pSB1

Superbinary acceptor vector for plant cell transformation, with a tetracycline-resistance gene.
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HomePlasmidsPlant VectorspSB1
KpnI (45) AvrII (20,349) MauBI (19,473) virD1 SnaBI (5139) FseI (8462) BsiWI (11,188) KpnI (14,854) BstZ17I (15,048) PciI (15,275) SbfI (16,851) pSB1 36,909 bp
KpnI  (45)
2 sites		 G G T A C C C C A T G G
AvrII  (20,349)
1 site		 C C T A G G G G A T C C
MauBI  (19,473)
1 site		 C G C G C G C G G C G C G C G C
SnaBI  (5139)
1 site		 T A C G T A A T G C A T
FseI  (8462)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
BsiWI  (11,188)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
KpnI  (14,854)
2 sites		 G G T A C C C C A T G G
BstZ17I  (15,048)
1 site		 G T A T A C C A T A T G
PciI  (15,275)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
SbfI  (16,851)
1 site		 C C T G C A G G G G A C G T C C
incC,korABFG operon
31,395 .. 34,828  =  3434 bp
incC,korABFG operon
31,395 .. 34,828  =  3434 bp
virB4
9057 .. 11,426  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
virB4
9057 .. 11,426  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
kfrBC operon
28,439 .. 30,352  =  1914 bp
kfrBC operon
28,439 .. 30,352  =  1914 bp
TcR
19,088 .. 20,287  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
19,088 .. 20,287  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
trfA
21,560 .. 22,708  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
trfA
21,560 .. 22,708  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
virB10
4487 .. 5620  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB10
4487 .. 5620  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB11
3416 .. 4447  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB11
3416 .. 4447  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB6
7396 .. 8283  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virB6
7396 .. 8283  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virG
2481 .. 3284  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component transduction system
virG
2481 .. 3284  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component transduction system
virB1
12,132 .. 12,851  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB1
12,132 .. 12,851  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB8
6495 .. 7208  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
virB8
6495 .. 7208  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
oriV
35,754 .. 36,465  =  712 bp
incP origin of replication
oriV
35,754 .. 36,465  =  712 bp
incP origin of replication
virC1 (inactivated)
1065 .. 1759  =  695 bp
Product: VirC1, inactivated by a frameshift mutation that eliminated the final C in codon Thr-56
virC1 (inactivated)
1065 .. 1759  =  695 bp
Product: VirC1, inactivated by a frameshift mutation that eliminated the final C in codon Thr-56
virB5
8380 .. 9042  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
virB5
8380 .. 9042  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
TetR
18,332 .. 18,982  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
18,332 .. 18,982  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
virC2
1762 .. 2370  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
virC2
1762 .. 2370  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
ori
15,336 .. 15,924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
15,336 .. 15,924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
virD1
353 .. 796  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
virD1
353 .. 796  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
cos
16,432 .. 16,830  =  399 bp
lambda cos site; allows packaging into phage lambda particles
cos
16,432 .. 16,830  =  399 bp
lambda cos site; allows packaging into phage lambda particles
traJ
26,118 .. 26,489  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
26,118 .. 26,489  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
virB2
11,752 .. 12,117  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
virB2
