pAdenoX-PRLS-ZsGreen1 Linear

In-Fusion®-ready linearized promoterless adenoviral vector for inserting a gene cassette and co-expressing with ZsGreen1.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspAdenoX-PRLS-ZsGreen1 Linear
End (34,322) In-Fusion Cloning site Adeno-X fwd screening primer (34,060 .. 34,084) M13 rev AmpR promoter BstBI (30,989) SV40 poly(A) signal CMV promoter I-PpoI (24,385) SrfI (24,036) SpeI (23,588) MreI (22,901) AsiSI (17,954) Start (0) Adeno-X rev screening primer (19 .. 38) BstZ17I (2280) PmeI (9770) pAdenoX-PRLS-ZsGreen1 Linear 34,322 bp
End  (34,322)
0 sites
BstBI  (30,989)
1 site		 T T C G A A A A G C T T
I-PpoI  (24,385)
1 site		 C T C T C T T A A G G T A G C G A G A G A A T T C C A T C G
I-PpoI is a homing endonuclease that can recognize a variety of similar recognition sequences.
SrfI  (24,036)
1 site		 G C C C G G G C C G G G C C C G
SpeI  (23,588)
1 site		 A C T A G T T G A T C A
MreI  (22,901)
1 site		 C G C C G G C G G C G G C C G C
AsiSI  (17,954)
1 site		 G C G A T C G C C G C T A G C G
Start  (0)
0 sites
BstZ17I  (2280)
1 site		 G T A T A C C A T A T G
PmeI  (9770)
1 site		 G T T T A A A C C A A A T T T G
Adeno-X fwd screening primer
25-mer  /  52% GC
1 binding site
34,060 .. 34,084  =  25 annealed bases
Tm  =  64°C
Adeno-X rev screening primer
20-mer  /  50% GC
1 binding site
19 .. 38  =  20 annealed bases
Tm  =  56°C
adenovirus DNA
39 .. 24,369  =  24,331 bp
adenovirus DNA
39 .. 24,369  =  24,331 bp
adenovirus DNA
26,029 .. 30,864  =  4836 bp
adenovirus DNA
26,029 .. 30,864  =  4836 bp
AmpR
31,868 .. 32,728  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   31,868 .. 31,936  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
31,868 .. 32,728  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   31,937 .. 32,728  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
31,868 .. 32,728  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ZsGreen1
25,049 .. 25,744  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
25,049 .. 25,744  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ori
32,899 .. 33,487  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
32,899 .. 33,487  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
31,229 .. 31,684  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
31,229 .. 31,684  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
24,496 .. 24,799  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
24,496 .. 24,799  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
24,800 .. 25,003  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
24,800 .. 25,003  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
Ad5 Ψ
34,147 .. 34,297  =  151 bp
packaging signal for adenovirus serotype 5
Ad5 Ψ
34,147 .. 34,297  =  151 bp
packaging signal for adenovirus serotype 5
AmpR promoter
31,763 .. 31,867  =  105 bp
AmpR promoter
31,763 .. 31,867  =  105 bp
ITR
30,865 .. 30,967  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
30,865 .. 30,967  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
33,957 .. 34,059  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
ITR
33,957 .. 34,059  =  103 bp
inverted terminal repeat of human adenovirus serotype 5
SV40 poly(A) signal
25,866 .. 25,947  =  82 bp
SV40 polyadenylation signal
SV40 poly(A) signal
25,866 .. 25,947  =  82 bp
SV40 polyadenylation signal
M13 fwd
31,072 .. 31,088  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
31,072 .. 31,088  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
33,873 .. 33,889  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
33,873 .. 33,889  =  17 bp
common sequencing primer, one of multiple similar variants
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
1 .. 15  =  15 bp
sequence to be added to 5' end of reverse primer
In-Fusion Cloning site
34,308 .. 34,322  =  15 bp
sequence to be added to 5' end of forward primer
In-Fusion Cloning site
34,308 .. 34,322  =  15 bp
sequence to be added to 5' end of forward primer
ORF:  4492 .. 4941  =  450 bp
ORF:  149 amino acids  =  16.7 kDa
ORF:  5818 .. 6324  =  507 bp
