pCAMBIA5105

Plant vector containing vir operons from pTiBo542, compatible with a range of non-Agrobacterium bacteria.

Sequence Author: Cambia

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CaMV poly(A) signal lac promoter lac operator SwaI (47,077) AvrII (47,068) SbfI (47,065) CaMV 35S promoter SpeI (46,076) 6xHis RB T-DNA repeat pVS1 oriV bom M13 ori M13 fwd PmeI (29,070) LB T-DNA repeat pCAMBIA5105 49,757 bp
SwaI  (47,077)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AvrII  (47,068)
1 site
C C T A G G G G A T C C
SbfI  (47,065)
1 site
C C T G C A G G G G A C G T C C
SpeI  (46,076)
1 site
A C T A G T T G A T C A
PmeI  (29,070)
1 site
G T T T A A A C C A A A T T T G
virD5
26,569 .. 29,079  =  2511 bp
836 amino acids  =  93.3 kDa
Product: VirD5
virD5
26,569 .. 29,079  =  2511 bp
836 amino acids  =  93.3 kDa
Product: VirD5
virA
2014 .. 4503  =  2490 bp
829 amino acids  =  91.5 kDa
Product: VirA
sensory component of two-component signal transduction system
virA
2014 .. 4503  =  2490 bp
829 amino acids  =  91.5 kDa
Product: VirA
sensory component of two-component signal transduction system
virB4
11,473 .. 13,842  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
virB4
11,473 .. 13,842  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
6xHis
44,249 .. 44,266  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
44,249 .. 44,266  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
   Segment 2:  
   44,273 .. 46,090  =  1818 bp
   606 amino acids  =  69.2 kDa
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
   Segment 1:  
   46,281 .. 46,295  =  15 bp
   5 amino acids  =  632.8 Da
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
GUSPlus™
44,273 .. 46,295  =  2023 bp
611 amino acids  =  69.8 kDa
2 segments  =  1833 bp
Product: β-glucuronidase
codon-optimized Staphylococcus gusA gene with a catalase intron to ensure expression in plants but not bacteria
virE3
31,447 .. 33,465  =  2019 bp
672 amino acids  =  75.7 kDa
Product: VirE3
virE3
31,447 .. 33,465  =  2019 bp
672 amino acids  =  75.7 kDa
Product: VirE3
virD4
24,510 .. 26,468  =  1959 bp
652 amino acids  =  72.1 kDa
Product: VirD4
coupling protein
virD4
24,510 .. 26,468  =  1959 bp
652 amino acids  =  72.1 kDa
Product: VirD4
coupling protein
virE2
29,731 .. 31,380  =  1650 bp
549 amino acids  =  62.6 kDa
Product: VirE2
single-stranded DNA binding protein
virE2
29,731 .. 31,380  =  1650 bp
549 amino acids  =  62.6 kDa
Product: VirE2
single-stranded DNA binding protein
virD2
22,581 .. 23,855  =  1275 bp
424 amino acids  =  47.5 kDa
Product: VirD2
relaxase
virD2
22,581 .. 23,855  =  1275 bp
424 amino acids  =  47.5 kDa
Product: VirD2
relaxase
virB10
17,279 .. 18,412  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB10
17,279 .. 18,412  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
pVS1 RepA
40,488 .. 41,561  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
pVS1 RepA
40,488 .. 41,561  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
virB11
18,452 .. 19,483  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB11
18,452 .. 19,483  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
HygR
48,176 .. 49,201  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
HygR
48,176 .. 49,201  =  1026 bp
341 amino acids  =  38.0 kDa
Product: hygromycin B phosphotransferase
confers resistance to hygromycin
virB6
14,616 .. 15,503  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virB6
14,616 .. 15,503  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virG
