pSoup

Plant vector used for co-transformation with pGreen family plasmids.

Sequence Author: pGreen website

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BclI * (9210) RsrII (9105) AatII (8338) ZraI (8336) PmlI (7830) AgeI (7762) MfeI (5975) AleI (5705) NdeI (5579) PfoI * (5487) SfiI (5021) PciI (235) DrdI (343) NotI - SacII (1367) MscI (1514) PsiI (1806) PasI (1920) Eco53kI (2450) SacI (2452) Acc65I (2454) KpnI - TspMI - XmaI (2458) SmaI (2460) BamHI (2463) XbaI (2469) SalI (2475) PstI - SbfI (2485) SphI (2491) HindIII (2493) SP6 promoter lac operator PvuII (2706) XmnI (2890) tet promoter EcoRV (3079) NheI (3121) BmtI (3125) PshAI (3608) pSoup 9275 bp
BclI  (9210)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
RsrII  (9105)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AatII  (8338)
1 site		 G A C G T C C T G C A G
ZraI  (8336)
1 site		 G A C G T C C T G C A G
PmlI  (7830)
1 site		 C A C G T G G T G C A C
AgeI  (7762)
1 site		 A C C G G T T G G C C A
MfeI  (5975)
1 site		 C A A T T G G T T A A C
AleI  (5705)
1 site		 C A C N N N N G T G G T G N N N N C A C
NdeI  (5579)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PfoI  (5487)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SfiI  (5021)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PciI  (235)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
DrdI  (343)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
NotI  (1367)
1 site		 G C G G C C G C C G C C G G C G
SacII  (1367)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
MscI  (1514)
1 site		 T G G C C A A C C G G T
PsiI  (1806)
1 site		 T T A T A A A A T A T T
PasI  (1920)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
Eco53kI  (2450)
1 site		 G A G C T C C T C G A G
SacI  (2452)
1 site		 G A G C T C C T C G A G
Acc65I  (2454)
1 site		 G G T A C C C C A T G G
KpnI  (2458)
1 site		 G G T A C C C C A T G G
TspMI  (2458)
1 site		 C C C G G G G G G C C C
XmaI  (2458)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2460)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (2463)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (2469)
1 site		 T C T A G A A G A T C T
SalI  (2475)
1 site		 G T C G A C C A G C T G
PstI  (2485)
1 site		 C T G C A G G A C G T C
SbfI  (2485)
1 site		 C C T G C A G G G G A C G T C C
SphI  (2491)
1 site		 G C A T G C C G T A C G
HindIII  (2493)
1 site		 A A G C T T T T C G A A
PvuII  (2706)
1 site		 C A G C T G G T C G A C
XmnI  (2890)
1 site		 G A A N N N N T T C C T T N N N N A A G
EcoRV  (3079)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NheI  (3121)
1 site		 G C T A G C C G A T C G
BmtI  (3125)
1 site		 G C T A G C C G A T C G
PshAI  (3608)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
TcR
2978 .. 4168  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
2978 .. 4168  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
trfA
4667 .. 5815  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
trfA
4667 .. 5815  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
RepA
7196 .. 8167  =  972 bp
323 amino acids  =  36.5 kDa
Product: replication protein of plasmid pSa
RepA
7196 .. 8167  =  972 bp
323 amino acids  =  36.5 kDa
Product: replication protein of plasmid pSa
oriV
1258 .. 1969  =  712 bp
incP origin of replication
oriV
1258 .. 1969  =  712 bp
incP origin of replication
TetR
8562 .. 9212  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
8562 .. 9212  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
traJ
