pGreen 0029

Compact Agrobacterium binary vector with a kanamycin-resistance genes for bacterial and plant transformation.

Sequence Author: pGreen website

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
No matches
HomePlasmidsPlant VectorspGreen 0029
RsrII (4505) AflII (4285) AflIII - PciI (4205) MauBI (4091) SphI (4061) PsiI (3937) FseI (3919) StyI (3812) DraIII (3265) BspHI (3209) NruI (3074) EcoNI (2818) AsiSI (2733) Bpu10I - BsmBI - Esp3I (2711) PflMI (2471) NmeAIII (170) PflFI - Tth111I (273) MscI (310) KasI (389) BsaHI - NarI (390) SfoI (391) PluTI (393) NheI (616) BmtI (620) Bsu36I (632) BlpI (677) Acc65I (1031) KpnI (1035) PspOMI (1037) EcoO109I (1038) ApaI (1041) AbsI - PaeR7I - PspXI - XhoI (1046) SalI (1052) AccI (1053) HincII (1054) HindIII (1067) EcoRV (1075) EcoRI (1079) PstI (1089) TspMI - XmaI (1091) SmaI (1093) BamHI (1097) SpeI (1103) XbaI (1109) NotI (1116) AleI - MslI (1127) BstXI (1129) Eco53kI (1135) SacI (1137) lac operator StuI (1540) ApaLI (1912) AlwNI (2014) pGreen 0029 4632 bp
RsrII  (4505)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AflII  (4285)
1 site		 C T T A A G G A A T T C
AflIII  (4205)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (4205)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
MauBI  (4091)
1 site		 C G C G C G C G G C G C G C G C
SphI  (4061)
1 site		 G C A T G C C G T A C G
PsiI  (3937)
1 site		 T T A T A A A A T A T T
FseI  (3919)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
StyI  (3812)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
DraIII  (3265)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BspHI  (3209)
1 site		 T C A T G A A G T A C T
NruI  (3074)
1 site		 T C G C G A A G C G C T
EcoNI  (2818)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AsiSI  (2733)
1 site		 G C G A T C G C C G C T A G C G
Bpu10I  (2711)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (2711)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2711)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PflMI  (2471)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
NmeAIII  (170)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PflFI  (273)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (273)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (310)
1 site		 T G G C C A A C C G G T
KasI  (389)
1 site		 G G C G C C C C G C G G
BsaHI  (390)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (390)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (391)
1 site		 G G C G C C C C G C G G
PluTI  (393)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
NheI  (616)
1 site		 G C T A G C C G A T C G
BmtI  (620)
1 site		 G C T A G C C G A T C G
Bsu36I  (632)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BlpI  (677)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
Acc65I  (1031)
1 site		 G G T A C C C C A T G G
KpnI  (1035)
1 site		 G G T A C C C C A T G G
PspOMI  (1037)
1 site		 G G G C C C C C C G G G
EcoO109I  (1038)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
ApaI  (1041)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
AbsI  (1046)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (1046)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1046)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (1046)
1 site		 C T C G A G G A G C T C
SalI  (1052)
1 site		 G T C G A C C A G C T G
AccI  (1053)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (1054)
1 site		 G T Y R A C C A R Y T G
HindIII  (1067)
1 site		 A A G C T T T T C G A A
EcoRV  (1075)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
EcoRI  (1079)
1 site		 G A A T T C C T T A A G
PstI  (1089)
1 site		 C T G C A G G A C G T C
TspMI  (1091)
1 site		 C C C G G G G G G C C C
XmaI  (1091)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1093)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (1097)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SpeI  (1103)
1 site		 A C T A G T T G A T C A
XbaI  (1109)
1 site		 T C T A G A A G A T C T
NotI  (1116)
1 site		 G C G G C C G C C G C C G G C G
AleI  (1127)
1 site		 C A C N N N N G T G G T G N N N N C A C
MslI  (1127)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
BstXI  (1129)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
Eco53kI  (1135)
1 site		 G A G C T C C T C G A G
SacI  (1137)
1 site		 G A G C T C C T C G A G
StuI  (1540)
1 site		 A G G C C T T C C G G A
ApaLI  (1912)
1 site		 G T G C A C C A C G T G
AlwNI  (2014)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
KanR
2348 .. 3163  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
2348 .. 3163  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
NeoR/KanR
4359 .. 521  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4359 .. 521  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
1659 .. 2247  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1659 .. 2247  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
pSa ori
3454 .. 3889  =  436 bp
origin of replication from bacterial plasmid pSa
pSa ori
3454 .. 3889  =  436 bp
origin of replication from bacterial plasmid pSa
lacZα
805 .. 1194  =  390 bp
129 amino acids  =  14.2 kDa
Product: LacZα fragment of β-galactosidase
lacZα
805 .. 1194  =  390 bp
129 amino acids  =  14.2 kDa
Product: LacZα fragment of β-galactosidase
NOS terminator
4068 .. 4319  =  252 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
4068 .. 4319  =  252 bp
nopaline synthase terminator and poly(A) signal
NOS promoter
555 .. 734  =  180 bp
nopaline synthase promoter
NOS promoter
555 .. 734  =  180 bp
nopaline synthase promoter
lac promoter
1238 .. 1269  =  32 bp
3 segments
   Segment 3:  -10  
   1238 .. 1244  =  7 bp
promoter for the E. coli lac operon
lac promoter
1238 .. 1269  =  32 bp
3 segments
   Segment 2:  
   1245 .. 1263  =  19 bp
promoter for the E. coli lac operon
lac promoter
1238 .. 1269  =  32 bp
3 segments
   Segment 1:  -35  
   1264 .. 1269  =  6 bp
promoter for the E. coli lac operon
lac promoter
1238 .. 1269  =  32 bp
3 segments
promoter for the E. coli lac operon
RB T-DNA repeat
1544 .. 1568  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
1544 .. 1568  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
4022 .. 4044  =  23 bp
left border repeat from nopaline C58 T-DNA (truncated)
LB T-DNA repeat
4022 .. 4044  =  23 bp
left border repeat from nopaline C58 T-DNA (truncated)
lac operator
1214 .. 1230  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1214 .. 1230  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
MCS
1031 .. 1138  =  108 bp
pBluescript multiple cloning site
MCS
1031 .. 1138  =  108 bp
pBluescript multiple cloning site
T7 promoter
1004 .. 1022  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1004 .. 1022  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T3 promoter
1151 .. 1169  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T3 promoter
1151 .. 1169  =  19 bp
promoter for bacteriophage T3 RNA polymerase
M13 fwd
981 .. 997  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
981 .. 997  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
1190 .. 1206  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
1190 .. 1206  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  1580 .. 1984  =  405 bp
ORF:  134 amino acids  =  15.0 kDa
ORF:  3828 .. 4082  =  255 bp
ORF:  84 amino acids  =  9.5 kDa
ORF:  805 .. 1194  =  390 bp
ORF:  129 amino acids  =  14.2 kDa
ORF:  3778 .. 4080  =  303 bp
ORF:  100 amino acids  =  11.0 kDa
ORF:  4359 .. 521  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  4595 .. 349  =  387 bp
ORF:  128 amino acids  =  14.5 kDa
ORF:  2348 .. 3163  =  816 bp
ORF:  271 amino acids  =  30.9 kDa
Click here to try SnapGene

