psPAX2

Second generation lentiviral packaging vector from the Trono lab.

Sequence Author: Addgene

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HomePlasmidsViral Expression & Packaging VectorspsPAX2
BsiWI (10,513) BlpI (10,481) EagI (10,337) BsaAI - SnaBI (9724) CMV enhancer PvuI (8816) FspI (8668) PspFI (7861) BseYI (7857) DrdI (7661) PsiI (7260) StuI (7219) SfiI (7173) BsaBI * (7039) PflMI (6590) BmtI (6381) NheI (6377) AleI (5593) SacI (5012) MreI - SgrAI (10,639) BspDI * - ClaI * (480) SphI (1096) BclI * (2078) SbfI (2492) BstZ17I (2578) EcoRV (2628) PflFI - Tth111I (2730) SwaI (3365) AflII (4392) XcmI (4784) Bsu36I (4964) Eco53kI (5010) psPAX2 10,668 bp
BsiWI  (10,513)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
BlpI  (10,481)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
EagI  (10,337)
1 site		 C G G C C G G C C G G C
BsaAI  (9724)
1 site		 Y A C G T R R T G C A Y
SnaBI  (9724)
1 site		 T A C G T A A T G C A T
PvuI  (8816)
1 site		 C G A T C G G C T A G C
FspI  (8668)
1 site		 T G C G C A A C G C G T
PspFI  (7861)
1 site		 C C C A G C G G G T C G
BseYI  (7857)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
DrdI  (7661)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PsiI  (7260)
1 site		 T T A T A A A A T A T T
StuI  (7219)
1 site		 A G G C C T T C C G G A
SfiI  (7173)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BsaBI  (7039)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PflMI  (6590)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BmtI  (6381)
1 site		 G C T A G C C G A T C G
NheI  (6377)
1 site		 G C T A G C C G A T C G
AleI  (5593)
1 site		 C A C N N N N G T G G T G N N N N C A C
SacI  (5012)
1 site		 G A G C T C C T C G A G
MreI  (10,639)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (10,639)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BspDI  (480)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (480)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
SphI  (1096)
1 site		 G C A T G C C G T A C G
BclI  (2078)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SbfI  (2492)
1 site		 C C T G C A G G G G A C G T C C
BstZ17I  (2578)
1 site		 G T A T A C C A T A T G
EcoRV  (2628)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PflFI  (2730)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2730)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
SwaI  (3365)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
AflII  (4392)
1 site		 C T T A A G G A A T T C
XcmI  (4784)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
Bsu36I  (4964)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
Eco53kI  (5010)
1 site		 G A G C T C C T C G A G
HIV-1 pol
1734 .. 4745  =  3012 bp
1003 amino acids  =  113.7 kDa
Product: pol protein from human immunodeficiency virus 1
HIV-1 pol
1734 .. 4745  =  3012 bp
1003 amino acids  =  113.7 kDa
Product: pol protein from human immunodeficiency virus 1
AmpR
8373 .. 9233  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8373 .. 9164  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8373 .. 9233  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   9165 .. 9233  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8373 .. 9233  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
7614 .. 8202  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7614 .. 8202  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
9369 .. 9748  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
9369 .. 9748  =  380 bp
human cytomegalovirus immediate early enhancer
RRE
5319 .. 5552  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
5319 .. 5552  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
SV40 promoter
7040 .. 7235  =  196 bp
SV40 early promoter
SV40 promoter
7040 .. 7235  =  196 bp
SV40 early promoter
SV40 poly(A) signal
7241 .. 7375  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7241 .. 7375  =  135 bp
SV40 polyadenylation signal
AmpR promoter
9234 .. 9338  =  105 bp
AmpR promoter
9234 .. 9338  =  105 bp
β-globin poly(A) signal
6451 .. 6506  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
6451 .. 6506  =  56 bp
rabbit β-globin polyadenylation signal
HIV-1 gag
439 .. 1941  =  1503 bp
500 amino acids  =  55.9 kDa
Product: gag protein from human immunodeficiency virus 1
HIV-1 gag
439 .. 1941  =  1503 bp
500 amino acids  =  55.9 kDa
Product: gag protein from human immunodeficiency virus 1
SV40 ori
7086 .. 7221  =  136 bp
SV40 origin of replication
SV40 ori
7086 .. 7221  =  136 bp
SV40 origin of replication
cPPT/CTS
4430 .. 4547  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
4430 .. 4547  =  118 bp
central polypurine tract and central termination sequence of HIV-1
ORF:  145 .. 399  =  255 bp
ORF:  84 amino acids  =  9.2 kDa
ORF:  439 .. 1941  =  1503 bp
ORF:  500 amino acids  =  55.9 kDa
ORF:  5197 .. 6345  =  1149 bp
ORF:  382 amino acids  =  44.1 kDa
ORF:  8503 .. 8769  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  9658 .. 10,056  =  399 bp
ORF:  132 amino acids  =  14.7 kDa
ORF:  2007 .. 4745  =  2739 bp
ORF:  912 amino acids  =  103.5 kDa
ORF:  10,066 .. 6  =  609 bp
ORF:  202 amino acids  =  21.2 kDa
ORF:  13 .. 312  =  300 bp
ORF:  99 amino acids  =  10.5 kDa
ORF:  5100 .. 5492  =  393 bp
ORF:  130 amino acids  =  14.2 kDa
ORF:  8373 .. 9233  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3224 .. 3499  =  276 bp
ORF:  91 amino acids  =  10.0 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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