pRS
HuSH-29 retroviral vector with ampicillin and puromycin resistance markers, for expressing an shRNA from the U6 promoter.
Sequence Author: OriGene
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Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
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Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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Sticky ends from different DraIII sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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* Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different AflIII sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different DrdI sites may not be compatible. |
AmpR 3082 .. 3942 = 861 bp 286 amino acids = 31.5 kDa 2 segments Segment 2: 3082 .. 3873 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3082 .. 3942 = 861 bp 286 amino acids = 31.5 kDa 2 segments Segment 1: signal sequence 3874 .. 3942 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3082 .. 3942 = 861 bp 286 amino acids = 31.5 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
3' LTR 1351 .. 1944 = 594 bp long terminal repeat from Moloney murine leukemia virus |
3' LTR 1351 .. 1944 = 594 bp long terminal repeat from Moloney murine leukemia virus |
ori 2316 .. 2904 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 2316 .. 2904 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
5' LTR 4078 .. 4547 = 470 bp long terminal repeat from Moloney murine leukemia virus |
5' LTR 4078 .. 4547 = 470 bp long terminal repeat from Moloney murine leukemia virus |
gag (truncated) 5004 .. 5420 = 417 bp truncated MMLV gag gene lacking the start codon |
gag (truncated) 5004 .. 5420 = 417 bp truncated MMLV gag gene lacking the start codon |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
MMLV Ψ 4612 .. 4811 = 200 bp packaging signal of Moloney murine leukemia virus (MMLV) |
MMLV Ψ 4612 .. 4811 = 200 bp packaging signal of Moloney murine leukemia virus (MMLV) |
SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
AmpR promoter 3943 .. 4047 = 105 bp |
AmpR promoter 3943 .. 4047 = 105 bp |
shRNA site 334 .. 385 = 52 bp shRNA insertion site |
shRNA site 334 .. 385 = 52 bp shRNA insertion site |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
ORF: 673 .. 1272 = 600 bp ORF: 199 amino acids = 21.5 kDa |
ORF: 3212 .. 3478 = 267 bp ORF: 88 amino acids = 9.2 kDa |
ORF: 3082 .. 3942 = 861 bp ORF: 286 amino acids = 31.5 kDa |
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Sticky ends from different DraIII sites may not be compatible. |
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Click here to try SnapGene |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
Click here to try SnapGene |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
Click here to try SnapGene |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
Click here to try SnapGene |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
U6 promoter 90 .. 330 = 241 bp Truncated RNA polymerase III promoter for human U6 snRNA used in pSIREN vectors such as pSIREN-RetroQ-ZsGreen1. Expected transcription start site is 8bp downstream of this feature. |
shRNA site 334 .. 385 = 52 bp shRNA insertion site |
shRNA site 334 .. 385 = 52 bp shRNA insertion site |
Click here to try SnapGene |
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SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
Click here to try SnapGene |
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SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
Click here to try SnapGene |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
Click here to try SnapGene |
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SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
SV40 promoter 463 .. 659 = 197 bp SV40 early promoter |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
SV40 ori 510 .. 645 = 136 bp SV40 origin of replication |
Click here to try SnapGene |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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* Blocked by Dam methylation. |
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
Click here to try SnapGene |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
Click here to try SnapGene |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
Click here to try SnapGene |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
Click here to try SnapGene |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
Click here to try SnapGene |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 673 .. 1272 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
Click here to try SnapGene |
Download pRS.dna file
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