pTRIPZ

Empty lentiviral vector for doxycycline-inducible expression of an shRNAmir together with TurboRFP.

Sequence Author: Open Biosystems

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HomePlasmidsViral Expression & Packaging VectorspTRIPZ
SgrDI (13,000) SspI (12,883) FspI (12,301) AhdI (12,079) AanI (10,694) AsiSI (9884) AanI (9054) PmeI (8404) AanI (8354) BsiWI (6732) AanI (13,038) HIV-1 ψ BbvCI (1424) AleI (1577) SgrAI (2500) MauBI (2578) XbaI (2707) tet operator tet operator tet operator tet operator tet operator tet operator minimal CMV promoter AgeI (2992) BsrGI (3042) PasI (3223) TurboRFP BspDI - ClaI (3701) HpaI (3790) PaeR7I - PspXI - XhoI (3805) BamHI (3817) BsaBI * (3822) EcoRI (3829) MluI (4064) AfeI (4125) AanI (4935) SpeI (5231) NheI (5282) BmtI (5286) BamHI (5288) XcmI (5339) BamHI (6076) NotI (6083) pTRIPZ™ 13,320 bp
SgrDI  (13,000)
1 site		 C G T C G A C G G C A G C T G C
SspI  (12,883)
1 site		 A A T A T T T T A T A A
FspI  (12,301)
1 site		 T G C G C A A C G C G T
AhdI  (12,079)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AanI  (10,694)
5 sites		 T T A T A A A A T A T T
AsiSI  (9884)
1 site		 G C G A T C G C C G C T A G C G
AanI  (9054)
5 sites		 T T A T A A A A T A T T
PmeI  (8404)
1 site		 G T T T A A A C C A A A T T T G
AanI  (8354)
5 sites		 T T A T A A A A T A T T
BsiWI  (6732)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
AanI  (13,038)
5 sites		 T T A T A A A A T A T T
BbvCI  (1424)
1 site		 C C T C A G C G G A G T C G
AleI  (1577)
1 site		 C A C N N N N G T G G T G N N N N C A C
SgrAI  (2500)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
MauBI  (2578)
1 site		 C G C G C G C G G C G C G C G C
XbaI  (2707)
1 site		 T C T A G A A G A T C T
AgeI  (2992)
1 site		 A C C G G T T G G C C A
BsrGI  (3042)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
PasI  (3223)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
BspDI  (3701)
1 site		 A T C G A T T A G C T A
ClaI  (3701)
1 site		 A T C G A T T A G C T A
HpaI  (3790)
1 site		 G T T A A C C A A T T G
PaeR7I  (3805)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3805)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (3805)
1 site		 C T C G A G G A G C T C
BamHI  (3817)
3 sites		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsaBI  (3822)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
EcoRI  (3829)
1 site		 G A A T T C C T T A A G
MluI  (4064)
1 site		 A C G C G T T G C G C A
AfeI  (4125)
1 site		 A G C G C T T C G C G A
AanI  (4935)
5 sites		 T T A T A A A A T A T T
SpeI  (5231)
1 site		 A C T A G T T G A T C A
NheI  (5282)
1 site		 G C T A G C C G A T C G
BmtI  (5286)
1 site		 G C T A G C C G A T C G
BamHI  (5288)
3 sites		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XcmI  (5339)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BamHI  (6076)
3 sites		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NotI  (6083)
1 site		 G C G G C C G C C G C C G G C G
UbC promoter
4070 .. 5281  =  1212 bp
human ubiquitin C promoter
UbC promoter
4070 .. 5281  =  1212 bp
human ubiquitin C promoter
HygR (inactivated)
9516 .. 10,545  =  1030 bp
The hygromycin resistance gene was inactivated by destroying an EcoRI site within the coding sequence.
HygR (inactivated)
9516 .. 10,545  =  1030 bp
The hygromycin resistance gene was inactivated by destroying an EcoRI site within the coding sequence.
AmpR
12,006 .. 12,866  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   12,006 .. 12,797  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
12,006 .. 12,866  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   12,798 .. 12,866  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
12,006 .. 12,866  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
rtTA3
5334 .. 6041  =  708 bp
235 amino acids  =  26.3 kDa
Product: reverse tetracycline transactivator 3
activates expression from Tet-responsive promoters in the presence of doxycycline
rtTA3
5334 .. 6041  =  708 bp
235 amino acids  =  26.3 kDa
Product: reverse tetracycline transactivator 3
activates expression from Tet-responsive promoters in the presence of doxycycline
TurboRFP
3005 .. 3700  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
TurboRFP
3005 .. 3700  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
PuroR
6673 .. 7275  =  603 bp
200 amino acids  =  21.6 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
6673 .. 7275  =  603 bp
200 amino acids  =  21.6 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
7291 .. 7879  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
7291 .. 7879  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
11,247 .. 11,835  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
11,247 .. 11,835  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
6098 .. 6666  =  569 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
6098 .. 6666  =  569 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
f1 ori
8696 .. 9124  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
8696 .. 9124  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
