937  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
4921 .. 4937  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
7988 .. 8004  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
7988 .. 8004  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  2020 .. 2433  =  414 bp
ORF:  137 amino acids  =  14.9 kDa
ORF:  3010 .. 3396  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  5845 .. 7656  =  1812 bp
ORF:  603 amino acids  =  68.4 kDa
ORF:  9826 .. 10,092  =  267 bp
ORF:  88 amino acids  =  9.8 kDa
ORF:  10,861 .. 11,448  =  588 bp
ORF:  195 amino acids  =  20.7 kDa
ORF:  3653 .. 4045  =  393 bp
ORF:  130 amino acids  =  14.4 kDa
ORF:  4958 .. 5707  =  750 bp
ORF:  249 amino acids  =  28.0 kDa
ORF:  11,822 .. 12,052  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  13,775 .. 14,215  =  441 bp
ORF:  146 amino acids  =  16.0 kDa
ORF:  2838 .. 3632  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  4428 .. 4766  =  339 bp
ORF:  112 amino acids  =  11.7 kDa
ORF:  5001 .. 5231  =  231 bp
ORF:  76 amino acids  =  9.1 kDa
ORF:  10,254 .. 10,649  =  396 bp
ORF:  131 amino acids  =  14.7 kDa
ORF:  12,924 .. 13,427  =  504 bp
ORF:  167 amino acids  =  19.6 kDa
ORF:  13,830 .. 14,342  =  513 bp
ORF:  170 amino acids  =  17.9 kDa
ORF:  10,529 .. 10,801  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  11,387 .. 11,668  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  13,103 .. 13,393  =  291 bp
ORF:  96 amino acids  =  11.0 kDa
ORF:  13,766 .. 14,071  =  306 bp
ORF:  101 amino acids  =  9.6 kDa
ORF:  145 .. 516  =  372 bp
ORF:  123 amino acids  =  13.5 kDa
ORF:  1339 .. 2016  =  678 bp
ORF:  225 amino acids  =  24.3 kDa
ORF:  2035 .. 2376  =  342 bp
ORF:  113 amino acids  =  13.0 kDa
ORF:  3712 .. 4092  =  381 bp
ORF:  126 amino acids  =  13.7 kDa
ORF:  5959 .. 6420  =  462 bp
ORF:  153 amino acids  =  17.5 kDa
ORF:  7009 .. 7335  =  327 bp
ORF:  108 amino acids  =  12.8 kDa
ORF:  7387 .. 7797  =  411 bp
ORF:  136 amino acids  =  15.4 kDa
ORF:  9076 .. 9987  =  912 bp
ORF:  303 amino acids  =  32.1 kDa
ORF:  10,267 .. 10,662  =  396 bp
ORF:  131 amino acids  =  14.1 kDa
ORF:  11,689 .. 12,483  =  795 bp
ORF:  264 amino acids  =  31.0 kDa
ORF:  14,620 .. 89  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  3147 .. 3683  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  8544 .. 8768  =  225 bp
ORF:  74 amino acids  =  8.2 kDa
ORF:  10,242 .. 11,390  =  1149 bp
ORF:  382 amino acids  =  43.8 kDa
ORF:  11,439 .. 11,681  =  243 bp
ORF:  80 amino acids  =  9.0 kDa
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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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