Download pGreen 0029.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pBI121
pCAMBIA1381Xa
pEarleyGate 201
pPZP100
pBI221
pCAMBIA1381Xb
pEarleyGate 202
pPZP101
pBin19
pCAMBIA1381Xc
pEarleyGate 203
pPZP102
pBINPLUS
pCAMBIA1381Z
pEarleyGate 204
pPZP111
pCAMBIA0105.1R
pCAMBIA1390
pEarleyGate 205
pPZP112
pCAMBIA0305.1
pCAMBIA1391
pEarleyGate 301
pPZP121
pCAMBIA0305.2
pCAMBIA1391Xa
pEarleyGate 302
pPZP122
pCAMBIA0380
pCAMBIA1391Xb
pEarleyGate 303
pPZP200
pCAMBIA0390
pCAMBIA1391Xc
pEarleyGate 304
pPZP201
pCAMBIA1105.1
pCAMBIA1391Z
pFGC5941
pPZP202
pCAMBIA1105.1R
pCAMBIA2200
pGA643
pPZP211
pCAMBIA1200
pCAMBIA2201
pGreen
pPZP212
pCAMBIA1201
pCAMBIA2300
pGreen 0029
pPZP221
pCAMBIA1281Z
pCAMBIA2301
pGreenII
pPZP222
pCAMBIA1291Z
pCAMBIA3200
pGreenII 0049
pRI 101-AN
pCAMBIA1300
pCAMBIA3201
pGreenII 0179
pRI 101-ON
pCAMBIA1301
pCAMBIA3300
pGreenII 0229
pRI 201-AN
pCAMBIA1302
pCAMBIA3301
pGreenII 0579
pRI 201-ON
pCAMBIA1303
pCAMBIA5105
pHANNIBAL
pRI 909
pCAMBIA1304
pEarleyGate 100
pHELLSGATE
pRI 910
pCAMBIA1305.1
pEarleyGate 101
pHELLSGATE 12
pSB1
pCAMBIA1305.2
pEarleyGate 102
pHELLSGATE 4
pSB11
pCAMBIA1380
pEarleyGate 103
pHELLSGATE 8
pSoup
pCAMBIA1381
pEarleyGate 104
pKANNIBAL

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free