pLMPd-Ametrine

Retroviral vector with an mAmetrine marker, for expressing a short hairpin RNA (shRNA) with an "UltramiR" microRNA scaffold.

Sequence Author: transOMIC

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pLMPd-Ametrine 6740 bp
AmpR
4946 .. 5806  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4946 .. 5737  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4946 .. 5806  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5738 .. 5806  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4946 .. 5806  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
mAmetrine1.1
2294 .. 3013  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 1:  
   2294 .. 2296  =  3 bp
   1 amino acid  =  149.2 Da
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
mAmetrine1.1
2294 .. 3013  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 2:  1a  
   2297 .. 2299  =  3 bp
   1 amino acid  =  117.1 Da
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
mAmetrine1.1
2294 .. 3013  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 3:  
   2300 .. 3013  =  714 bp
   237 amino acids  =  26.6 kDa
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
mAmetrine1.1
2294 .. 3013  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
ori
4187 .. 4775  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4187 .. 4775  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
5' LTR
6739 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
5' LTR
6739 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
3' LTR
3102 .. 3616  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
3' LTR
3102 .. 3616  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
PGK promoter
1774 .. 2273  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
1774 .. 2273  =  500 bp
mouse phosphoglycerate kinase 1 promoter
gag (truncated)
987 .. 1403  =  417 bp
truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon
gag (truncated)
987 .. 1403  =  417 bp
truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
3' miR-30a
1634 .. 1760  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
3' miR-30a
1634 .. 1760  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
1443 .. 1564  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
1443 .. 1564  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
AmpR promoter
5807 .. 5911  =  105 bp
AmpR promoter
5807 .. 5911  =  105 bp
shRNA
1565 .. 1633  =  69 bp
3 segments
   Segment 1:  
   1565 .. 1591  =  27 bp
short hairpin RNA
shRNA
1565 .. 1633  =  69 bp
3 segments
   Segment 2:  miR-30a loop  
   1592 .. 1606  =  15 bp
short hairpin RNA
shRNA
1565 .. 1633  =  69 bp
3 segments
   Segment 3:  
   1607 .. 1633  =  27 bp
short hairpin RNA
shRNA
1565 .. 1633  =  69 bp
3 segments
short hairpin RNA
ORF:  2054 .. 3013  =  960 bp
ORF:  319 amino acids  =  35.4 kDa
ORF:  5076 .. 5342  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  6192 .. 6488  =  297 bp
ORF:  98 amino acids  =  11.7 kDa
ORF:  362 .. 637  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  674 .. 934  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  4946 .. 5806  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2164 .. 2436  =  273 bp
ORF:  90 amino acids  =  9.9 kDa
ORF:  2611 .. 2859  =  249 bp
ORF:  82 amino acids  =  9.1 kDa
ORF:  6256 .. 6486  =  231 bp
ORF:  76 amino acids  =  7.5 kDa
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Download pLMPd-Ametrine.dna file

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