pRI 101-AN

Agrobacterium binary vector for foreign gene expression in dicotyledonous plants.

Sequence Author: TaKaRa

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HomePlasmidsPlant VectorspRI 101-AN
PmeI (9133) HindIII (9008) SphI (9006) PstI - SbfI (9000) BsaI (8913) ScaI (8783) PshAI (8340) XbaI (8143) AtADH 5'-UTR NdeI (8080) SalI (8070) SmaI (8066) TspMI - XmaI (8064) KpnI (8062) Acc65I (8058) BamHI (8052) EcoRI (8046) SacI (7987) Eco53kI (7985) M13 rev lac operator lac promoter CAP binding site NOS promoter FspI (6982) BsaAI (6780) ApaI (6151) PspOMI (6147) PspXI (5493) BbvCI (5292) ApaLI (310) BstEII (1838) BstAPI (2018) PflMI (2086) BclI * (2394) AgeI (4300) AvrII (5073) pRI 101-AN 10,417 bp
PmeI  (9133)
1 site		 G T T T A A A C C A A A T T T G
HindIII  (9008)
1 site		 A A G C T T T T C G A A
SphI  (9006)
1 site		 G C A T G C C G T A C G
PstI  (9000)
1 site		 C T G C A G G A C G T C
SbfI  (9000)
1 site		 C C T G C A G G G G A C G T C C
BsaI  (8913)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ScaI  (8783)
1 site		 A G T A C T T C A T G A
PshAI  (8340)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
XbaI  (8143)
1 site		 T C T A G A A G A T C T
NdeI  (8080)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SalI  (8070)
1 site		 G T C G A C C A G C T G
SmaI  (8066)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (8064)
1 site		 C C C G G G G G G C C C
XmaI  (8064)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
KpnI  (8062)
1 site		 G G T A C C C C A T G G
Acc65I  (8058)
1 site		 G G T A C C C C A T G G
BamHI  (8052)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (8046)
1 site		 G A A T T C C T T A A G
SacI  (7987)
1 site		 G A G C T C C T C G A G
Eco53kI  (7985)
1 site		 G A G C T C C T C G A G
FspI  (6982)
1 site		 T G C G C A A C G C G T
BsaAI  (6780)
1 site		 Y A C G T R R T G C A Y
ApaI  (6151)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (6147)
1 site		 G G G C C C C C C G G G
PspXI  (5493)
1 site		 V C T C G A G B B G A G C T C V
BbvCI  (5292)
1 site		 C C T C A G C G G A G T C G
ApaLI  (310)
1 site		 G T G C A C C A C G T G
BstEII  (1838)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstAPI  (2018)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
PflMI  (2086)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BclI  (2394)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AgeI  (4300)
1 site		 A C C G G T T G G C C A
AvrII  (5073)
1 site		 C C T A G G G G A T C C
pRi replicator region
889 .. 5524  =  4636 bp
replicator region from Agrobacterium plasmid pRiA 4b
pRi replicator region
889 .. 5524  =  4636 bp
replicator region from Agrobacterium plasmid pRiA 4b
NeoR/KanR
6419 .. 7213  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
6419 .. 7213  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
KanR
9256 .. 10,050  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
9256 .. 10,050  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
57 .. 645  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
57 .. 645  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CaMV 35S promoter
8157 .. 8502  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
8157 .. 8502  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
NOS terminator
5893 .. 6145  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
5893 .. 6145  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
7720 .. 7972  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
7720 .. 7972  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
7270 .. 7449  =  180 bp
nopaline synthase promoter
NOS promoter
7270 .. 7449  =  180 bp
nopaline synthase promoter
MCS
7983 .. 8084  =  102 bp
multiple cloning site
MCS
7983 .. 8084  =  102 bp
multiple cloning site
AtADH 5'-UTR
8085 .. 8142  =  58 bp
translational enhancer from the 5'-UTR of the Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et al., 2008
AtADH 5'-UTR
8085 .. 8142  =  58 bp
translational enhancer from the 5'-UTR of the Arabidopsis thaliana alcohol dehydrogenase gene (Sugio et al., 2008
lac promoter
7620 .. 7650  =  31 bp
3 segments
   Segment 1:  -35  
   7620 .. 7625  =  6 bp
promoter for the E. coli lac operon
lac promoter
7620 .. 7650  =  31 bp
3 segments
   Segment 2:  
   7626 .. 7643  =  18 bp
promoter for the E. coli lac operon
lac promoter
7620 .. 7650  =  31 bp
3 segments
   Segment 3:  -10  
   7644 .. 7650  =  7 bp
promoter for the E. coli lac operon
lac promoter
7620 .. 7650  =  31 bp
3 segments
promoter for the E. coli lac operon
LB T-DNA repeat
5531 .. 5555  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
5531 .. 5555  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9148 .. 9172  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
9148 .. 9172  =  25 bp
right border repeat from nopaline C58 T-DNA
CAP binding site
7584 .. 7605  =  22 bp
CAP binding activates transcription in the presence of cAMP.
CAP binding site
7584 .. 7605  =  22 bp
CAP binding activates transcription in the presence of cAMP.
lac operator
7658 .. 7674  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
7658 .. 7674  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
M13 rev
7682 .. 7698  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
7682 .. 7698  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  2422 .. 3444  =  1023 bp
ORF:  340 amino acids  =  37.6 kDa
ORF:  4879 .. 5142  =  264 bp
ORF:  87 amino acids  =  9.7 kDa
ORF:  1211 .. 2425  =  1215 bp
ORF:  404 amino acids  =  45.0 kDa
ORF:  6368 .. 6904  =  537 bp
ORF:  178 amino acids  =  19.9 kDa
ORF:  9389 .. 9619  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  3597 .. 4814  =  1218 bp
ORF:  405 amino acids  =  44.3 kDa
ORF:  5142 .. 5633  =  492 bp
ORF:  163 amino acids  =  18.0 kDa
ORF:  6075 .. 6368  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  10,410 .. 382  =  390 bp
ORF:  129 amino acids  =  14.4 kDa
ORF:  3467 .. 3742  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  4202 .. 4513  =  312 bp
ORF:  103 amino acids  =  10.9 kDa
ORF:  6419 .. 7213  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  8732 .. 8962  =  231 bp
ORF:  76 amino acids  =  9.1 kDa
ORF:  3148 .. 3387  =  240 bp
ORF:  79 amino acids  =  9.2 kDa
ORF:  3721 .. 4092  =  372 bp
ORF:  123 amino acids  =  13.9 kDa
ORF:  6655 .. 7041  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
ORF:  9256 .. 10,050  =  795 bp
ORF:  264 amino acids  =  31.0 kDa
ORF:  6051 .. 6398  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  8256 .. 9005  =  750 bp
ORF:  249 amino acids  =  28.0 kDa
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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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