pLVX-Tight-Puro

Lentiviral vector for expressing a gene with the Tet-On® Advanced or Tet-Off® Advanced system.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspLVX-Tight-Puro
SgrDI (7423) SspI (7306) PvuI (6872) FspI (6724) AhdI (6502) PciI (5609) BmtI (5226) NheI (5222) KpnI (4440) Acc65I (4436) Bsu36I (4432) NcoI (4120) PflMI (3953) FseI (1150) MfeI (1189) BbvCI (1424) AleI (1577) KflI (1934) BspDI - ClaI (2180) PaeR7I - PspXI - XhoI (2203) BamHI (2528) NotI (2535) XbaI * (2548) MluI (2560) EcoRI (2566) SphI (2615) AgeI (2686) SpeI (2821) BlpI (2849) BfuAI - BspMI (3085) PflFI - Tth111I (3138) PasI (3145) BsiWI (3152) RsrII (3212) BstEII (3230) MscI * (3471) CsiI - SexAI * (3663) pLVX-Tight-Puro 7790 bp
SgrDI  (7423)
1 site		 C G T C G A C G G C A G C T G C
SspI  (7306)
1 site		 A A T A T T T T A T A A
PvuI  (6872)
1 site		 C G A T C G G C T A G C
FspI  (6724)
1 site		 T G C G C A A C G C G T
AhdI  (6502)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5609)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BmtI  (5226)
1 site		 G C T A G C C G A T C G
NheI  (5222)
1 site		 G C T A G C C G A T C G
KpnI  (4440)
1 site		 G G T A C C C C A T G G
Acc65I  (4436)
1 site		 G G T A C C C C A T G G
Bsu36I  (4432)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NcoI  (4120)
1 site		 C C A T G G G G T A C C
PflMI  (3953)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
FseI  (1150)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
MfeI  (1189)
1 site		 C A A T T G G T T A A C
BbvCI  (1424)
1 site		 C C T C A G C G G A G T C G
AleI  (1577)
1 site		 C A C N N N N G T G G T G N N N N C A C
KflI  (1934)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site		 A T C G A T T A G C T A
ClaI  (2180)
1 site		 A T C G A T T A G C T A
PaeR7I  (2203)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2203)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (2203)
1 site		 C T C G A G G A G C T C
BamHI  (2528)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NotI  (2535)
1 site		 G C G G C C G C C G C C G G C G
XbaI  (2548)
1 site		 T C T A G A A G A T C T
* Blocked by Dam methylation.
MluI  (2560)
1 site		 A C G C G T T G C G C A
EcoRI  (2566)
1 site		 G A A T T C C T T A A G
SphI  (2615)
1 site		 G C A T G C C G T A C G
AgeI  (2686)
1 site		 A C C G G T T G G C C A
SpeI  (2821)
1 site		 A C T A G T T G A T C A
BlpI  (2849)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BfuAI  (3085)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3085)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PflFI  (3138)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3138)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PasI  (3145)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
BsiWI  (3152)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3212)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (3230)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
MscI  (3471)
1 site		 T G G C C A A C C G G T
* Blocked by Dcm methylation.
CsiI  (3663)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (3663)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AmpR
6429 .. 7289  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6429 .. 7220  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6429 .. 7289  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7221 .. 7289  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6429 .. 7289  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
3' LTR
4507 .. 5140  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
4507 .. 5140  =  634 bp
3' long terminal repeat (LTR) from HIV-1
PuroR
3096 .. 3695  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
3096 .. 3695  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
3712 .. 4300  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
WPRE
3712 .. 4300  =  589 bp
woodchuck hepatitis virus posttranscriptional regulatory element
ori
5670 .. 6258  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5670 .. 6258  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
2576 .. 3075  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
2576 .. 3075  =  500 bp
mouse phosphoglycerate kinase 1 promoter
tight TRE promoter
2206 .. 2520  =  315 bp
Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter
tight TRE promoter
2206 .. 2520  =  315 bp
Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
SV40 poly(A) signal
7442 .. 7576  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
7442 .. 7576  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
7290 .. 7394  =  105 bp
AmpR promoter
7290 .. 7394  =  105 bp
MCS
2528 .. 2571  =  44 bp
multiple cloning site
MCS
2528 .. 2571  =  44 bp
multiple cloning site
tet operator
2214 .. 2232  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2214 .. 2232  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2250 .. 2268  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2250 .. 2268  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2285 .. 2303  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2285 .. 2303  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2321 .. 2339  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2321 .. 2339  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2357 .. 2375  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2357 .. 2375  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2392 .. 2410  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2392 .. 2410  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2428 .. 2446  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
2428 .. 2446  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  2272 .. 2799  =  528 bp
ORF:  175 amino acids  =  19.8 kDa
ORF:  3802 .. 4329  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  6559 .. 6825  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1181 .. 2032  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  3815 .. 4111  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  2856 .. 3695  =  840 bp
ORF:  279 amino acids  =  30.1 kDa
ORF:  6429 .. 7289  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
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Download pLVX-Tight-Puro.dna file

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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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