pGA643

Binary Ti vector for expression of foreign genes in plant cells.
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HomePlasmidsPlant VectorspGA643
PmeI (39) NotI (11,010) PasI (10,458) AgeI (9831) ApaI (8048) PspOMI (8044) BstXI (7685) StuI (7446) AvrII (7396) FspAI (6736) BtsI - BtsαI (6261) CsiI - SexAI * (6168) AleI (6080) Bsu36I (247) BamHI (408) PstI (573) AflII (1840) HindIII (2824) XbaI (2829) HpaI (2841) MCS BglII (2857) gene 7 terminator BstZ17I (3092) EcoRI (3620) AhdI (4009) SgrDI (4112) SfiI (5396) NdeI (5954) pGA643 11,601 bp
PmeI  (39)
1 site		 G T T T A A A C C A A A T T T G
NotI  (11,010)
1 site		 G C G G C C G C C G C C G G C G
PasI  (10,458)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
AgeI  (9831)
1 site		 A C C G G T T G G C C A
ApaI  (8048)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (8044)
1 site		 G G G C C C C C C G G G
BstXI  (7685)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
StuI  (7446)
1 site		 A G G C C T T C C G G A
AvrII  (7396)
1 site		 C C T A G G G G A T C C
FspAI  (6736)
1 site		 R T G C G C A Y Y A C G C G T R
BtsI  (6261)
1 site		 G C A G T G N N C G T C A C
BtsαI  (6261)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
CsiI  (6168)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (6168)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AleI  (6080)
1 site		 C A C N N N N G T G G T G N N N N C A C
Bsu36I  (247)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BamHI  (408)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PstI  (573)
1 site		 C T G C A G G A C G T C
AflII  (1840)
1 site		 C T T A A G G A A T T C
HindIII  (2824)
1 site		 A A G C T T T T C G A A
XbaI  (2829)
1 site		 T C T A G A A G A T C T
HpaI  (2841)
1 site		 G T T A A C C A A T T G
BglII  (2857)
1 site		 A G A T C T T C T A G A
BstZ17I  (3092)
1 site		 G T A T A C C A T A T G
EcoRI  (3620)
1 site		 G A A T T C C T T A A G
AhdI  (4009)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SgrDI  (4112)
1 site		 C G T C G A C G G C A G C T G C
SfiI  (5396)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
NdeI  (5954)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
TcR
7463 .. 8662  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
7463 .. 8662  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
trfA
5042 .. 6190  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
trfA
5042 .. 6190  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
KanR
365 .. 1186  =  822 bp
273 amino acids  =  29.9 kDa
3 segments
   Segment 1:  nos  
   365 .. 406  =  42 bp
   14 amino acids  =  1.4 kDa
Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
365 .. 1186  =  822 bp
273 amino acids  =  29.9 kDa
3 segments
   Segment 2:  
   407 .. 415  =  9 bp
   3 amino acids  =  443.5 Da
Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
365 .. 1186  =  822 bp
273 amino acids  =  29.9 kDa
3 segments
   Segment 3:  nptII  
   416 .. 1186  =  771 bp
   256 amino acids  =  28.1 kDa
Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
365 .. 1186  =  822 bp
273 amino acids  =  29.9 kDa
3 segments
Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase
confers resistance to kanamycin
TetR
8768 .. 9418  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
8768 .. 9418  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
oriV
10,483 .. 11,114  =  632 bp
incP origin of replication
oriV
10,483 .. 11,114  =  632 bp
incP origin of replication
traJ
4399 .. 4770  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
4399 .. 4770  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
CaMV 35S promoter
2471 .. 2815  =  345 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
2471 .. 2815  =  345 bp
strong constitutive promoter from cauliflower mosaic virus
NOS terminator
1811 .. 2063  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
1811 .. 2063  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
149 .. 364  =  216 bp
nopaline synthase promoter
NOS promoter
149 .. 364  =  216 bp
nopaline synthase promoter
gene 7 terminator
2875 .. 3075  =  201 bp
transcription terminator for octopine-type Ti plasmid gene 7
gene 7 terminator
2875 .. 3075  =  201 bp
transcription terminator for octopine-type Ti plasmid gene 7
oriT
4257 .. 4366  =  110 bp
incP origin of transfer
oriT
4257 .. 4366  =  110 bp
incP origin of transfer
MCS
2824 .. 2862  =  39 bp
multiple cloning site
MCS
2824 .. 2862  =  39 bp
multiple cloning site
RB T-DNA repeat
1 .. 25  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
1 .. 25  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
3678 .. 3702  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
3678 .. 3702  =  25 bp
left border repeat from nopaline C58 T-DNA
ORF:  4399 .. 4770  =  372 bp
ORF:  123 amino acids  =  13.5 kDa
ORF:  5770 .. 6165  =  396 bp
ORF:  131 amino acids  =  14.1 kDa
ORF:  6445 .. 7431  =  987 bp
ORF:  328 amino acids  =  34.9 kDa
ORF:  365 .. 1186  =  822 bp
ORF:  273 amino acids  =  29.9 kDa
ORF:  5042 .. 6190  =  1149 bp
ORF:  382 amino acids  =  43.8 kDa
ORF:  8741 .. 9418  =  678 bp
ORF:  225 amino acids  =  24.3 kDa
ORF:  564 .. 950  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  1554 .. 1790  =  237 bp
ORF:  78 amino acids  =  8.5 kDa
ORF:  2379 .. 2717  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  5631 .. 5903  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  7959 .. 8309  =  351 bp
ORF:  116 amino acids  =  12.3 kDa
ORF:  9864 .. 10,094  =  231 bp
ORF:  76 amino acids  =  9.0 kDa
ORF:  4984 .. 5571  =  588 bp
ORF:  195 amino acids  =  20.7 kDa
ORF:  6340 .. 6606  =  267 bp
ORF:  88 amino acids  =  9.8 kDa
ORF:  9450 .. 10,346  =  897 bp
ORF:  298 amino acids  =  34.1 kDa
ORF:  701 .. 1237  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  5783 .. 6178  =  396 bp
ORF:  131 amino acids  =  14.7 kDa
ORF:  7463 .. 8737  =  1275 bp
ORF:  424 amino acids  =  45.2 kDa
ORF:  10,961 .. 11,212  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
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Download pGA643.dna file

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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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