pRFP-C-RS

HuSH-29 retroviral vector with chloramphenicol and puromycin resistance markers and a TurboRFP gene, for expressing an shRNA from the U6 promoter.

Sequence Author: OriGene

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HomePlasmidsViral Expression & Packaging VectorspRFP-C-RS
EcoRI (1) PaqCI (6100) SgrDI (5253) HpaI (5229) CMV enhancer SnaBI (4698) BglII (4390) BclI * (4368) PshAI (3755) AsiSI - PvuI (3673) PmlI (40) BamHI (334) HindIII (380) AvrII (644) BsiWI (729) PciI (2255) DrdI (2363) AgeI (3017) BstXI (3136) BlpI (3236) AleI (3509) FseI (3603) PmeI (3609) PaeR7I - PspXI - XhoI (3614) MluI (3632) BstBI (3660) pRFP-C-RS 7454 bp
EcoRI  (1)
1 site		 G A A T T C C T T A A G
PaqCI  (6100)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SgrDI  (5253)
1 site		 C G T C G A C G G C A G C T G C
HpaI  (5229)
1 site		 G T T A A C C A A T T G
SnaBI  (4698)
1 site		 T A C G T A A T G C A T
BglII  (4390)
1 site		 A G A T C T T C T A G A
BclI  (4368)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PshAI  (3755)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AsiSI  (3673)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (3673)
1 site		 C G A T C G G C T A G C
PmlI  (40)
1 site		 C A C G T G G T G C A C
BamHI  (334)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
HindIII  (380)
1 site		 A A G C T T T T C G A A
AvrII  (644)
1 site		 C C T A G G G G A T C C
BsiWI  (729)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
PciI  (2255)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
DrdI  (2363)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
AgeI  (3017)
1 site		 A C C G G T T G G C C A
BstXI  (3136)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BlpI  (3236)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
AleI  (3509)
1 site		 C A C N N N N G T G G T G N N N N C A C
FseI  (3603)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
PmeI  (3609)
1 site		 G T T T A A A C C A A A T T T G
PaeR7I  (3614)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3614)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (3614)
1 site		 C T C G A G G A G C T C
MluI  (3632)
1 site		 A C G C G T T G C G C A
BstBI  (3660)
1 site		 T T C G A A A A G C T T
TurboRFP
3688 .. 4383  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
TurboRFP
3688 .. 4383  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
CmR
5266 .. 5925  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
5266 .. 5925  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
PuroR
673 .. 1272  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
673 .. 1272  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
3' LTR
1351 .. 1944  =  594 bp
long terminal repeat from Moloney murine leukemia virus
3' LTR
1351 .. 1944  =  594 bp
long terminal repeat from Moloney murine leukemia virus
ori
2316 .. 2904  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2316 .. 2904  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGH poly(A) signal
3091 .. 3567  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
3091 .. 3567  =  477 bp
human growth hormone polyadenylation signal
5' LTR
6100 .. 6569  =  470 bp
long terminal repeat from Moloney murine leukemia virus
5' LTR
6100 .. 6569  =  470 bp
long terminal repeat from Moloney murine leukemia virus
gag (truncated)
7026 .. 7442  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
7026 .. 7442  =  417 bp
truncated MMLV gag gene lacking the start codon
CMV enhancer
4675 .. 5054  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
4675 .. 5054  =  380 bp
human cytomegalovirus immediate early enhancer
U6 promoter
90 .. 330  =  241 bp
Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature.
U6 promoter
90 .. 330  =  241 bp
Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature.
CMV promoter
4471 .. 4674  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
4471 .. 4674  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
MMLV Ψ
6634 .. 6833  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
MMLV Ψ
6634 .. 6833  =  200 bp
packaging signal of Moloney murine leukemia virus (MMLV)
SV40 promoter
463 .. 659  =  197 bp
SV40 early promoter
SV40 promoter
463 .. 659  =  197 bp
SV40 early promoter
shRNA site
334 .. 385  =  52 bp
shRNA insertion site
shRNA site
334 .. 385  =  52 bp
shRNA insertion site
T7 promoter
4427 .. 4445  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
4427 .. 4445  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
510 .. 645  =  136 bp
SV40 origin of replication
SV40 ori
510 .. 645  =  136 bp
SV40 origin of replication
ORF:  673 .. 1272  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  3467 .. 3757  =  291 bp
ORF:  96 amino acids  =  10.4 kDa
ORF:  4037 .. 4282  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  3688 .. 4515  =  828 bp
ORF:  275 amino acids  =  31.4 kDa
ORF:  5266 .. 5925  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

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TA and GC Cloning Vectors
Coronavirus Resources
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pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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