pAAV-AC-Myc-DDK

Adeno-associated virus (AAV) vector for expressing a C-terminally myc- and FLAG-tagged protein using the CMV promoter.

Sequence Author: OriGene

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HomePlasmidsViral Expression & Packaging VectorspAAV-AC-Myc-DDK
PciI (4749) NmeAIII (3714) FspI (3638) ScaI (3380) XmnI (3261) PsiI (2590) DraIII (2465) NdeI (390) SnaBI (496) NcoI (516) Eco53kI (722) SacI (724) SacII (811) BsaBI (817) BGI-F sequencing primer (1173 .. 1192) BspDI * - ClaI * (1317) BseRI (1339) AsiSI (1344) MreI - SgrAI (1346) AscI (1350) HindIII (1364) NheI (1375) BmtI (1379) MluI (1387) BsiWI (1390) PaeR7I - PspXI - XhoI (1402) EcoRV (1446) PmeI (1480) FseI (1490) AleI (1558) XL39-R sequencing primer (1564 .. 1583) BsgI (1742) BbvCI - Bpu10I (1784) SphI (1817) NsiI (1819) BlpI (1828) BstEII (1989) PmlI (1997) KasI (2158) NarI (2159) SfoI (2160) PluTI (2162) pAAV-AC-Myc-DDK 4754 bp
PciI  (4749)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
NmeAIII  (3714)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
FspI  (3638)
1 site		 T G C G C A A C G C G T
ScaI  (3380)
1 site		 A G T A C T T C A T G A
XmnI  (3261)
1 site		 G A A N N N N T T C C T T N N N N A A G
PsiI  (2590)
1 site		 T T A T A A A A T A T T
DraIII  (2465)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
NdeI  (390)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (496)
1 site		 T A C G T A A T G C A T
NcoI  (516)
1 site		 C C A T G G G G T A C C
Eco53kI  (722)
1 site		 G A G C T C C T C G A G
SacI  (724)
1 site		 G A G C T C C T C G A G
SacII  (811)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
BsaBI  (817)
1 site		 G A T N N N N A T C C T A N N N N T A G
BspDI  (1317)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1317)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BseRI  (1339)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AsiSI  (1344)
1 site		 G C G A T C G C C G C T A G C G
MreI  (1346)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (1346)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AscI  (1350)
1 site		 G G C G C G C C C C G C G C G G
HindIII  (1364)
1 site		 A A G C T T T T C G A A
NheI  (1375)
1 site		 G C T A G C C G A T C G
BmtI  (1379)
1 site		 G C T A G C C G A T C G
MluI  (1387)
1 site		 A C G C G T T G C G C A
BsiWI  (1390)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
PaeR7I  (1402)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1402)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (1402)
1 site		 C T C G A G G A G C T C
EcoRV  (1446)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PmeI  (1480)
1 site		 G T T T A A A C C A A A T T T G
FseI  (1490)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
AleI  (1558)
1 site		 C A C N N N N G T G G T G N N N N C A C
BsgI  (1742)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbvCI  (1784)
1 site		 C C T C A G C G G A G T C G
Bpu10I  (1784)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
SphI  (1817)
1 site		 G C A T G C C G T A C G
NsiI  (1819)
1 site		 A T G C A T T A C G T A
BlpI  (1828)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BstEII  (1989)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PmlI  (1997)
1 site		 C A C G T G G T G C A C
KasI  (2158)
1 site		 G G C G C C C C G C G G
NarI  (2159)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (2160)
1 site		 G G C G C C C C G C G G
PluTI  (2162)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BGI-F sequencing primer
20-mer  /  45% GC
1 binding site
1173 .. 1192  =  20 annealed bases
Tm  =  53°C
XL39-R sequencing primer
20-mer  /  55% GC
1 binding site
1564 .. 1583  =  20 annealed bases
Tm  =  59°C
AmpR
3074 .. 3934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3074 .. 3142  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3074 .. 3934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3143 .. 3934  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3074 .. 3934  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4105 .. 4693  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4105 .. 4693  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGH poly(A) signal
1501 .. 1977  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
1501 .. 1977  =  477 bp
human growth hormone polyadenylation signal
f1 ori
2232 .. 2687  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2232 .. 2687  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
chimeric intron
873 .. 1251  =  379 bp
fusion between human cytomegalovirus intron A and human β-globin intron 2
chimeric intron
873 .. 1251  =  379 bp
fusion between human cytomegalovirus intron A and human β-globin intron 2
CMV enhancer
217 .. 520  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
217 .. 520  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
521 .. 724  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
521 .. 724  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
1 .. 141  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2017 .. 2157  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AAV2 ITR
2017 .. 2157  =  141 bp
inverted terminal repeat of adeno-associated virus serotype 2
AmpR promoter
2969 .. 3073  =  105 bp
AmpR promoter
2969 .. 3073  =  105 bp
MCS
1324 .. 1408  =  85 bp
multiple cloning site
MCS
1324 .. 1408  =  85 bp
multiple cloning site
Myc
1405 .. 1434  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
1405 .. 1434  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
FLAG
1453 .. 1476  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
1453 .. 1476  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
ORF:  3074 .. 3934  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1815 .. 2255  =  441 bp
ORF:  146 amino acids  =  16.0 kDa
ORF:  1865 .. 2212  =  348 bp
ORF:  115 amino acids  =  13.4 kDa
ORF:  3538 .. 3804  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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