19,615 .. 20,418  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component transduction system
virG
19,615 .. 20,418  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component transduction system
NeoR/KanR
36,190 .. 36,984  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
36,190 .. 36,984  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
SmR
37,935 .. 38,726  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase
confers resistance to spectinomycin and streptomycin
SmR
37,935 .. 38,726  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase
confers resistance to spectinomycin and streptomycin
virJ
7828 .. 8571  =  744 bp
247 amino acids  =  27.2 kDa
Product: VirJ
virJ
7828 .. 8571  =  744 bp
247 amino acids  =  27.2 kDa
Product: VirJ
virB1
10,048 .. 10,767  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB1
10,048 .. 10,767  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB8
15,691 .. 16,404  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
virB8
15,691 .. 16,404  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
virC1
21,140 .. 21,835  =  696 bp
231 amino acids  =  25.6 kDa
Product: VirC1
virC1
21,140 .. 21,835  =  696 bp
231 amino acids  =  25.6 kDa
Product: VirC1
CaMV 35S promoter (enhanced)
47,431 .. 48,108  =  678 bp
cauliflower mosaic virus 35S promoter with a duplicated enhancer region
CaMV 35S promoter (enhanced)
47,431 .. 48,108  =  678 bp
cauliflower mosaic virus 35S promoter with a duplicated enhancer region
virB5
13,857 .. 14,519  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
virB5
13,857 .. 14,519  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
pVS1 StaA
41,990 .. 42,619  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
pVS1 StaA
41,990 .. 42,619  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
virC2
20,529 .. 21,137  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
virC2
20,529 .. 21,137  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
ori
38,970 .. 39,558  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
38,970 .. 39,558  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
virD1
22,104 .. 22,547  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
virD1
22,104 .. 22,547  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
virK
262 .. 699  =  438 bp
145 amino acids  =  15.8 kDa
Product: VirK
virK
262 .. 699  =  438 bp
145 amino acids  =  15.8 kDa
Product: VirK
M13 ori
35,442 .. 35,822  =  381 bp
M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
M13 ori
35,442 .. 35,822  =  381 bp
M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
virB2
10,782 .. 11,147  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
virB2
10,782 .. 11,147  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
CaMV 35S promoter
46,312 .. 46,657  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
46,312 .. 46,657  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
NOS terminator
43,965 .. 44,217  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
43,965 .. 44,217  =  253 bp
nopaline synthase terminator and poly(A) signal
lacZα
46,891 .. 47,130  =  240 bp
79 amino acids  =  8.7 kDa
Product: LacZα fragment of β-galactosidase
lacZα
46,891 .. 47,130  =  240 bp
79 amino acids  =  8.7 kDa
Product: LacZα fragment of β-galactosidase
virE1
29,529 .. 29,726  =  198 bp
65 amino acids  =  7.3 kDa
Product: VirE1
chaperone for VirE2
virE1
29,529 .. 29,726  =  198 bp
65 amino acids  =  7.3 kDa
Product: VirE1
chaperone for VirE2
pVS1 oriV
40,228 .. 40,422  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
pVS1 oriV
40,228 .. 40,422  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