BleoR
2245 .. 2619  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
2245 .. 2619  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
SV40 promoter
9138 .. 9467  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
9138 .. 9467  =  330 bp
SV40 enhancer and early promoter
shRNAmir insertion site
3806 .. 4064  =  259 bp
A fragment encoding a microRNA-adapted shRNA can be inserted between the XhoI and MluI sites.
shRNAmir insertion site
3806 .. 4064  =  259 bp
A fragment encoding a microRNA-adapted shRNA can be inserted between the XhoI and MluI sites.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
3' LTR (ΔU3)
8086 .. 8319  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
3' LTR (ΔU3)
8086 .. 8319  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from HIV-1
bGH poly(A) signal
8426 .. 8650  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
8426 .. 8650  =  225 bp
bovine growth hormone polyadenylation signal
SV40 poly(A) signal
13,019 .. 13,153  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
13,019 .. 13,153  =  135 bp
SV40 polyadenylation signal
HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
SV40 poly(A) signal
10,675 .. 10,796  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
10,675 .. 10,796  =  122 bp
SV40 polyadenylation signal
cPPT/CTS
2063 .. 2180  =  118 bp
central polypurine tract and central termination sequence of HIV-1
cPPT/CTS
2063 .. 2180  =  118 bp
central polypurine tract and central termination sequence of HIV-1
AmpR promoter
12,867 .. 12,971  =  105 bp
AmpR promoter
12,867 .. 12,971  =  105 bp
EM7 promoter
2639 .. 2686  =  48 bp
synthetic bacterial promoter
EM7 promoter
2639 .. 2686  =  48 bp
synthetic bacterial promoter
minimal CMV promoter
2930 .. 2968  =  39 bp
human cytomegalovirus (CMV) immediate early promoter
minimal CMV promoter
2930 .. 2968  =  39 bp
human cytomegalovirus (CMV) immediate early promoter
loxP511
5294 .. 5327  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GTATACAT). loxP511 sites are compatible with each other, but incompatible with wild-type loxP sites.
loxP511
5294 .. 5327  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GTATACAT). loxP511 sites are compatible with each other, but incompatible with wild-type loxP sites.
loxP511
6042 .. 6075  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GTATACAT). loxP511 sites are compatible with each other, but incompatible with wild-type loxP sites.
loxP511
6042 .. 6075  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GTATACAT). loxP511 sites are compatible with each other, but incompatible with wild-type loxP sites.
tet operator
2722 .. 2740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2722 .. 2740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2757 .. 2775  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2757 .. 2775  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2793 .. 2811  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2793 .. 2811  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2829 .. 2847  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2829 .. 2847  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2864 .. 2882  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2864 .. 2882  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2900 .. 2918  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2900 .. 2918  =  19 bp
bacterial operator O2 for the tetR and tetA genes
SV40 ori
9318 .. 9453  =  136 bp
SV40 origin of replication
SV40 ori
9318 .. 9453  =  136 bp
SV40 origin of replication
ORF:  5491 .. 5793  =  303 bp
ORF:  100 amino acids  =  11.1 kDa
ORF:  6673 .. 7275  =  603 bp
ORF:  200 amino acids  =  21.6 kDa
ORF:  7381 .. 7908  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  8647 .. 8913  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  9874 .. 10,545  =  672 bp
ORF:  223 amino acids  =  24.9 kDa
ORF:  12,136 .. 12,402  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1181 .. 2068  =  888 bp
ORF:  295 amino acids  =  33.8 kDa
ORF:  2339 .. 2635  =  297 bp
ORF:  98 amino acids  =  10.4 kDa
ORF:  2744 .. 3700  =  957 bp
ORF:  318 amino acids  =  36.1 kDa
ORF:  6581 .. 6970  =  390 bp
ORF:  129 amino acids  =  13.8 kDa
ORF:  7394 .. 7690  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  3717 .. 3962  =  246 bp
ORF:  81 amino acids  =  9.7 kDa
ORF:  5334 .. 6041  =  708 bp
ORF:  235 amino acids  =  26.3 kDa
ORF:  9516 .. 9833  =  318 bp
ORF:  105 amino acids  =  11.7 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  2245 .. 2619  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
ORF:  3106 .. 3351  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  4402 .. 4695  =  294 bp
ORF:  97 amino acids  =  11.3 kDa
ORF:  9625 .. 10,278  =  654 bp
ORF:  217 amino acids  =  24.0 kDa
ORF:  12,006 .. 12,866  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5786 .. 6028  =  243 bp
ORF:  80 amino acids  =  8.2 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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