pEarleyGate 301

Gateway®-compatible promoterless plant transformation vector with a C-terminal HA tag.

Sequence Author: Pikaard Lab

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HomePlasmidsPlant VectorspEarleyGate 301
AseI (94) BclI * (10,142) PsiI (10,011) BspHI (9685) EcoRV (9262) NsiI (9151) PluTI (8003) SfoI (8001) NarI (8000) KasI (7999) EcoNI (7527) BseRI (7491) BmtI (7218) NheI (7214) BsiWI (7098) XmnI (95) Eco53kI (103) SacI (105) FspAI (523) PaqCI (587) AfeI (760) attR1 BspEI (1806) NcoI (2111) MluI (2257) BbvCI (2535) SrfI (2681) BstXI (2798) HA XbaI (3109) PacI (3122) SpeI (3134) MfeI (3438) BstEII (3710) AhdI (3840) SbfI (3865) HindIII (3873) PvuI (3999) PmeI (4086) pEarleyGate 301 10,379 bp
AseI  (94)
1 site		 A T T A A T T A A T T A
BclI  (10,142)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PsiI  (10,011)
1 site		 T T A T A A A A T A T T
BspHI  (9685)
1 site		 T C A T G A A G T A C T
EcoRV  (9262)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NsiI  (9151)
1 site		 A T G C A T T A C G T A
PluTI  (8003)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (8001)
1 site		 G G C G C C C C G C G G
NarI  (8000)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (7999)
1 site		 G G C G C C C C G C G G
EcoNI  (7527)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BseRI  (7491)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BmtI  (7218)
1 site		 G C T A G C C G A T C G
NheI  (7214)
1 site		 G C T A G C C G A T C G
BsiWI  (7098)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
XmnI  (95)
1 site		 G A A N N N N T T C C T T N N N N A A G
Eco53kI  (103)
1 site		 G A G C T C C T C G A G
SacI  (105)
1 site		 G A G C T C C T C G A G
FspAI  (523)
1 site		 R T G C G C A Y Y A C G C G T R
PaqCI  (587)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
AfeI  (760)
1 site		 A G C G C T T C G C G A
BspEI  (1806)
1 site		 T C C G G A A G G C C T
NcoI  (2111)
1 site		 C C A T G G G G T A C C
MluI  (2257)
1 site		 A C G C G T T G C G C A
BbvCI  (2535)
1 site		 C C T C A G C G G A G T C G
SrfI  (2681)
1 site		 G C C C G G G C C G G G C C C G
BstXI  (2798)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
XbaI  (3109)
1 site		 T C T A G A A G A T C T
PacI  (3122)
1 site		 T T A A T T A A A A T T A A T T
SpeI  (3134)
1 site		 A C T A G T T G A T C A
MfeI  (3438)
1 site		 C A A T T G G T T A A C
BstEII  (3710)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AhdI  (3840)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (3865)
1 site		 C C T G C A G G G G A C G T C C
HindIII  (3873)
1 site		 A A G C T T T T C G A A
PvuI  (3999)
1 site		 C G A T C G G C T A G C
PmeI  (4086)
1 site		 G T T T A A A C C A A A T T T G
pVS1 RepA
6483 .. 7556  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
pVS1 RepA
6483 .. 7556  =  1074 bp
357 amino acids  =  39.9 kDa
Product: replication protein from Pseudomonas plasmid pVS1
KanR
9161 .. 9955  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
9161 .. 9955  =  795 bp
264 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
OCS terminator
3150 .. 3857  =  708 bp
octopine synthase terminator
OCS terminator
3150 .. 3857  =  708 bp
octopine synthase terminator
CmR
1597 .. 2256  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1597 .. 2256  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
pVS1 StaA
5425 .. 6054  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
pVS1 StaA
5425 .. 6054  =  630 bp
209 amino acids  =  22.1 kDa
Product: stability protein from Pseudomonas plasmid pVS1
ori
8486 .. 9074  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
8486 .. 9074  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
BlpR
375 .. 926  =  552 bp
183 amino acids  =  20.6 kDa
Product: phosphinothricin acetyltransferase
confers resistance to bialophos or phosphinothricin
BlpR
375 .. 926  =  552 bp
183 amino acids  =  20.6 kDa
Product: phosphinothricin acetyltransferase
confers resistance to bialophos or phosphinothricin
MAS promoter
932 .. 1312  =  381 bp
mannopine synthase promoter (Velten et al., 1984)
MAS promoter
932 .. 1312  =  381 bp
mannopine synthase promoter (Velten et al., 1984)
ccdB
2598 .. 2903  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
2598 .. 2903  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
MAS terminator
113 .. 365  =  253 bp
mannopine synthase terminator
MAS terminator
113 .. 365  =  253 bp
mannopine synthase terminator
pVS1 oriV
7622 .. 7816  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
pVS1 oriV
7622 .. 7816  =  195 bp
origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)
bom
8160 .. 8300  =  141 bp
basis of mobility region from pBR322
bom
8160 .. 8300  =  141 bp
basis of mobility region from pBR322
attR2
2944 .. 3068  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2944 .. 3068  =  125 bp
recombination site for the Gateway® LR reaction
attR1
1364 .. 1483  =  120 bp
recombination site for the Gateway® LR reaction
attR1
1364 .. 1483  =  120 bp
recombination site for the Gateway® LR reaction
lac UV5 promoter
1513 .. 1543  =  31 bp
3 segments
   Segment 1:  -35  
   1513 .. 1518  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1513 .. 1543  =  31 bp
3 segments
   Segment 2:  
   1519 .. 1536  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1513 .. 1543  =  31 bp
3 segments
   Segment 3:  -10  
   1537 .. 1543  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1513 .. 1543  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
HA
3070 .. 3096  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
3070 .. 3096  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
LB T-DNA repeat
3 .. 27  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
3 .. 27  =  25 bp
left border repeat from nopaline C58 T-DNA
RB T-DNA repeat
4101 .. 4125  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
4101 .. 4125  =  25 bp
right border repeat from nopaline C58 T-DNA
ORF:  277 .. 609  =  333 bp
ORF:  110 amino acids  =  11.9 kDa
ORF:  1597 .. 2256  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  5425 .. 6054  =  630 bp
ORF:  209 amino acids  =  22.1 kDa
ORF:  3737 .. 4027  =  291 bp
ORF:  96 amino acids  =  10.1 kDa
ORF:  450 .. 959  =  510 bp
ORF:  169 amino acids  =  18.0 kDa
ORF:  2598 .. 2903  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  4440 .. 5126  =  687 bp
ORF:  228 amino acids  =  25.1 kDa
ORF:  6381 .. 7556  =  1176 bp
ORF:  391 amino acids  =  43.9 kDa
ORF:  9294 .. 9524  =  231 bp
ORF:  76 amino acids  =  8.3 kDa
ORF:  10,277 .. 323  =  426 bp
ORF:  141 amino acids  =  15.5 kDa
ORF:  375 .. 926  =  552 bp
ORF:  183 amino acids  =  20.6 kDa
ORF:  5163 .. 5477  =  315 bp
ORF:  104 amino acids  =  12.0 kDa
ORF:  6351 .. 6923  =  573 bp
ORF:  190 amino acids  =  20.6 kDa
ORF:  4472 .. 4822  =  351 bp
ORF:  116 amino acids  =  12.6 kDa
ORF:  6329 .. 6586  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  9161 .. 9955  =  795 bp
ORF:  264 amino acids  =  31.0 kDa
ORF:  4891 .. 5163  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
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Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

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