CaMV poly(A) signal
49,241 .. 49,415  =  175 bp
cauliflower mosaic virus polyadenylation signal
CaMV poly(A) signal
49,241 .. 49,415  =  175 bp
cauliflower mosaic virus polyadenylation signal
bom
39,744 .. 39,884  =  141 bp
basis of mobility region from pBR322
bom
39,744 .. 39,884  =  141 bp
basis of mobility region from pBR322
lac promoter
47,174 .. 47,204  =  31 bp
3 segments
   Segment 3:  -10  
   47,174 .. 47,180  =  7 bp
promoter for the E. coli lac operon
lac promoter
47,174 .. 47,204  =  31 bp
3 segments
   Segment 2:  
   47,181 .. 47,198  =  18 bp
promoter for the E. coli lac operon
lac promoter
47,174 .. 47,204  =  31 bp
3 segments
   Segment 1:  -35  
   47,199 .. 47,204  =  6 bp
promoter for the E. coli lac operon
lac promoter
47,174 .. 47,204  =  31 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
43,919 .. 43,943  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
43,919 .. 43,943  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
49,493 .. 49,517  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
49,493 .. 49,517  =  25 bp
left border repeat from nopaline C58 T-DNA
T7 promoter
35,260 .. 35,278  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
35,260 .. 35,278  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
35,285 .. 35,301  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
35,285 .. 35,301  =  17 bp
common sequencing primer, one of multiple similar variants
lac operator
47,150 .. 47,166  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
47,150 .. 47,166  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
virB9
16,401 .. 17,282  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB9
16,401 .. 17,282  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virD3
23,875 .. 24,513  =  639 bp
212 amino acids  =  22.7 kDa
Product: VirD3
virD3
23,875 .. 24,513  =  639 bp
212 amino acids  =  22.7 kDa
Product: VirD3
virB3
11,147 .. 11,473  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB3
11,147 .. 11,473  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
cat1 intron
46,091 .. 46,280  =  190 bp
castor bean catalase intron, modified
cat1 intron
46,091 .. 46,280  =  190 bp
castor bean catalase intron, modified
virB7
15,537 .. 15,704  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
virB7
15,537 .. 15,704  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
M13 fwd
47,034 .. 47,050  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
47,034 .. 47,050  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
47,126 .. 47,142  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
47,126 .. 47,142  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  262 .. 699  =  438 bp
ORF:  145 amino acids  =  15.8 kDa
ORF:  1291 .. 1557  =  267 bp
ORF:  88 amino acids  =  9.6 kDa
ORF:  2014 .. 4503  =  2490 bp
ORF:  829 amino acids  =  91.5 kDa
ORF:  5557 .. 5901  =  345 bp
ORF:  114 amino acids  =  12.9 kDa
ORF:  6826 .. 7122  =  297 bp
ORF:  98 amino acids  =  10.5 kDa
ORF:  7828 .. 8571  =  744 bp
ORF:  247 amino acids  =  27.2 kDa
ORF:  9193 .. 9429  =  237 bp
ORF:  78 amino acids  =  9.1 kDa
ORF:  9712 .. 9960  =  249 bp
ORF:  82 amino acids  =  9.5 kDa
ORF:  10,048 .. 10,767  =  720 bp
ORF:  239 amino acids  =  25.9 kDa
ORF:  11,473 .. 13,842  =  2370 bp
ORF:  789 amino acids  =  87.4 kDa
ORF:  15,631 .. 16,404  =  774 bp
ORF:  257 amino acids  =  28.4 kDa
ORF:  16,825 .. 17,163  =  339 bp
ORF:  112 amino acids  =  13.1 kDa
ORF:  19,615 .. 20,418  =  804 bp
ORF:  267 amino acids  =  30.0 kDa
ORF:  22,309 .. 22,533  =  225 bp
