pCAMBIA1391Xa
Agrobacterium binary vector with hygromycin- and kanamycin-resistance and GUS genes plus the pUC9 MCS. For other reading frames, use pCAMBIA1391Xb or pCAMBIA1391Xc.
Sequence Author: Cambia
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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ApaI can be used between 25°C and 37°C. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different PpuMI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different PasI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
6xHis 1832 .. 1849 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
6xHis 1832 .. 1849 = 18 bp 6 amino acids = 840.9 Da Product: 6xHis affinity tag |
pVS1 RepA 4537 .. 5610 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
pVS1 RepA 4537 .. 5610 = 1074 bp 357 amino acids = 39.9 kDa Product: replication protein from Pseudomonas plasmid pVS1 |
HygR 8750 .. 9775 = 1026 bp 341 amino acids = 38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
HygR 8750 .. 9775 = 1026 bp 341 amino acids = 38.0 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
KanR 7215 .. 8009 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
KanR 7215 .. 8009 = 795 bp 264 amino acids = 31.0 kDa Product: aminoglycoside phosphotransferase confers resistance to kanamycin |
CaMV 35S promoter (enhanced) 9843 .. 10,520 = 678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
CaMV 35S promoter (enhanced) 9843 .. 10,520 = 678 bp cauliflower mosaic virus 35S promoter with a duplicated enhancer region |
pVS1 StaA 3479 .. 4108 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
pVS1 StaA 3479 .. 4108 = 630 bp 209 amino acids = 22.1 kDa Product: stability protein from Pseudomonas plasmid pVS1 |
ori 6540 .. 7128 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 6540 .. 7128 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
NOS terminator 1881 .. 2133 = 253 bp nopaline synthase terminator and poly(A) signal |
NOS terminator 1881 .. 2133 = 253 bp nopaline synthase terminator and poly(A) signal |
pVS1 oriV 5676 .. 5870 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
pVS1 oriV 5676 .. 5870 = 195 bp origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000) |
CaMV poly(A) signal 8536 .. 8710 = 175 bp cauliflower mosaic virus polyadenylation signal |
CaMV poly(A) signal 8536 .. 8710 = 175 bp cauliflower mosaic virus polyadenylation signal |
bom 6214 .. 6354 = 141 bp basis of mobility region from pBR322 |
bom 6214 .. 6354 = 141 bp basis of mobility region from pBR322 |
MCS 10,609 .. 10,644 = 36 bp multiple cloning site from pUC9 |
MCS 10,609 .. 10,644 = 36 bp multiple cloning site from pUC9 |
RB T-DNA repeat 2155 .. 2179 = 25 bp right border repeat from nopaline C58 T-DNA |
RB T-DNA repeat 2155 .. 2179 = 25 bp right border repeat from nopaline C58 T-DNA |
LB T-DNA repeat 8434 .. 8458 = 25 bp left border repeat from nopaline C58 T-DNA |
LB T-DNA repeat 8434 .. 8458 = 25 bp left border repeat from nopaline C58 T-DNA |
ORF: 2494 .. 3180 = 687 bp ORF: 228 amino acids = 25.1 kDa |
ORF: 4435 .. 5610 = 1176 bp ORF: 391 amino acids = 43.9 kDa |
ORF: 7348 .. 7578 = 231 bp ORF: 76 amino acids = 8.3 kDa |
ORF: 329 .. 1855 = 1527 bp ORF: 508 amino acids = 57.4 kDa |
ORF: 3479 .. 4108 = 630 bp ORF: 209 amino acids = 22.1 kDa |
ORF: 8702 .. 9442 = 741 bp ORF: 246 amino acids = 27.0 kDa |
ORF: 2526 .. 2876 = 351 bp ORF: 116 amino acids = 12.6 kDa |
ORF: 4383 .. 4640 = 258 bp ORF: 85 amino acids = 9.6 kDa |
ORF: 7215 .. 8009 = 795 bp ORF: 264 amino acids = 31.0 kDa |
ORF: 131 .. 592 = 462 bp ORF: 153 amino acids = 17.5 kDa |
ORF: 1181 .. 1507 = 327 bp ORF: 108 amino acids = 12.8 kDa |
ORF: 1559 .. 1951 = 393 bp ORF: 130 amino acids = 14.5 kDa |
ORF: 2945 .. 3217 = 273 bp ORF: 90 amino acids = 9.9 kDa |
ORF: 8750 .. 9775 = 1026 bp ORF: 341 amino acids = 38.0 kDa |
ORF: 9947 .. 10,264 = 318 bp ORF: 105 amino acids = 11.6 kDa |
ORF: 10,274 .. 10,591 = 318 bp ORF: 105 amino acids = 11.6 kDa |
ORF: 3217 .. 3531 = 315 bp ORF: 104 amino acids = 12.0 kDa |
ORF: 4405 .. 4977 = 573 bp ORF: 190 amino acids = 20.6 kDa |
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GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
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GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
|
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
GUS 20 .. 1825 = 1806 bp 602 amino acids = 68.3 kDa Product: β-glucuronidase |
Click here to try SnapGene |
Download pCAMBIA1391Xa.dna file
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