pGreenII

Improved compact Agrobacterium binary vector with a kanamycin-resistance gene. Also known as pGreenII 0000.

Sequence Author: pGreen website

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HomePlasmidsPlant VectorspGreenII
BsaBI * (3275) DraIII (3087) BsrDI (3063) BspHI (3031) NruI (2896) TsoI (2714) PasI (2678) EcoNI (2640) SspI (2628) AsiSI (2555) Bpu10I - BsmBI (2533) PflMI (2293) BspHI (2070) AcuI (1898) AlwNI (1766) ApaLI (1664) PspFI (1658) BseYI (1654) StyI (330) NgoMIV (433) NaeI (435) FseI (437) AanI - AanI - PsiI (455) BglII (536) HpaI (568) FspI (612) BmrI (713) Acc65I (783) KpnI (787) PspOMI (789) EcoO109I (790) ApaI (793) AbsI - PaeR7I - PspXI - XhoI (798) SalI (804) AccI (805) BspDI - ClaI (814) HindIII (819) EcoRV (827) EcoRI (831) PstI (841) TspMI - XmaI (843) SmaI (845) BamHI (849) SpeI (855) XbaI (861) NotI (868) BpmI (873) AleI - MslI (879) SacII (880) BstXI (881) Eco53kI (887) SacI (889) lac operator BspQI - SapI (1168) StuI (1292) BglII (1345) DrdI (1458) BciVI (1553) pGreenII 3304 bp
BsaBI  (3275)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
DraIII  (3087)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsrDI  (3063)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
BspHI  (3031)
2 sites		 T C A T G A A G T A C T
NruI  (2896)
1 site		 T C G C G A A G C G C T
TsoI  (2714)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PasI  (2678)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
EcoNI  (2640)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SspI  (2628)
1 site		 A A T A T T T T A T A A
AsiSI  (2555)
1 site		 G C G A T C G C C G C T A G C G
Bpu10I  (2533)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (2533)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
PflMI  (2293)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BspHI  (2070)
2 sites		 T C A T G A A G T A C T
AcuI  (1898)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (1766)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (1664)
1 site		 G T G C A C C A C G T G
PspFI  (1658)
1 site		 C C C A G C G G G T C G
BseYI  (1654)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
StyI  (330)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
NgoMIV  (433)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (435)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
FseI  (437)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
AanI  (455)
1 site		 T T A T A A A A T A T T
AanI  (455)
1 site		 T T A T A A A A T A T T
PsiI  (455)
1 site		 T T A T A A A A T A T T
BglII  (536)
2 sites		 A G A T C T T C T A G A
HpaI  (568)
1 site		 G T T A A C C A A T T G
FspI  (612)
1 site		 T G C G C A A C G C G T
BmrI  (713)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
Acc65I  (783)
1 site		 G G T A C C C C A T G G
KpnI  (787)
1 site		 G G T A C C C C A T G G
PspOMI  (789)
1 site		 G G G C C C C C C G G G
EcoO109I  (790)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
ApaI  (793)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
AbsI  (798)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (798)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (798)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (798)
1 site		 C T C G A G G A G C T C
SalI  (804)
1 site		 G T C G A C C A G C T G
AccI  (805)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BspDI  (814)
1 site		 A T C G A T T A G C T A
ClaI  (814)
1 site		 A T C G A T T A G C T A
HindIII  (819)
1 site		 A A G C T T T T C G A A
EcoRV  (827)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
EcoRI  (831)
1 site		 G A A T T C C T T A A G
PstI  (841)
1 site		 C T G C A G G A C G T C
TspMI  (843)
1 site		 C C C G G G G G G C C C
XmaI  (843)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (845)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (849)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SpeI  (855)
1 site		 A C T A G T T G A T C A
XbaI  (861)
1 site		 T C T A G A A G A T C T
NotI  (868)
1 site		 G C G G C C G C C G C C G G C G
BpmI  (873)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AleI  (879)
1 site		 C A C N N N N G T G G T G N N N N C A C
MslI  (879)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
SacII  (880)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstXI  (881)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
Eco53kI  (887)
1 site		 G A G C T C C T C G A G
SacI  (889)
1 site		 G A G C T C C T C G A G
BspQI  (1168)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1168)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
StuI  (1292)
1 site		 A G G C C T T C C G G A
BglII  (1345)
2 sites		 A G A T C T T C T A G A
DrdI  (1458)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BciVI  (1553)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
KanR
2170 .. 2985  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
2170 .. 2985  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
lacZα
296 .. 946  =  651 bp
216 amino acids  =  23.7 kDa
Product: lacZα fragment of β-galactosidase
lacZα
296 .. 946  =  651 bp
216 amino acids  =  23.7 kDa
Product: lacZα fragment of β-galactosidase
ori
1411 .. 1999  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1411 .. 1999  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
lac promoter
990 .. 1021  =  32 bp
3 segments
   Segment 3:  -10  
   990 .. 996  =  7 bp
promoter for the E. coli lac operon
lac promoter
990 .. 1021  =  32 bp
3 segments
   Segment 2:  
   997 .. 1015  =  19 bp
promoter for the E. coli lac operon
lac promoter
990 .. 1021  =  32 bp
3 segments
   Segment 1:  -35  
   1016 .. 1021  =  6 bp
promoter for the E. coli lac operon
lac promoter
990 .. 1021  =  32 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
1296 .. 1320  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
1296 .. 1320  =  25 bp
right border repeat from nopaline C58 T-DNA
lac operator
966 .. 982  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
966 .. 982  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
pSa ori
3276 .. 407  =  436 bp
origin of replication from bacterial plasmid pSa
pSa ori
3276 .. 407  =  436 bp
origin of replication from bacterial plasmid pSa
MCS
783 .. 890  =  108 bp
pBluescript multiple cloning site
MCS
783 .. 890  =  108 bp
pBluescript multiple cloning site
LB T-DNA repeat
542 .. 564  =  23 bp
left border repeat from nopaline C58 T-DNA (truncated)
LB T-DNA repeat
542 .. 564  =  23 bp
left border repeat from nopaline C58 T-DNA (truncated)
T7 promoter
756 .. 774  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
756 .. 774  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T3 promoter
903 .. 921  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T3 promoter
903 .. 921  =  19 bp
promoter for bacteriophage T3 RNA polymerase
M13 fwd
733 .. 749  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
733 .. 749  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
942 .. 958  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
942 .. 958  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  1332 .. 1736  =  405 bp
ORF:  134 amino acids  =  15.0 kDa
ORF:  2148 .. 2375  =  228 bp
ORF:  75 amino acids  =  8.8 kDa
ORF:  296 .. 946  =  651 bp
ORF:  216 amino acids  =  23.7 kDa
ORF:  2170 .. 2985  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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