ORF:  74 amino acids  =  8.7 kDa
ORF:  23,164 .. 23,463  =  300 bp
ORF:  99 amino acids  =  11.2 kDa
ORF:  23,674 .. 24,513  =  840 bp
ORF:  279 amino acids  =  30.2 kDa
ORF:  26,569 .. 29,079  =  2511 bp
ORF:  836 amino acids  =  93.3 kDa
ORF:  29,731 .. 31,380  =  1650 bp
ORF:  549 amino acids  =  62.6 kDa
ORF:  31,447 .. 33,465  =  2019 bp
ORF:  672 amino acids  =  75.7 kDa
ORF:  33,877 .. 34,230  =  354 bp
ORF:  117 amino acids  =  13.1 kDa
ORF:  36,190 .. 36,984  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  41,458 .. 41,715  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  43,222 .. 43,572  =  351 bp
ORF:  116 amino acids  =  12.6 kDa
ORF:  3794 .. 4357  =  564 bp
ORF:  187 amino acids  =  21.5 kDa
ORF:  5174 .. 5560  =  387 bp
ORF:  128 amino acids  =  14.0 kDa
ORF:  5645 .. 5920  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  8909 .. 9136  =  228 bp
ORF:  75 amino acids  =  8.3 kDa
ORF:  11,147 .. 11,473  =  327 bp
ORF:  108 amino acids  =  11.8 kDa
ORF:  12,449 .. 12,757  =  309 bp
ORF:  102 amino acids  =  11.0 kDa
ORF:  13,715 .. 13,939  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  17,183 .. 18,412  =  1230 bp
ORF:  409 amino acids  =  44.1 kDa
ORF:  18,452 .. 19,483  =  1032 bp
ORF:  343 amino acids  =  38.0 kDa
ORF:  21,911 .. 22,135  =  225 bp
ORF:  74 amino acids  =  8.2 kDa
ORF:  27,251 .. 27,628  =  378 bp
ORF:  125 amino acids  =  13.7 kDa
ORF:  28,268 .. 28,585  =  318 bp
ORF:  105 amino acids  =  11.5 kDa
ORF:  29,927 .. 30,253  =  327 bp
ORF:  108 amino acids  =  11.9 kDa
ORF:  33,056 .. 33,352  =  297 bp
ORF:  98 amino acids  =  11.7 kDa
ORF:  34,676 .. 34,909  =  234 bp
ORF:  77 amino acids  =  8.8 kDa
ORF:  35,438 .. 35,692  =  255 bp
ORF:  84 amino acids  =  8.7 kDa
ORF:  36,362 .. 36,748  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  42,881 .. 43,153  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
ORF:  44,147 .. 46,096  =  1950 bp
ORF:  649 amino acids  =  73.4 kDa
ORF:  47,219 .. 47,677  =  459 bp
ORF:  152 amino acids  =  16.9 kDa
ORF:  47,687 .. 48,004  =  318 bp
ORF:  105 amino acids  =  11.6 kDa
ORF:  48,176 .. 49,201  =  1026 bp
ORF:  341 amino acids  =  38.0 kDa
ORF:  96 .. 353  =  258 bp
ORF:  85 amino acids  =  9.8 kDa
ORF:  1629 .. 1922  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  5976 .. 7565  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  10,782 .. 11,147  =  366 bp
ORF:  121 amino acids  =  12.3 kDa
ORF:  13,857 .. 14,519  =  663 bp
ORF:  220 amino acids  =  23.5 kDa
ORF:  14,583 .. 15,503  =  921 bp
ORF:  306 amino acids  =  33.1 kDa
ORF:  16,401 .. 17,282  =  882 bp
ORF:  293 amino acids  =  32.1 kDa
ORF:  21,063 .. 21,452  =  390 bp
ORF:  129 amino acids  =  13.9 kDa
ORF:  22,104 .. 22,547  =  444 bp
ORF:  147 amino acids  =  16.3 kDa
ORF:  22,581 .. 23,855  =  1275 bp
ORF:  424 amino acids  =  47.5 kDa
ORF:  24,510 .. 26,468  =  1959 bp
ORF:  652 amino acids  =  72.1 kDa
ORF:  33,399 .. 33,734  =  336 bp
ORF:  111 amino acids  =  12.9 kDa
ORF:  34,227 .. 34,520  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  35,139 .. 35,465  =  327 bp
ORF:  108 amino acids  =  12.2 kDa
ORF:  37,002 .. 37,622  =  621 bp
ORF:  206 amino acids  =  23.0 kDa
ORF:  37,794 .. 38,726  =  933 bp
ORF:  310 amino acids  =  34.5 kDa
ORF:  41,121 .. 41,693  =  573 bp
ORF:  190 amino acids  =  20.6 kDa
ORF:  42,567 .. 42,881  =  315 bp
ORF:  104 amino acids  =  12.0 kDa
ORF:  44,682 .. 44,909  =  228 bp
ORF:  75 amino acids  =  7.7 kDa
ORF:  4077 .. 4304  =  228 bp
ORF:  75 amino acids  =  8.0 kDa