ORF:  168 amino acids  =  17.7 kDa
ORF:  8830 .. 10,587  =  1758 bp
ORF:  585 amino acids  =  65.3 kDa
ORF:  10,669 .. 12,384  =  1716 bp
ORF:  571 amino acids  =  63.3 kDa
ORF:  12,391 .. 12,987  =  597 bp
ORF:  198 amino acids  =  22.0 kDa
ORF:  13,024 .. 14,163  =  1140 bp
ORF:  379 amino acids  =  42.7 kDa
ORF:  14,191 .. 14,433  =  243 bp
ORF:  80 amino acids  =  8.8 kDa
ORF:  23,680 .. 24,363  =  684 bp
ORF:  227 amino acids  =  24.7 kDa
ORF:  26,059 .. 27,816  =  1758 bp
ORF:  585 amino acids  =  62.1 kDa
ORF:  2804 .. 3124  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  3824 .. 4075  =  252 bp
ORF:  83 amino acids  =  9.4 kDa
ORF:  5009 .. 5245  =  237 bp
ORF:  78 amino acids  =  7.9 kDa
ORF:  7562 .. 8809  =  1248 bp
ORF:  415 amino acids  =  47.0 kDa
ORF:  10,496 .. 10,726  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  13,799 .. 14,278  =  480 bp
ORF:  159 amino acids  =  17.4 kDa
ORF:  14,516 .. 15,268  =  753 bp
ORF:  250 amino acids  =  27.0 kDa
ORF:  18,245 .. 18,859  =  615 bp
ORF:  204 amino acids  =  23.1 kDa
ORF:  19,991 .. 22,996  =  3006 bp
ORF:  1001 amino acids  =  110.0 kDa
ORF:  25,049 .. 25,744  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
ORF:  27,764 .. 28,162  =  399 bp
ORF:  132 amino acids  =  15.0 kDa
ORF:  28,985 .. 29,482  =  498 bp
ORF:  165 amino acids  =  18.7 kDa
ORF:  31,868 .. 32,728  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  123 .. 545  =  423 bp
ORF:  140 amino acids  =  14.5 kDa
ORF:  6945 .. 7325  =  381 bp
ORF:  126 amino acids  =  13.5 kDa
ORF:  15,354 .. 18,212  =  2859 bp
ORF:  952 amino acids  =  107.9 kDa
ORF:  20,247 .. 20,603  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  20,946 .. 21,332  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  22,509 .. 23,291  =  783 bp
ORF:  260 amino acids  =  28.8 kDa
ORF:  29,496 .. 29,834  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  1851 .. 2198  =  348 bp
ORF:  115 amino acids  =  12.2 kDa
ORF:  5097 .. 7058  =  1962 bp
ORF:  653 amino acids  =  74.7 kDa
ORF:  7266 .. 7631  =  366 bp
ORF:  121 amino acids  =  12.7 kDa
ORF:  8826 .. 9086  =  261 bp
ORF:  86 amino acids  =  9.0 kDa
ORF:  13,710 .. 13,961  =  252 bp
ORF:  83 amino acids  =  8.3 kDa
ORF:  14,160 .. 14,450  =  291 bp
ORF:  96 amino acids  =  10.9 kDa
ORF:  26,187 .. 26,657  =  471 bp
ORF:  156 amino acids  =  16.6 kDa
ORF:  26,991 .. 27,236  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  29,382 .. 29,732  =  351 bp
ORF:  116 amino acids  =  13.3 kDa
ORF:  31,467 .. 31,712  =  246 bp
ORF:  81 amino acids  =  8.6 kDa
ORF:  605 .. 1963  =  1359 bp
ORF:  452 amino acids  =  51.1 kDa
ORF:  2966 .. 3304  =  339 bp
ORF:  112 amino acids  =  12.8 kDa
ORF:  4976 .. 5554  =  579 bp
ORF:  192 amino acids  =  20.8 kDa
ORF:  7772 .. 8140  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  15,386 .. 15,661  =  276 bp
ORF:  91 amino acids  =  10.2 kDa
ORF:  29,027 .. 29,371  =  345 bp
ORF:  114 amino acids  =  13.3 kDa
ORF:  29,729 .. 30,139  =  411 bp
ORF:  136 amino acids  =  15.3 kDa
ORF:  988 .. 1212  =  225 bp
ORF:  74 amino acids  =  8.3 kDa
ORF:  1711 .. 4881  =  3171 bp
ORF:  1056 amino acids  =  120.4 kDa
ORF:  6814 .. 7227  =  414 bp
ORF:  137 amino acids  =  15.3 kDa
ORF:  8071 .. 8364  =  294 bp
ORF:  97 amino acids  =  10.5 kDa
ORF:  10,375 .. 10,632  =  258 bp
ORF:  85 amino acids  =  9.5 kDa
ORF:  10,894 .. 11,679  =  786 bp
ORF:  261 amino acids  =  28.5 kDa
ORF:  13,156 .. 13,425  =  270 bp
ORF:  89 amino acids  =  9.5 kDa
ORF:  14,674 .. 15,306  =  633 bp
ORF:  210 amino acids  =  22.5 kDa
ORF:  18,955 .. 20,544  =  1590 bp
ORF:  529 amino acids  =  59.1 kDa
ORF:  22,939 .. 23,229  =  291 bp
ORF:  96 amino acids  =  10.5 kDa
ORF:  25,426 .. 25,719  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  28,222 .. 29,106  =  885 bp
ORF:  294 amino acids  =  34.1 kDa
ORF:  30,169 .. 30,555  =  387 bp
ORF:  128 amino acids  =  14.3 kDa
ORF:  32,332 .. 32,598  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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