11,752 .. 12,117  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
bom
15,010 .. 15,150  =  141 bp
basis of mobility region from pBR322
bom
15,010 .. 15,150  =  141 bp
basis of mobility region from pBR322
oriT
26,522 .. 26,631  =  110 bp
incP origin of transfer
oriT
26,522 .. 26,631  =  110 bp
incP origin of transfer
kfrA operon
30,253 .. 31,335  =  1083 bp
kfrA operon
30,253 .. 31,335  =  1083 bp
virB9
5617 .. 6498  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB9
5617 .. 6498  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB3
11,426 .. 11,752  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB3
11,426 .. 11,752  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB7
7195 .. 7362  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
virB7
7195 .. 7362  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
ORF:  1762 .. 2370  =  609 bp
ORF:  202 amino acids  =  22.4 kDa
ORF:  2662 .. 3294  =  633 bp
ORF:  210 amino acids  =  23.3 kDa
ORF:  4837 .. 5082  =  246 bp
ORF:  81 amino acids  =  9.5 kDa
ORF:  5491 .. 5718  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  8521 .. 8799  =  279 bp
ORF:  92 amino acids  =  10.6 kDa
ORF:  9559 .. 9813  =  255 bp
ORF:  84 amino acids  =  9.1 kDa
ORF:  11,674 .. 11,898  =  225 bp
ORF:  74 amino acids  =  8.0 kDa
ORF:  13,138 .. 13,518  =  381 bp
ORF:  126 amino acids  =  14.6 kDa
ORF:  16,225 .. 16,536  =  312 bp
ORF:  103 amino acids  =  11.0 kDa
ORF:  25,276 .. 25,587  =  312 bp
ORF:  103 amino acids  =  12.1 kDa
ORF:  26,857 .. 27,261  =  405 bp
ORF:  134 amino acids  =  14.7 kDa
ORF:  28,936 .. 29,199  =  264 bp
ORF:  87 amino acids  =  9.0 kDa
ORF:  29,506 .. 29,811  =  306 bp
ORF:  101 amino acids  =  10.6 kDa
ORF:  34,261 .. 34,488  =  228 bp
ORF:  75 amino acids  =  8.2 kDa
ORF:  1202 .. 1759  =  558 bp
ORF:  185 amino acids  =  20.9 kDa
ORF:  2147 .. 2449  =  303 bp
ORF:  100 amino acids  =  11.5 kDa
ORF:  4856 .. 5332  =  477 bp
ORF:  158 amino acids  =  17.5 kDa
ORF:  11,798 .. 12,382  =  585 bp
ORF:  194 amino acids  =  21.7 kDa
ORF:  13,301 .. 13,633  =  333 bp
ORF:  110 amino acids  =  12.4 kDa
ORF:  16,826 .. 17,062  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  19,013 .. 20,287  =  1275 bp
ORF:  424 amino acids  =  45.1 kDa
ORF:  21,572 .. 21,967  =  396 bp
ORF:  131 amino acids  =  14.7 kDa
ORF:  23,282 .. 23,593  =  312 bp
ORF:  103 amino acids  =  11.3 kDa
ORF:  23,927 .. 24,823  =  897 bp
ORF:  298 amino acids  =  32.5 kDa
ORF:  24,836 .. 25,273  =  438 bp
ORF:  145 amino acids  =  15.0 kDa
ORF:  25,448 .. 25,747  =  300 bp
ORF:  99 amino acids  =  11.2 kDa
ORF:  26,990 .. 27,457  =  468 bp
ORF:  155 amino acids  =  17.4 kDa
ORF:  27,983 .. 28,420  =  438 bp
ORF:  145 amino acids  =  15.6 kDa
ORF:  28,436 .. 28,693  =  258 bp
ORF:  85 amino acids  =  8.6 kDa
ORF:  31,730 .. 32,185  =  456 bp
ORF:  151 amino acids  =  16.0 kDa
ORF:  32,192 .. 32,488  =  297 bp
ORF:  98 amino acids  =  9.9 kDa
ORF:  33,455 .. 33,682  =  228 bp
ORF:  75 amino acids  =  8.4 kDa
ORF:  33,773 .. 34,000  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  35,519 .. 36,031  =  513 bp
ORF:  170 amino acids  =  17.9 kDa
ORF:  1065 .. 1394  =  330 bp
ORF:  109 amino acids  =  11.8 kDa
ORF:  11,214 .. 11,558  =  345 bp
ORF:  114 amino acids  =  12.5 kDa
ORF:  13,038 .. 13,262  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  13,947 .. 14,324  =  378 bp
ORF:  125 amino acids  =  14.4 kDa
ORF:  21,144 .. 21,410  =  267 bp
ORF:  88 amino acids  =  9.8 kDa
ORF:  22,179 .. 22,766  =  588 bp
ORF:  195 amino acids  =  20.7 kDa
ORF:  25,584 .. 26,105  =  522 bp
ORF:  173 amino acids  =  18.7 kDa
ORF:  27,261 .. 27,986  =  726 bp
ORF:  241 amino acids  =  26.6 kDa
ORF:  32,730 .. 33,245  =  516 bp
ORF:  171 amino acids  =  18.0 kDa
ORF:  367 .. 591  =  225 bp
ORF:  74 amino acids  =  8.7 kDa
ORF:  1447 .. 1836  =  390 bp
ORF:  129 amino acids  =  13.9 kDa
ORF:  5617 .. 6498  =  882 bp
ORF:  293 amino acids  =  32.1 kDa
ORF:  7396 .. 8316  =  921 bp
ORF:  306 amino acids  =  33.1 kDa
ORF:  8380 .. 9042  =  663 bp
ORF:  220 amino acids  =  23.5 kDa