ORF:  6180 .. 6533  =  354 bp
ORF:  117 amino acids  =  13.6 kDa
ORF:  6816 .. 7058  =  243 bp
ORF:  80 amino acids  =  8.2 kDa
ORF:  7263 .. 7535  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
ORF:  9381 .. 9761  =  381 bp
ORF:  126 amino acids  =  14.6 kDa
ORF:  11,001 .. 11,225  =  225 bp
ORF:  74 amino acids  =  8.0 kDa
ORF:  13,086 .. 13,340  =  255 bp
ORF:  84 amino acids  =  9.1 kDa
ORF:  14,100 .. 14,378  =  279 bp
ORF:  92 amino acids  =  10.6 kDa
ORF:  17,181 .. 17,408  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  17,817 .. 18,062  =  246 bp
ORF:  81 amino acids  =  9.5 kDa
ORF:  19,605 .. 20,237  =  633 bp
ORF:  210 amino acids  =  23.3 kDa
ORF:  20,529 .. 21,137  =  609 bp
ORF:  202 amino acids  =  22.4 kDa
ORF:  23,091 .. 23,471  =  381 bp
ORF:  126 amino acids  =  14.3 kDa
ORF:  28,767 .. 29,009  =  243 bp
ORF:  80 amino acids  =  8.9 kDa
ORF:  29,454 .. 29,681  =  228 bp
ORF:  75 amino acids  =  8.8 kDa
ORF:  31,176 .. 31,448  =  273 bp
ORF:  90 amino acids  =  10.1 kDa
ORF:  34,404 .. 34,661  =  258 bp
ORF:  85 amino acids  =  9.7 kDa
ORF:  34,998 .. 35,237  =  240 bp
ORF:  79 amino acids  =  9.5 kDa
ORF:  36,252 .. 36,611  =  360 bp
ORF:  119 amino acids  =  12.5 kDa
ORF:  37,974 .. 38,357  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  40,488 .. 41,663  =  1176 bp
ORF:  391 amino acids  =  43.9 kDa
ORF:  42,918 .. 43,604  =  687 bp
ORF:  228 amino acids  =  25.1 kDa
ORF:  4670 .. 4921  =  252 bp
ORF:  83 amino acids  =  9.6 kDa
ORF:  5015 .. 5356  =  342 bp
ORF:  113 amino acids  =  13.1 kDa
ORF:  5876 .. 6538  =  663 bp
ORF:  220 amino acids  =  23.3 kDa
ORF:  7046 .. 7339  =  294 bp
ORF:  97 amino acids  =  10.7 kDa
ORF:  9266 .. 9598  =  333 bp
ORF:  110 amino acids  =  12.4 kDa
ORF:  10,517 .. 11,101  =  585 bp
ORF:  194 amino acids  =  21.7 kDa
ORF:  17,567 .. 18,043  =  477 bp
ORF:  158 amino acids  =  17.5 kDa
ORF:  20,450 .. 20,752  =  303 bp
ORF:  100 amino acids  =  11.5 kDa
ORF:  21,140 .. 21,835  =  696 bp
ORF:  231 amino acids  =  25.6 kDa
ORF:  23,267 .. 23,563  =  297 bp
ORF:  98 amino acids  =  10.0 kDa
ORF:  24,293 .. 24,553  =  261 bp
ORF:  86 amino acids  =  9.7 kDa
ORF:  27,662 .. 28,000  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  34,670 .. 35,155  =  486 bp
ORF:  161 amino acids  =  17.4 kDa
ORF:  37,967 .. 38,224  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  38,234 .. 38,512  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  41,990 .. 42,619  =  630 bp
ORF:  209 amino acids  =  22.1 kDa
ORF:  44,243 .. 45,949  =  1707 bp
ORF:  568 amino acids  =  64.7 kDa
ORF:  48,509 .. 49,249  =  741 bp
ORF:  246 amino acids  =  27.0 kDa
ORF:  748 .. 1455  =  708 bp
ORF:  235 amino acids  =  26.4 kDa
ORF:  5206 .. 5595  =  390 bp
ORF:  129 amino acids  =  13.9 kDa
ORF:  8575 .. 8952  =  378 bp
ORF:  125 amino acids  =  14.4 kDa
ORF:  9637 .. 9861  =  225 bp
ORF:  74 amino acids  =  8.1 kDa
ORF:  11,341 .. 11,685  =  345 bp
ORF:  114 amino acids  =  12.5 kDa
ORF:  29,881 .. 30,312  =  432 bp
ORF:  143 amino acids  =  16.3 kDa
ORF:  31,441 .. 31,707  =  267 bp
ORF:  88 amino acids  =  10.1 kDa
ORF:  34,222 .. 34,785  =  564 bp
ORF:  187 amino acids  =  20.1 kDa
ORF:  36,499 .. 36,753  =  255 bp
ORF:  84 amino acids  =  9.5 kDa
ORF:  46,411 .. 46,839  =  429 bp
ORF:  142 amino acids  =  15.8 kDa
ORF:  46,891 .. 47,130  =  240 bp
ORF:  79 amino acids  =  8.7 kDa
Click here to try SnapGene

Download pCAMBIA5105.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

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