760 .. 1131  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
760 .. 1131  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
oriT
618 .. 727  =  110 bp
incP origin of transfer
oriT
618 .. 727  =  110 bp
incP origin of transfer
MCS
2448 .. 2498  =  51 bp
multiple cloning site
MCS
2448 .. 2498  =  51 bp
multiple cloning site
lac promoter
2589 .. 2619  =  31 bp
3 segments
   Segment 3:  -10  
   2589 .. 2595  =  7 bp
promoter for the E. coli lac operon
lac promoter
2589 .. 2619  =  31 bp
3 segments
   Segment 2:  
   2596 .. 2613  =  18 bp
promoter for the E. coli lac operon
lac promoter
2589 .. 2619  =  31 bp
3 segments
   Segment 1:  -35  
   2614 .. 2619  =  6 bp
promoter for the E. coli lac operon
lac promoter
2589 .. 2619  =  31 bp
3 segments
promoter for the E. coli lac operon
tet promoter
2902 .. 2930  =  29 bp
3 segments
   Segment 1:  -35  
   2902 .. 2907  =  6 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
2902 .. 2930  =  29 bp
3 segments
   Segment 2:  
   2908 .. 2924  =  17 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
2902 .. 2930  =  29 bp
3 segments
   Segment 3:  -10  
   2925 .. 2930  =  6 bp
E. coli promoter for tetracycline efflux protein gene
tet promoter
2902 .. 2930  =  29 bp
3 segments
E. coli promoter for tetracycline efflux protein gene
SP6 promoter
2505 .. 2523  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
2505 .. 2523  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
M13 rev
2541 .. 2557  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
2541 .. 2557  =  17 bp
common sequencing primer, one of multiple similar variants
lac operator
2565 .. 2581  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
2565 .. 2581  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  760 .. 1131  =  372 bp
ORF:  123 amino acids  =  13.5 kDa
ORF:  1963 .. 2268  =  306 bp
ORF:  101 amino acids  =  9.6 kDa
ORF:  3151 .. 3534  =  384 bp
ORF:  127 amino acids  =  14.7 kDa
ORF:  4072 .. 4368  =  297 bp
ORF:  98 amino acids  =  11.4 kDa
ORF:  5395 .. 5790  =  396 bp
ORF:  131 amino acids  =  14.1 kDa
ORF:  6070 .. 6912  =  843 bp
ORF:  280 amino acids  =  29.7 kDa
ORF:  8086 .. 8325  =  240 bp
ORF:  79 amino acids  =  8.7 kDa
ORF:  1187 .. 1414  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  2978 .. 4168  =  1191 bp
ORF:  396 amino acids  =  41.5 kDa
ORF:  4376 .. 4618  =  243 bp
ORF:  80 amino acids  =  9.0 kDa
ORF:  4667 .. 5815  =  1149 bp
ORF:  382 amino acids  =  43.8 kDa
ORF:  7196 .. 8167  =  972 bp
ORF:  323 amino acids  =  36.5 kDa
ORF:  4389 .. 4670  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  5256 .. 5528  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  8076 .. 8516  =  441 bp
ORF:  146 amino acids  =  16.2 kDa
ORF:  8535 .. 9212  =  678 bp
ORF:  225 amino acids  =  24.3 kDa
ORF:  1692 .. 2204  =  513 bp
ORF:  170 amino acids  =  17.9 kDa
ORF:  8118 .. 8531  =  414 bp
ORF:  137 amino acids  =  14.8 kDa
ORF:  3515 .. 3973  =  459 bp
ORF:  152 amino acids  =  16.7 kDa
ORF:  5408 .. 5803  =  396 bp
ORF:  131 amino acids  =  14.7 kDa
ORF:  8027 .. 8323  =  297 bp
ORF:  98 amino acids  =  10.5 kDa
ORF:  1819 .. 2259  =  441 bp
ORF:  146 amino acids  =  16.0 kDa
ORF:  3331 .. 3681  =  351 bp
ORF:  116 amino acids  =  12.2 kDa
ORF:  4609 .. 5196  =  588 bp
ORF:  195 amino acids  =  20.7 kDa
ORF:  5965 .. 6231  =  267 bp
ORF:  88 amino acids  =  9.8 kDa
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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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