pAdenoX-Tet3G Linear

In-Fusion®-ready linearized adenoviral vector for doxycycline-inducible expression of a gene.

Sequence Author: Clontech (TaKaRa)

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors | More Plasmid Sets
No matches
HomePlasmidsViral Expression & Packaging VectorspAdenoX-Tet3G Linear
End (36,043) In-Fusion Cloning site Ad5 Ψ Adeno-X fwd screening primer (35,368 .. 35,392) M13 rev AmpR promoter M13 fwd BstBI (32,297) SV40 poly(A) signal CMV promoter I-PpoI (25,302) SrfI (24,953) MreI (23,818) AsiSI (18,871) Start (0) In-Fusion Cloning site stop codons small t intron Adeno-X rev screening primer (936 .. 955) BstZ17I (3197) PmeI (10,687) pAdenoX-Tet3G Linear 36,043 bp
End  (36,043)
0 sites
BstBI  (32,297)
1 site		 T T C G A A A A G C T T
I-PpoI  (25,302)
1 site		 C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G
I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences.
SrfI  (24,953)
1 site		 G C C C G G G C C G G G C C C G
MreI  (23,818)
1 site		 C G C C G G C G G C G G C C G C
AsiSI  (18,871)
1 site		 G C G A T C G C C G C T A G C G
Start  (0)
0 sites
BstZ17I  (3197)
1 site		 G T A T A C C A T A T G
PmeI  (10,687)
1 site		 G T T T A A A C C A A A T T T G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
35,368 .. 35,392  =  25 annealed bases
Tm  =  64°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
936 .. 955  =  20 annealed bases
Tm  =  56°C
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
956 .. 25,286  =  24,331 bp
adenovirus DNA
27,337 .. 32,172  =  4836 bp
adenovirus DNA
27,337 .. 32,172  =  4836 bp
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   33,176 .. 33,244  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   33,245 .. 34,036  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
33,176 .. 34,036  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
Tet-On 3G
26,097 .. 26,843  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
Tet-On 3G
26,097 .. 26,843  =  747 bp
248 amino acids  =  27.7 kDa
Product: modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline
ori
34,207 .. 34,795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
34,207 .. 34,795  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
32,537 .. 32,992  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
32,537 .. 32,992  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
25,411 .. 25,790  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
25,411 .. 25,790  =  380 bp
human cytomegalovirus immediate early enhancer
TRE3G promoter
35,645 .. 36,020  =  376 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G
TRE3G promoter
35,645 .. 36,020  =  376 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On 3G
CMV promoter
25,791 .. 25,994  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
25,791 .. 25,994  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
Ad5 Ψ
35,455 .. 35,605  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
35,455 .. 35,605  =  151 bp
packaging signal for adenovirus serotype 5
SV40 poly(A) signal
26,853 .. 26,987  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
26,853 .. 26,987  =  135 bp
SV40 polyadenylation signal
AmpR promoter
33,071 .. 33,175  =  105 bp
AmpR promoter
33,071 .. 33,175  =  105 bp
ITR
32,173 .. 32,275  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
32,173 .. 32,275  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
35,265 .. 35,367  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
35,265 .. 35,367  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
822 .. 903  =  82 bp
SV40 polyadenylation signal
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
182 .. 247  =  66 bp
simian virus 40 (SV40) small t antigen intron
M13 fwd
32,380 .. 32,396  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
32,380 .. 32,396  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
35,181 .. 35,197  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
35,181 .. 35,197  =  17 bp
common sequencing primer, one of multiple similar variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
36,029 .. 36,043  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
36,029 .. 36,043  =  15 bp
sequence to be added to 5' end of forward primer
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
stop codons
35 .. 45  =  11 bp
stop codons in all three reading frames
tet operator
35,650 .. 35,668  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,650 .. 35,668  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,686 .. 35,704  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,686 .. 35,704  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,722 .. 35,740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,722 .. 35,740  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,758 .. 35,776  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,758 .. 35,776  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,794 .. 35,812  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,794 .. 35,812  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,830 .. 35,848  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,830 .. 35,848  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,866 .. 35,884  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
35,866 .. 35,884  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  805 .. 1059  =  255 bp
ORF:  84 amino acids  =  9.9 kDa
ORF:  3721 .. 4041  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  4741 .. 4992  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  5926 .. 6162  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  8479 .. 9726  =  1248 bp
ORF:  415 amino acids  =  47.0 kDa
ORF:  11,413 .. 11,643  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  14,716 .. 15,195  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  15,433 .. 16,185  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  19,162 .. 19,776  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  20,908 .. 23,913  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  26,254 .. 26,556  =  303 bp
ORF:  100 amino acids  =  11.1 kDa
ORF:  27,367 .. 29,124  =  1758 bp
ORF:  585 amino acids  =  62.1 kDa
ORF:  326 .. 799  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  1040 .. 1462  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  7862 .. 8242  =  381 bp
ORF:  126 amino acids  =  13.5 kDa
ORF:  16,271 .. 19,129  =  2859 bp
ORF:  952 amino acids  =  107.9 kDa
ORF:  21,164 .. 21,520  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  21,863 .. 22,249  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  23,426 .. 24,208  =  783 bp
ORF:  260 amino acids  =  28.8 kDa
ORF:  29,072 .. 29,470  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  30,293 .. 30,790  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  33,176 .. 34,036  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5409 .. 5858  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  6735 .. 7241  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  9747 .. 11,504  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  11,586 .. 13,301  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  13,308 .. 13,904  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  13,941 .. 15,080  =  1140 bp
ORF:  379 amino acids  =  42.7 kDa
ORF:  15,108 .. 15,350  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  24,597 .. 25,280  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  25,950 .. 26,843  =  894 bp
ORF:  297 amino acids  =  33.8 kDa
ORF:  27,078 .. 27,332  =  255 bp
ORF:  84 amino acids  =  9.0 kDa
ORF:  30,804 .. 31,142  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  35,673 .. 35,939  =  267 bp
ORF:  88 amino acids  =  10.5 kDa
ORF:  2768 .. 3115  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  6014 .. 7975  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  8183 .. 8548  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  9743 .. 10,003  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  14,627 .. 14,878  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  15,077 .. 15,367  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  26,549 .. 27,004  =  456 bp
ORF:  151 amino acids  =  16.0 kDa
ORF:  30,335 .. 30,679  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  31,037 .. 31,447  =  411 bp
ORF:  136 amino acids  =  15.3 kDa
ORF:  1522 .. 2880  =  1359 bp
ORF:  452 amino acids  =  51.1 kDa
ORF:  3883 .. 4221  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  5893 .. 6471  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  8689 .. 9057  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  16,303 .. 16,578  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  29,530 .. 30,414  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
ORF:  31,477 .. 31,863  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  33,640 .. 33,906  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1905 .. 2129  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  2628 .. 5798  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  7731 .. 8144  =  414 bp
ORF:  137 amino acids  =  15.3 kDa
ORF:  8988 .. 9281  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  11,292 .. 11,549  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  11,811 .. 12,596  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  14,073 .. 14,342  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  15,591 .. 16,223  =  633 bp
ORF:  210 amino acids  =  22.5 kDa
ORF:  19,872 .. 21,461  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  23,856 .. 24,146  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  26,952 .. 27,368  =  417 bp
ORF:  138 amino acids  =  15.1 kDa
ORF:  27,495 .. 27,965  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  28,299 .. 28,544  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  30,690 .. 31,040  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  32,775 .. 33,020  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
Click here to try SnapGene

Download pAdenoX-Tet3G Linear.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free