ORF:  11,752 .. 12,117  =  366 bp
ORF:  121 amino acids  =  12.3 kDa
ORF:  19,441 .. 19,791  =  351 bp
ORF:  116 amino acids  =  12.3 kDa
ORF:  21,847 .. 22,119  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  22,705 .. 23,004  =  300 bp
ORF:  99 amino acids  =  11.0 kDa
ORF:  23,884 .. 24,693  =  810 bp
ORF:  269 amino acids  =  29.0 kDa
ORF:  27,931 .. 28,377  =  447 bp
ORF:  148 amino acids  =  15.4 kDa
ORF:  29,848 .. 30,195  =  348 bp
ORF:  115 amino acids  =  12.7 kDa
ORF:  31,465 .. 31,992  =  528 bp
ORF:  175 amino acids  =  19.6 kDa
ORF:  32,002 .. 32,505  =  504 bp
ORF:  167 amino acids  =  17.5 kDa
ORF:  33,649 .. 34,770  =  1122 bp
ORF:  373 amino acids  =  39.2 kDa
ORF:  34,951 .. 35,271  =  321 bp
ORF:  106 amino acids  =  12.2 kDa
ORF:  35,458 .. 35,760  =  303 bp
ORF:  100 amino acids  =  9.4 kDa
ORF:  765 .. 989  =  225 bp
ORF:  74 amino acids  =  8.2 kDa
ORF:  2481 .. 3284  =  804 bp
ORF:  267 amino acids  =  30.0 kDa
ORF:  5736 .. 6074  =  339 bp
ORF:  112 amino acids  =  13.1 kDa
ORF:  6495 .. 7268  =  774 bp
ORF:  257 amino acids  =  28.4 kDa
ORF:  9057 .. 11,426  =  2370 bp
ORF:  789 amino acids  =  87.4 kDa
ORF:  12,132 .. 12,851  =  720 bp
ORF:  239 amino acids  =  25.9 kDa
ORF:  12,939 .. 13,187  =  249 bp
ORF:  82 amino acids  =  9.5 kDa
ORF:  13,470 .. 13,706  =  237 bp
ORF:  78 amino acids  =  9.1 kDa
ORF:  14,328 .. 14,675  =  348 bp
ORF:  115 amino acids  =  12.8 kDa
ORF:  16,095 .. 16,406  =  312 bp
ORF:  103 amino acids  =  11.1 kDa
ORF:  16,824 .. 17,393  =  570 bp
ORF:  189 amino acids  =  20.8 kDa
ORF:  20,319 .. 21,305  =  987 bp
ORF:  328 amino acids  =  34.9 kDa
ORF:  21,585 .. 21,980  =  396 bp
ORF:  131 amino acids  =  14.1 kDa
ORF:  25,083 .. 25,331  =  249 bp
ORF:  82 amino acids  =  9.4 kDa
ORF:  25,524 .. 25,898  =  375 bp
ORF:  124 amino acids  =  13.5 kDa
ORF:  26,118 .. 26,489  =  372 bp
ORF:  123 amino acids  =  13.5 kDa
ORF:  27,045 .. 27,743  =  699 bp
ORF:  232 amino acids  =  24.8 kDa
ORF:  28,473 .. 29,558  =  1086 bp
ORF:  361 amino acids  =  39.9 kDa
ORF:  31,176 .. 31,520  =  345 bp
ORF:  114 amino acids  =  12.4 kDa
ORF:  34,056 .. 34,301  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  34,425 .. 34,793  =  369 bp
ORF:  122 amino acids  =  13.8 kDa
ORF:  34,803 .. 35,072  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  36,309 .. 36,536  =  228 bp
ORF:  75 amino acids  =  8.3 kDa
ORF:  2 .. 319  =  318 bp
ORF:  105 amino acids  =  11.8 kDa
ORF:  353 .. 796  =  444 bp
ORF:  147 amino acids  =  16.3 kDa
ORF:  3416 .. 4447  =  1032 bp
ORF:  343 amino acids  =  38.0 kDa
ORF:  4487 .. 5716  =  1230 bp
ORF:  409 amino acids  =  44.1 kDa
ORF:  8960 .. 9184  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  10,142 .. 10,450  =  309 bp
ORF:  102 amino acids  =  11.0 kDa
ORF:  11,426 .. 11,752  =  327 bp
ORF:  108 amino acids  =  11.8 kDa
ORF:  13,763 .. 13,990  =  228 bp
ORF:  75 amino acids  =  8.3 kDa
ORF:  18,332 .. 19,009  =  678 bp
ORF:  225 amino acids  =  24.3 kDa
ORF:  21,560 .. 22,708  =  1149 bp
ORF:  382 amino acids  =  43.8 kDa
ORF:  22,757 .. 23,107  =  351 bp
ORF:  116 amino acids  =  13.1 kDa
ORF:  23,684 .. 24,595  =  912 bp
ORF:  303 amino acids  =  33.6 kDa
ORF:  27,554 .. 27,808  =  255 bp
ORF:  84 amino acids  =  9.2 kDa
ORF:  27,836 .. 28,162  =  327 bp
ORF:  108 amino acids  =  11.8 kDa
ORF:  28,562 .. 28,807  =  246 bp
ORF:  81 amino acids  =  9.2 kDa
ORF:  29,027 .. 29,299  =  273 bp
ORF:  90 amino acids  =  10.5 kDa
ORF:  30,353 .. 31,279  =  927 bp
ORF:  308 amino acids  =  34.0 kDa
ORF:  32,576 .. 33,652  =  1077 bp
ORF:  358 amino acids  =  39.0 kDa
ORF:  34,904 .. 35,461  =  558 bp
ORF:  185 amino acids  =  19.4 kDa
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Download pSB1.dna file

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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

Plasmid Sets

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I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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