pGreenII 0179

Agrobacterium binary vector with kanamycin- and hygromycin-resistance genes.

Sequence Author: pGreen website

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HomePlasmidsPlant VectorspGreenII 0179
NruI (4821) PasI (4603) EcoNI (4565) SspI (4553) PflMI (4218) AlwNI (3691) BciVI (3478) BglII (3270) StuI (3217) BspQI - SapI (3093) lac operator SacI (2814) Eco53kI (2812) BstXI (2806) SacII (2805) AleI - MslI (2804) NotI (2793) XbaI (2786) SpeI (2780) BamHI (2774) SmaI (2770) TspMI - XmaI (2768) PstI (2766) EcoRI (2756) EcoRV (2752) HindIII (2744) AccI (2730) SalI (2729) AbsI - PaeR7I - PspXI - XhoI (2723) ApaI (2718) EcoO109I (2715) PspOMI (2714) KpnI (2712) Acc65I (2708) BsaBI * (62) StyI (421) NgoMIV (524) NaeI (526) FseI (528) AanI - PsiI (546) BglII (627) LB T-DNA repeat BlpI (919) BbvCI (1005) BtgZI (1397) NdeI (1621) RsrII (1662) BfuAI - BspMI (1756) ZraI (2048) AatII (2050) BmgBI (2211) FspI (2537) BmrI (2638) pGreenII 0179 5138 bp
NruI  (4821)
1 site		 T C G C G A A G C G C T
PasI  (4603)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
EcoNI  (4565)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (4553)
1 site		 A A T A T T T T A T A A
PflMI  (4218)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
AlwNI  (3691)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BciVI  (3478)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BglII  (3270)
2 sites		 A G A T C T T C T A G A
StuI  (3217)
1 site		 A G G C C T T C C G G A
BspQI  (3093)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (3093)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SacI  (2814)
1 site		 G A G C T C C T C G A G
Eco53kI  (2812)
1 site		 G A G C T C C T C G A G
BstXI  (2806)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
SacII  (2805)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (2804)
1 site		 C A C N N N N G T G G T G N N N N C A C
MslI  (2804)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
NotI  (2793)
1 site		 G C G G C C G C C G C C G G C G
XbaI  (2786)
1 site		 T C T A G A A G A T C T
SpeI  (2780)
1 site		 A C T A G T T G A T C A
BamHI  (2774)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (2770)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (2768)
1 site		 C C C G G G G G G C C C
XmaI  (2768)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
PstI  (2766)
1 site		 C T G C A G G A C G T C
EcoRI  (2756)
1 site		 G A A T T C C T T A A G
EcoRV  (2752)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HindIII  (2744)
1 site		 A A G C T T T T C G A A
AccI  (2730)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (2729)
1 site		 G T C G A C C A G C T G
AbsI  (2723)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (2723)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2723)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (2723)
1 site		 C T C G A G G A G C T C
ApaI  (2718)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
EcoO109I  (2715)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (2714)
1 site		 G G G C C C C C C G G G
KpnI  (2712)
1 site		 G G T A C C C C A T G G
Acc65I  (2708)
1 site		 G G T A C C C C A T G G
BsaBI  (62)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
StyI  (421)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
NgoMIV  (524)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (526)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
FseI  (528)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
AanI  (546)
1 site		 T T A T A A A A T A T T
PsiI  (546)
1 site		 T T A T A A A A T A T T
BglII  (627)
2 sites		 A G A T C T T C T A G A
BlpI  (919)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BbvCI  (1005)
1 site		 C C T C A G C G G A G T C G
BtgZI  (1397)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
NdeI  (1621)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
RsrII  (1662)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BfuAI  (1756)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1756)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
ZraI  (2048)
1 site		 G A C G T C C T G C A G
AatII  (2050)
1 site		 G A C G T C C T G C A G
BmgBI  (2211)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
FspI  (2537)
1 site		 T G C G C A A C G C G T
BmrI  (2638)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
HygR
1049 .. 2074  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
1049 .. 2074  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
KanR
4095 .. 4910  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
4095 .. 4910  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
ori
3336 .. 3924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3336 .. 3924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
lacZα
2434 .. 2871  =  438 bp
145 amino acids  =  16.2 kDa
Product: LacZα fragment of β-galactosidase
lacZα
2434 .. 2871  =  438 bp
145 amino acids  =  16.2 kDa
Product: LacZα fragment of β-galactosidase
pSa ori
63 .. 498  =  436 bp
origin of replication from bacterial plasmid pSa
pSa ori
63 .. 498  =  436 bp
origin of replication from bacterial plasmid pSa
CaMV poly(A) signal
663 .. 839  =  177 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
663 .. 839  =  177 bp
cauliflower mosaic virus polyadenylation signal
lac promoter
2915 .. 2946  =  32 bp
3 segments
   Segment 3:  -10  
   2915 .. 2921  =  7 bp
promoter for the E. coli lac operon
lac promoter
2915 .. 2946  =  32 bp
3 segments
   Segment 2:  
   2922 .. 2940  =  19 bp
promoter for the E. coli lac operon
lac promoter
2915 .. 2946  =  32 bp
3 segments
   Segment 1:  -35  
   2941 .. 2946  =  6 bp
promoter for the E. coli lac operon
lac promoter
2915 .. 2946  =  32 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
3221 .. 3245  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
3221 .. 3245  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
633 .. 655  =  23 bp
left border repeat from nopaline C58 T-DNA (truncated)
LB T-DNA repeat
633 .. 655  =  23 bp
left border repeat from nopaline C58 T-DNA (truncated)
lac operator
2891 .. 2907  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
2891 .. 2907  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
CaMV 35S promoter
2091 .. 2436  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
2091 .. 2436  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
MCS
2708 .. 2815  =  108 bp
pBluescript multiple cloning site
MCS
2708 .. 2815  =  108 bp
pBluescript multiple cloning site
T7 promoter
2681 .. 2699  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2681 .. 2699  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T3 promoter
2828 .. 2846  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T3 promoter
2828 .. 2846  =  19 bp
promoter for bacteriophage T3 RNA polymerase
M13 fwd
2658 .. 2674  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
2658 .. 2674  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
2867 .. 2883  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
2867 .. 2883  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  1316 .. 2125  =  810 bp
ORF:  269 amino acids  =  30.3 kDa
ORF:  3257 .. 3661  =  405 bp
ORF:  134 amino acids  =  15.0 kDa
ORF:  4073 .. 4300  =  228 bp
ORF:  75 amino acids  =  8.8 kDa
ORF:  2190 .. 2498  =  309 bp
ORF:  102 amino acids  =  11.3 kDa
ORF:  4095 .. 4910  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
ORF:  1049 .. 2164  =  1116 bp
ORF:  371 amino acids  =  41.6 kDa
ORF:  2434 .. 2871  =  438 bp
ORF:  145 amino acids  =  16.2 kDa
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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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